Primary Antibodies for Immunofluorescence and Immunohistochemistry
Antibody specificity and sensitivity are two main factors that influence successful outcomes for immunofluorescence (IF) and immunohistochemistry (IHC) applications. Favorable antibody performance in other assays does not always translate to IF and IHC applications and can result in undesirable effects, including producing weak or no signal, showing nonspecific background staining that interferes with analysis, or even causing false positive signals.
Vector Laboratories’ 45+ years of experience in providing trusted, end-to-end workflow solutions is further supported by the addition of validated and highly cited primary antibodies for use in IF and IHC applications.
Recombinant Antibody Advantages
Reproducibility
High Purity
Supply Chain Security
Flexibility
Recombinant antibodies can be engineered into new formats, extending antibody usefulness and enabling new possibilities for IHC use.
Recombinant antibodies have emerged as a superior alternative to hybridoma-derived antibodies for immunohistochemistry offering enhanced performance and reliability. In a 2018 paper, Bradbury et al. randomly selected six different hybridoma derived monoclonal antibodies and their recombinant antibody counterparts to use in IHC analysis. The results showed that the recombinant antibodies had greater specificity and sensitivity than the hybridoma derived versions.
Absolute Antibody, now part of Vector Laboratories, has extensive expertise in recombinant antibody technology and offers an extensive catalog of recombinant antibodies for IF and IHC applications. The product portfolio has been curated to include unique antibody formats unavailable in any other reagents catalog. Using recombinant technology, antibody clones can be engineered into a variety of formats to enhance experimental flexibility. Custom antibody sequencing, engineering, and expression services are available for researchers wishing to expand the capabilities of their own antibodies.
Immunohistochemistry Validated Antibodies
LSBio, a Vector Laboratories brand, was established as a molecular pathology company with a focus on protein localization in diseased human tissues by IHC. Through the experience gained by executing thousands of IHC studies and testing many thousands of antibodies, LSBio curates and validates high-quality antibodies designed to support reliable performance in IHC and research pathology.
Primary Antibodies for Unique Targets and Specialized Applications
SCICONS® dsRNA Antibodies
SCICONS mouse anti-dsRNA antibodies are a highly cited offering used for the detection of dsRNA in immunofluorescence applications. These antibodies have been widely applied to study the viral life cycle and anti-viral responses, as well as used as a diagnostic tool to detect whether an unknown pathogen is bacterial or viral.
Cell Proliferation Antibodies
BrdU, or 5-bromo-2′-deoxyuridine, is a synthetic nucleoside analog that is commonly used in molecular biology and cell biology research to label newly synthesized DNA. BrdU detection antibodies and assay kits are available for streamlined non-isotopic analysis of cell proliferation.
Goat Polyclonal Antibodies
Our high-purity goat polyclonal antibodies for IHC and IF target a broad range of human, mouse, and rat proteins of biomedical interest. The specific high affinity and sensitivity of goat polyclonal antibodies backed by our stringent quality standards can improve assay quality, especially in samples with low target protein concentration.
Exclusive Institution-Derived Antibodies
Our portfolio includes a unique selection of antibodies developed by leading research institutions worldwide, providing the global scientific community with access to reagents not commercially available elsewhere. These institutionally derived antibodies have extensive publication records, highlighting their proven performance and reliability in specialized IF and IHC applications.
Endogenous alkaline phosphatase (AP) and peroxidase (HRP) activities in frozen intestine revealed with Vector® Red AP Substrate (red) and ImmPACT® DAB HRP Substrate (brown) (left). Same substrates used on BLOXALL Solution-treated tissue (right).