Antibodies are the epitome of simple yet powerful science tools. Made of just 4 peptides, they never cease to help scientists unravel the intricacies of the microscopic world
Observation is one of the fundamental steps in the scientific method. However, for centuries the scientific study of tissues was limited to observations of dissections with the unaided eye. It was not until the mid-19th century with the introduction of dyes, such as hematoxylin, that the subcellular structure of tissues became visible and the science of histochemistry emerged (1).
Avidin-Biotin Complex, or ABC-based detection, is one of the most widely used methods for staining. This method exploits the high affinity exhibited between the protein avidin and the vitamin biotin forming large macromolecular complexes containing multiple enzyme molecules—either horse radish peroxidase (HRP) or alkaline phosphatase (AP).
In this blog post, we will explore IHC and IF techniques and how they have been used for identifying cancer biomarkers.
In this blog post, we will explore IHC and IF techniques and how they have been used for identifying cancer biomarkers.
Correctly coverslipping your slides ensures that your tissue specimens are preserved for optimal imaging and viewing. In this blog post, we will walk you through how to properly coverslip your slides to protect your samples.
With a wide variety to choose from, there are important considerations to make when selecting your own primary antibody. Keep reading to learn how to choose a primary antibody for your workflow.
As a part of Heart Health Awareness Month, we will be reviewing research being done to improve outcomes after Myocardial Infartion.
Rosa Fontana, PhD reflects on her journey in science, including leaving her home of Italy to discover new opportunities in the United States.
This blog covers important considerations for secondary antibody selection, including labeling method, species reactivity, antigen of interest location, optimal fluor conjugates, and signal amplification or multiple labeling (multiplexing) requirements.
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