Table of Contents
- Introduction
- Immunohistochemistry Workflow
- Immunohistochemistry Selection Guide
- Pioneering in IHC Technology
- Choosing a Detection System
- Avidin-Biotin Complex (ABC)-Based Detection
- VECTASTAIN® ABC Systems
- VECTASTAIN® ABC Kits
- Choosing a VECTASTAIN® ABC Kit
- > Customizing your VECTASTAIN® ABC Kit
- Polymer-Based Detection
- ImmPRESS® One-Step Polymer Systems
- > ImmPRESS® Two-Step Amplified Polymer Systems
- > Choosing an ImmPRESS® Polymer Kit
- > Multiple Antigen Labeling Simplified
- Species on Species Detection
- M.O.M.® (Mouse on Mouse) Immunodetection Kits
- Choosing an Enzyme Substrate
- Enzyme Substrates
- > Enzyme Substrate Properties
- Multiple Antigen Labeling
- Enzyme Substrate Combinations
- Counterstaining
- > Counterstain/Substrate Compatibility Table
- Blocking Background Signal
- Secondary and Tertiary Detection Reagents
- Mounting Media
- Accessory Reagents
- Introduction
- Immunohistochemistry Workflow
- Immunohistochemistry Selection Guide
- Pioneering in IHC Technology
- Choosing a Detection System
- Avidin-Biotin Complex (ABC)-Based Detection
- VECTASTAIN® ABC Systems
- VECTASTAIN® ABC Kits
- Choosing a VECTASTAIN® ABC Kit
- > Customizing your VECTASTAIN® ABC Kit
- Polymer-Based Detection
- ImmPRESS® One-Step Polymer Systems
- > ImmPRESS® Two-Step Amplified Polymer Systems
- > Choosing an ImmPRESS® Polymer Kit
- > Multiple Antigen Labeling Simplified
- Species on Species Detection
- M.O.M.® (Mouse on Mouse) Immunodetection Kits
- Choosing an Enzyme Substrate
- Enzyme Substrates
- > Enzyme Substrate Properties
- Multiple Antigen Labeling
- Enzyme Substrate Combinations
- Counterstaining
- > Counterstain/Substrate Compatibility Table
- Blocking Background Signal
- Secondary and Tertiary Detection Reagents
- Mounting Media
- Accessory Reagents
Counterstain/Substrate Compatibility Table
This table is designed as a reference to determine the optimal counterstain/substrate combination for your application. Considerations should be given to tissue type, antigen unmasking protocol and other detection parameters to achieve the desired staining intensity.
Substrate | Catalog Number | Vector® Hematoxylin and Hematoxylin QS H-3401 and H-3404 |
Vector® Methyl Green H-3402 | Vector® Nuclear Fast Red H-3403 |
---|---|---|---|---|
ImmPACT® DAB EqV | SK-4103 | Excellent Contrast | Excellent Contrast | Fair Contrast |
ImmPACT® DAB (brown) | SK-4105 | Excellent Contrast | Excellent Contrast | Fair Contrast |
Vector® DAB (brown) | SK-4100 | Excellent Contrast | Excellent Contrast | Fair Contrast |
Vector® DAB-Ni (gray-black) | SK-4100 | Excellent Contrast | Fair Contrast * | Good Contrast |
ImmPACT® AEC (red) | SK-4205 | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility |
ImmPACT® AMEC Red (red) | SK-4285 | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility |
Vector® AEC (red) | SK-4200 | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility |
TMB (blue) | SK-4400 | Color Incompatibility | Counterstain Incompatibility | Excellent Contrast |
ImmPACT® VIP (purple) | SK-4605 | Fair Contrast | Excellent Contrast | Poor Contrast |
Vector® VIP (purple) | SK-4600 | Fair Contrast | Excellent Contrast | Poor Contrast |
ImmPACT® SG (blue-gray) | SK-4705 | Poor Contrast | Good Contrast | Excellent Contrast |
Vector® SG (blue-gray) | SK-4700 | Poor Contrast | Good Contrast | Excellent Contrast |
ImmPACT® NovaRED® (red) | SK-4805 | Excellent Contrast | Excellent Contrast *** | Color Incompatibility |
Vector® NovaRED® (red) | SK-4800 | Excellent Contrast | Excellent Contrast *** | Color Incompatibility |
ImmPACT® Vector® Red (magenta) | SK-5105 | Excellent Contrast | Excellent Contrast | Color Incompatibility |
Vector® Red (magenta) | SK-5100 | Excellent Contrast | Excellent Contrast | Color Incompatibility |
Vector® Black (black) | SK-5200 | Excellent Contrast | Excellent Contrast * | Excellent Contrast |
Vector® Blue (blue) | SK-5300 | Color Incompatibility | Good Contrast | Excellent Contrast |
BCIP/NBT (indigo) | SK-5400 | Color Incompatibility | Excellent Contrast * | Excellent Contrast |
* This substrate shows a slight decrease in sensitivity following the methyl green protocol. This decrease can be minimized by reducing the heat incubation and acetone rinse times in the methyl green protocol.
** Substrate dissolves in acetone wash.
*** A slight color change in ImmPACT NovaRED and Vector NovaRED reaction product may be seen using methyl green.
Product | Mountants | Catalog Number |
---|---|---|
Vector® Hematoxylin | Non-aqueous and Aqueous | H-3401-500 |
Vector® Hematoxylin QS | Non-aqueous and Aqueous | H-3404-100 |
Vector® Methyl Green | Non-aqueous | H-3402-500 |
Vector® Nuclear Fast Red | Non-aqueous and Aqueous | H-3403-500 |