Immunohistochemistry Resource Guide
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Table of Contents
Immunohistochemistry Selection Guide
Avidin-Biotin Complex (ABC)-Based Detection
Choosing a VECTASTAIN® ABC Kit
> Customizing your VECTASTAIN® ABC Kit
ImmPRESS® One-Step Polymer Systems
> ImmPRESS® Two-Step Amplified Polymer Systems
> Choosing an ImmPRESS® Polymer Kit
> Multiple Antigen Labeling Simplified
M.O.M.® (Mouse on Mouse) Immunodetection Kits
> Counterstain/Substrate Compatibility Table
Enzyme Substrate Properties
See also Enzyme Substrate Combinations for multiple antigen labeling and Counterstain/Substrate Compatibility.
| Substrate | Color | Catalog Number | Microscopy | Mounting | Contrast in Pigmented Tissue | Multiple Labeling | Heat Resistant* | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Bright- field | Darkfield | Electron | Fluore- scence | Spectral Imaging | |||||||
| Peroxidase | |||||||||||
| Vector® DAB | Brown | SK-4100 | • | • | • | • | Non-aqueous or Aqueous | • | • | ||
| Vector® DAB +Ni | Gray-Black | SK-4100 | • | • | • | • | Non-aqueous | • | |||
| ImmPACT® DAB | Brown | SK-4105 | • | • | • | • | Non-aqueous or Aqueous | • | • | ||
| ImmPACT® DAB EqV | Brown | SK-4103 | • | • | • | • | Non-aqueous or Aqueous | • | • | ||
| Vector® VIP | Purple | SK-4600 | • | • | • | • | Non-aqueous | • | • | ||
| ImmPACT® VIP | Purple | SK-4605 | • | • | • | • | Non-aqueous | • | • | ||
| Vector® SG | Blue-Gray | SK-4700 | • | • | • | • | Non-aqueous or Aqueous | • | • | ||
| ImmPACT® SG | Blue-Gray | SK-4705 | • | • | • | • | Non-aqueous or Aqueous | • | • | ||
| Vector® NovaRED® | Red | SK-4800 | • | • | • | • | Non-aqueous | • | • | ||
| ImmPACT® NovaRED® | Red | SK-4805 | • | • | • | • | Non-aqueous | • | • | ||
| Vector® AEC | Red | SK-4200 | • | • | Aqueous | • | • | ||||
| ImmPACT® AEC | Red | SK-4205 | • | • | Aqueous | • | • | ||||
| ImmPACT® AMEC Red | Red | SK-4285 | • | • | Aqueous | • | • | ||||
| TMB | Blue | SK-4400 | • | • | Non-aqueous | ||||||
| Alkaline Phosphatase | |||||||||||
| Vector® Red | Magenta | SK-5100 | • | • | • | Non-aqueous or Aqueous | • | • | • | ||
| ImmPACT® Vector® Red | Magenta | SK-5105 | • | • | • | Non-aqueous or Aqueous | • | • | • | ||
| Vector® Blue | Blue | SK-5300 | • | • | • | Non-aqueous or Aqueous | • | • | • | ||
| Vector® Black | Brown-Black | SK-5200 | • | Non-aqueous | |||||||
| BCIP/NBT | Indigo | SK-5400 | • | • | • | Non-aqueous or Aqueous | • | • | |||
* Substrates that are designated “heat resistant” were developed on tissue then subjected to heat induced epitope retrival (HIER) using a pressure cooker technique (stained tissue was pressure cooked for 1 minute in Antigen Unmasking Solution, returned to room temperature, and rinsed in buffer). Resulting sensitivity after this treatment was found to be equivalent to non-HIER treated tissue.
Prostate: Prostate Specific Antigen (m), ImmPRESS Reagent (HRP), ImmPACT DAB (brown).d), Hematoxylin QS (blue).
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP), Vector DAB-Ni (gray-black).
Tonsil: Cytokeratin AE1/AE3 (m), Vector ImmPRESS- AP Reagent, ImmPACT Vector Red (magenta).
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP), ImmPACT SG (blue-gray).ylin QS (blue).
Tumor: Cytokeratin (s), VECTASTAIN® Elite® ABC Kit, TMB (blue).
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS-AP Reagent, Vector Blue (blue).
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP), ImmPACT NovaRED (red).EC (red), Hematoxylin QS (blue).
Prostate: Prostate Specific Antigen (m), ImmPRESS Reagent (HRP), ImmPACT AEC (red).
Colon Carcinoma: Pan-Cytokeratin (m), VECTASTAIN ABC-AP Kit, Vector Black (brown-black).
Tonsil: CD20 (m), ImmPRESS Reagent (HRP), ImmPACT VIP (purple).
Tonsil: LCA (m), ImmPRESS Reagent (HRP), ImmPACT AMEC Red (red).
Prostate: Prostate Specific Antigen (m), VECTASTAIN ABC-AP Kit, BCIP/NBT (indigo).
