Vector Laboratories is closed for the President’s Day on Monday, February 19th. We will be back in the office on Tuesday, February 20th.
We will respond to emails upon our return. Have a wonderful day.
Vector Methyl Green stains cell nuclei light green. It is an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors.
Features:
Unit Size | 500 ml |
---|---|
Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization |
Format | Ready-to-Use |
Methyl Green counterstain does take a little bit of optimization in some applications. We would suggest heating a volume of the counterstain (~300 ml) to 60 °C and add to a Coplin jar or similar glass staining rack, and submerse the slides. This approach facilitates a better uptake of stain into the section compared with placing the slide on a heated surface and placing drops of stain onto the section. Following the incubation time (~3-5 min), remove slides and wash in tap water. Omit the acetone, acetic acid rinse step described in the instructions and move the slides directly into 95% ethanol for the dehydration, clearing and mounting process. This methodology will retain more stain in the nuclei and hence produce greater intensity.
Counterstain/Substrate Compatibility Table
This table is designed as a reference to determine the optimal counterstain/substrate combination for your application.
Applicable patents and legal notices are available at legal notices.
Stay in the Loop. Join Our Online Community
We’re social scientists! Let’s connect.
Together we breakthroughTM
©Vector Laboratories, Inc. 2024 All Rights Reserved.
How do I Request a Quote?
To request a quote for products: