Brochures

Please find below free resources that will help you to optimize your assays. Please select one or more brochures from the list in the form, enter your contact information, and submit, and we will be happy to send you these resources free of charge. Otherwise, feel free to download and browse.

Immunohistochemistry Resource Guide

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.

IHC Multiplexing Guide

A valuable resource for those considering localizing two or more antigens in the same tissue section. Protocols for chromogenic detection are accompanied by schematic illustrations of the procedures. A chart of all pair-wise combinations of substrates is included along with photographic examples. Practical tips regarding order of substrates, blocking, and proper controls are also provided.

Immunofluorescence Resource Guide

Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents.  This guide will help you:

  • Learn about the IF workflow and how to optimize each step
  • Quickly identify and choose the most appropriate fluorescent secondary conjugates
  • Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving

Enzyme Substrates Brochure

In addition to photomicrographs which help the user visualize the color that is generally produced by each substrate, this brochure contains information on substrate properties especially useful for those who wish to multiplex (or introduce multiple labels in the same tissue section):  table of substrate properties, a schematic of relative senstitivities of substrate formulations, and a table of pairwise substrate compatibility.

Bioconjugation White Paper

Learn about quantitative and reproducible bioconjugation with the SoluLINK® technology platform which provides novel methods to quickly, easily and quantifiably link proteins, nucleic acids and other macromolecules to enzymes, fluorophores, haptens and other biomolecules. 

Automated IHC Protocols using ImmPRESS Polymer Reagents

We have produced an Application Note describing the use of our ImmPRESS HRP polymer on three popular autostainers: Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra. The Application Note includes complete staining protocols for each system and images of stained tissue sections obtained using the protocols.

Troubleshooting Guide for ABC Systems

A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol using ABC systems.  Also troubleshoot issues of weak or no staining. Laminated.

Troubleshooting Guide for Polymer Kits

A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol polymer detection systems.  Also troubleshoot issues of weak or no staining. Laminated.

Troubleshooting Mouse Primaries on Mouse Tissue

This guide for troubleshooting mouse primary antibodies on mouse tissue describes some common difficulties encountered in this procedure and details strategies for customizing the M.O.M. Kits to address them.

ImmPRESS® Polymer Detection Brochure

This ImmPRESS Enzyme Polymer brochure describes ImmPRESS micropolymer technology and includes schematics to help the user choose the most appropriate format for their application.

Lectins and Glycobiology Guide

This brochure contains a useful table of lectin properties summarizing biophysical information as well as sugar binding specificities and special applications for lectins available from Vector Labs. Also includes a complete product listing of all lectins, lectin conjugates and associated reagents.

Please Request Printed Copies with this Form