Blocking Action Endogenous Mouse Ig | |
Format Concentrate | |
Unit Size 1 ml | |
Storage Instructions 2-8 °C | |
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications |
M.O.M. (Mouse on Mouse) Blocking Reagent
Mouse on Mouse (M.O.M.) Blocking Reagent is used to block endogenous mouse immunoglobulins in a mouse tissue section and is a key component of the M.O.M. Kits.
Features of the M.O.M. Immunodetection system:
• Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
• Clear, crisp specific staining of antigens of interest
• Based on VECTASTAIN® ABC or ImmPRESS® reagent protocols
• Procedures are simple and easy to follow
• No tedious calculations or antibody prebinding steps required
$302.00
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Technical Information The Vector® M.O.M. Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector M.O.M. Kit. The result is clear, crisp specific staining of the antigens of interest. Excellent staining results for a once difficult application have now become routine with the Vector M.O.M. System. The Vector M.O.M. Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts. This reagent is supplied as a 1 ml concentrate, sufficient to prepare about 25 ml of working solution or enough to stain about 125-250 sections. The M.O.M. Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems. Detailed information for multiple antigen labeling can be found in our brochure, “Discovery Through Color: A Guide to Multiple Antigen Labeling” , available on our website. Four Vector M.O.M. Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M. Mouse Ig Blocking Reagent and the M.O.M. Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M. Mouse Ig Blocking Reagent and M.O.M. Biotinylated Anti-Mouse Ig Reagent are also available separately. The Vector M.O.M. ImmPRESS Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M. ImmPRESS Peroxidase Polymer reagent. Optimal staining results are obtained when this reagent is paired with the special M.O.M. Biotinylated Anti-Mouse Ig Reagent or the biotin-free, one-step micropolymer detection reagent, ImmPRESS Anti-Mouse Ig Reagent (MPX-2402). |
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Product FAQs
What is the protocol for using the individual M.O.M.® (Mouse on Mouse) Blocking Reagent (MKB-2213-1)?
The working solution of the M.O.M. Blocking Reagent can be prepared by adding 2 drops (~90 µL) of M.O.M. Blocking Reagent stock solution to 2.5 mL of PBS or TBS (PBS: 10 mM sodium phosphate, 0.15 M NaCl, pH 7.4-7.8; TBS: 50 mM TRIS, 0.15 M NaCl, pH 7.5-7.8). Incubate sample for 1 hour at room temperature in working solution of prepared M.O.M. Blocking Reagent to block endogenous mouse immunoglobulins in mouse tissue. This step must be completed prior to the incubation with the primary antibody.
