Antigen Unmasking
Unlock Epitope Accessibility for Optimal Immunostaining
Formalin fixation is a standard method in immunohistochemistry (IHC) and immunofluorescence (IF) workflows to preserve tissue morphology. However, formalin induces cross-linking of proteins which can mask antigenic sites and hinder antibody binding.
Antigen unmasking techniques restore epitope accessibility. The most common approach is heat-induced epitope retrieval (HIER) which involves heating tissue sections in specific buffer solutions to reverse formalin-induced cross-linking.
Vector Laboratories' Antigen Unmasking Solutions
To achieve optimal staining on formalin-fixed tissues in IHC and IF workflows, Vector Laboratories offers two highly effective antigen unmasking solutions, each supplied as a 100x concentrated stock for convenience and reproducibility.
When used in combination with high-temperature treatment, these solutions efficiently reverse formalin-induced cross-linking, exposing antigenic sites for improved antibody binding and enhanced staining intensity. The figure below demonstrates how IHC staining is enhanced by using Vector’s Tris-Based Antigen Unmasking Solutions (H-3301-250).
Two Formulations for Broad Antigen Coverage:
Vector’s antigen unmasking solutions are available in two formulations to address most experimental design needs.
Antigen Unmasking Solution, Citrate-Based (H-3300-250)
- pH: ~6.0
- Ideal for most common antigen retrieval applications
- Provides gentle but effective unmasking
- Compatible with a wide range of primary antibodies
Antigen Unmasking Solution, Tris-Based (H-3301-250)
- pH: ~9.0–10.5
- Designed for antigens requiring alkaline conditions
- Provides gentle but effective unmasking
- Compatible with a wide range of primary antibodies
Why Choose Vector Laboratories' Antigen Unmasking Reagents?
Convenient
Supplied as 100x concentrates allowing easy dilution for specific workflow needs.
Reproducible Results
Standardized formulations eliminate batch-to-batch variability, ensuring consistency across experiments.
Broad Compatibility
Two formulation options cover the majority of primary antibody retrieval requirements.
Seamless Integration
Designed to work efficiently with Vector’s IHC and IF detection systems.
Antigen Unmasking Technical and Educational Resources
For more information about antigen unmasking and how Vector products can streamline this step in your IHC and IF workflows, explore our resources.
Staining Sample Preparation Guide
