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Species-On-Species

Sensitivity. Reliability. Versatility.

Species-on-species detection, such as mouse-on-mouse and human-on-human immunodetection workflows, can pose significant challenges due to high background staining and cross-reactivity with endogenous immunoglobulins. Conventional detection methods often struggle to differentiate between primary antibodies and native immunoglobulins in tissue samples, leading to non-specific staining and ambiguous results.

Vector Laboratories offers specialized immunodetection kits designed to eliminate these obstacles and enable clear, specific staining of primary antibodies in challenging species-on-species applications.

Mouse-on-Mouse (M.O.M.®) Immunodetection Kit

The Vector M.O.M. (Mouse-on-Mouse) Immunodetection Kits are engineered to address the challenge of high background staining when detecting mouse antigens in mouse tissues. Vector M.O.M. Kits offer a streamlined workflow with no complex calculations or pre-binding steps, making what was once a difficult process now simple and routine. The result? Clear, crisp, and specific antigen detection in mouse tissue samples. Ideal for use in studies involving genetically engineered mice, transgenic models, knock-out models, and xenografts.

Without M.O.M.
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Without M.O.M. Mouse intestine stained with standard anti-mouse Ig polymer system and Vector DAB Substrate. Hematoxylin counterstain. Note background IgG staining
With M.O.M.
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With M.O.M. Mouse intestine stained with Vector M.O.M. ImmPRESS® Kit, Vector DAB, and no primary antibody.

Available M.O.M Immunodetection Kits

BMK-2202 

M.O.M.® (Mouse on Mouse) Immunodetection Kit, Basic   

MP-2400 

M.O.M.® (Mouse on Mouse) ImmPRESS® HRP (Peroxidase) Polymer Kit  

PK-2200 

M.O.M.® (Mouse on Mouse ) Elite® Immunodetection Kit, Peroxidase

FMK-2201 

M.O.M.® (Mouse on Mouse) Immunodetection Kit, Fluorescein

MKB-2213-1

M.O.M.® (Mouse on Mouse) Blocking Reagent

MKB-2225-.1 

M.O.M.® (Mouse on Mouse) Biotinylated Anti-Mouse IgG Reagent

MPX-2402-15 

M.O.M.® (Mouse on Mouse) ImmPRESS® Polymer Reagent, Anti-Mouse IgG, Peroxidase 

Human-on-Human (H.O.H.â„¢) Immunodetection Kit

The Vector H.O.H. (Human-on-Human) Immunodetection Kit is designed for immunohistochemical detection of human antigens in human tissue sections. By incorporating proprietary blocking technology, the H.O.H. Kit ensures precise and specific antigen detection, even in complex human tissue samples. This system is ideal for pathology research, clinical studies, and biomarker discovery, where accurate immunohistochemical analysis of human tissues is critical.

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 Serial sections of human kidney (FFPE) showing strong, specific staining (brown regions) using human anti-cytokeratin primary antibody detected with HOH-3000. 
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Negative control. Serial sections of human kidney (FFPE) showing an absence of staining (no background) with the omission of the primary antibody in the HOH-3000 assay. Note: Both sections counterstained with hematoxylin (blue nuclei).

Humanized anti-Desmin on frozen human colon

Standard IHC Detection
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Vector H.O.H. Detection
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Humanized anti-Cytokeratin on FFPE human kidney

Standard IHC Detection
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Vector H.O.H. Detection
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Available H.O.H Immunodetection Kits

HOH-3000 

H.O.H.® (Human on Human) Immunodetection Kit 

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Technical and Educational Resources

View our resources below for more in-depth immunofluorescence and immunohistochemistry information.

Immunofluorescence Resource Guide

Streamline the immunofluorescence process with this step-by-step guide to finding the optimal reagents for your project.

Immunohistochemistry Resource Guide

Guide Step-by-step guide for selecting the best reagents for your immunohistochemistry workflow.

IHC Multiplexing Resource Guide

Discover the steps and considerations needed for creating stunning multicolor IHC images.

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