Table of Contents
Enzyme Substrate Properties
See also Enzyme Substrate Combinations for multiple antigen labeling and Counterstain/Substrate Compatibility.
| Substrate | Color | Catalog Number | Microscopy | Mounting | Contrast in Pigmented Tissue | Multiple Labeling | Heat Resistant* | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Bright- field | Darkfield | Electron | Fluore- scence | Spectral Imaging | |||||||
| Peroxidase | |||||||||||
| Vector® DAB | Brown | SK-4100 |
• |
• |
• |
|
• |
Non-aqueous or Aqueous |
|
• |
• |
| Vector® DAB +Ni | Gray-Black | SK-4100 |
• |
• |
• |
|
• |
Non-aqueous |
|
• |
|
| ImmPACT® DAB | Brown | SK-4105 |
• |
• |
• |
|
• |
Non-aqueous or Aqueous |
|
• |
• |
| ImmPACT® DAB EqV | Brown | SK-4103 |
• |
• |
• |
|
• |
Non-aqueous or Aqueous |
|
• |
• |
| Vector® VIP | Purple | SK-4600 |
• |
• |
• |
|
• |
Non-aqueous |
• |
• |
|
| ImmPACT® VIP | Purple | SK-4605 |
• |
• |
• |
|
• |
Non-aqueous |
• |
• |
|
| Vector® SG | Blue-Gray | SK-4700 |
• |
• |
• |
|
• |
Non-aqueous or Aqueous |
• |
• |
|
| ImmPACT® SG | Blue-Gray | SK-4705 |
• |
• |
• |
|
• |
Non-aqueous or Aqueous |
• |
• |
|
| Vector® NovaRED® | Red | SK-4800 |
• |
• |
• |
|
• |
Non-aqueous |
• |
• |
|
| ImmPACT® NovaRED® | Red | SK-4805 |
• |
• |
• |
|
• |
Non-aqueous |
• |
• |
|
| Vector® AEC | Red | SK-4200 |
• |
|
|
|
• |
Aqueous |
• |
• |
|
| ImmPACT® AEC | Red | SK-4205 |
• |
|
|
|
• |
Aqueous |
• |
• |
|
| ImmPACT® AMEC Red | Red | SK-4285 |
• |
|
|
|
• |
Aqueous |
• |
• |
|
| TMB | Blue | SK-4400 |
• |
|
|
|
• |
Non-aqueous |
|
|
|
| Alkaline Phosphatase | |||||||||||
| Vector® Red | Magenta | SK-5100 |
• |
|
|
• |
• |
Non-aqueous or Aqueous |
• |
• |
• |
|
ImmPACT® Vector® Red |
Magenta | SK-5105 |
• |
|
|
• |
• |
Non-aqueous or Aqueous |
• |
• |
• |
| Vector® Blue | Blue | SK-5300 |
• |
|
|
• |
• |
Non-aqueous or Aqueous |
• |
• |
• |
| Vector® Black | Brown-Black | SK-5200 |
• |
|
|
|
|
Non-aqueous |
|
|
|
| BCIP/NBT | Indigo | SK-5400 |
• |
|
|
• |
• |
Non-aqueous or Aqueous |
|
• |
• |
* Substrates that are designated “heat resistant” were developed on tissue then subjected to heat induced epitope retrival (HIER) using a pressure cooker technique (stained tissue was pressure cooked for 1 minute in Antigen Unmasking Solution, returned to room temperature, and rinsed in buffer). Resulting sensitivity after this treatment was found to be equivalent to non-HIER treated tissue.
Peroxidase
Substrates
.
.
Alkaline Phosphatase Substrates
.
.
