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SpeakEasy Science Blog

Adding efficiency and convenience to multiplexing workflows​

Multiplexing has revolutionized immunostaining workflows. It allows the simultaneous detection of multiple targets in the same tissue section and empowers scientists to gather more data while sparing precious samples. But issues with cross-reactivity between reagents can hinder the ability to perform simultaneous double or triple staining. When these issues arise, scientists can use the VectaPlexÔ Antibody Removal Kit to do multiple rounds of labeling and perform multiplexing experiments.

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Glycans and B-cells: How glycans influence adaptive immunity

The body’s autoimmune response has been leveraged by cancer researchers to propel immunotherapy tools into clinical use. Although the main focus has been on the ability of T-cells to fight cancer cells, the involvement of tumor-infiltrating B-cells is also becoming evident. Tumor-infiltrating B lymphocytes (TIL-Bs) are produced in much higher amounts in various cancers than in healthy tissues, which highlights their positive prognostic value (1). However, their exact role in cancer remains controversial, with the demonstration of both positive and negative impacts.

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Behind the Scenes: The Making of VectaPlex™ Antibody Removal Kit

Creating a product that stands out and addresses the needs of scientists requires ingenuity, persistence, and a lot of testing; our new product, VectaPlex™ Antibody Removal Kit, is no exception. We sat down with Senior Scientist August Estabrook, PhD, to get an inside look into the birth and development of VectaPlex. 

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Immunostaining basics: What to know before starting

Observation is one of the fundamental steps in the scientific method. However, for centuries the scientific study of tissues was limited to observations of dissections with the unaided eye. It was not until the mid-19th century with the introduction of dyes, such as hematoxylin, that the subcellular structure of tissues became visible and the science of histochemistry emerged (1). 

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How to use VectaPlex™ Antibody Removal Kit

Multiplexing, a powerful technique that allows the visualization of multiple biomarkers within a single sample, has revolutionized the field by offering researchers valuable insights into the spatial context of complex cellular processes and interactions. By analyzing multiple targets in the same specimen, researchers can obtain a holistic view of their tissue while conserving precious sample. However, performing multiplex assays can be challenging. Cross reactivity between detection reagents can often limit reagent choices and circumventing this by directly labeling primary antibodies can lead to altered antibody function and loss of specificity. Thankfully, VectaPlex™ Antibody Removal Kit comes to the rescue, simplifying the multiplex workflow, preserving tissue integrity, and enabling multiple rounds of staining without reducing target availability.

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All about VECTASTAIN® ABC Kits for immunodetection

Avidin-Biotin Complex, or ABC-based detection, is one of the most widely used methods for staining. This method exploits the high affinity exhibited between the protein avidin and the vitamin biotin forming large macromolecular complexes containing multiple enzyme molecules—either horse radish peroxidase (HRP) or alkaline phosphatase (AP).

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