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VectaMount® Express Mounting Medium (H-5700-60)

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Description

VectaMount® Express is a non-aqueous clearing and mounting medium enabling the rapid mounting of cell and tissue specimens following immunohistochemistry (IHC) staining. This novel formulation is engineered to enable mounting directly following staining, saving time by eliminating the need for extensive ethanol and clearing washes prior to coverslipping. Just stain your slides as per your usual workflow, briefly wash in isopropyl alcohol, and coverslip. 

Features:

  • Non-aqueous clearing and mounting medium for IHC-stained slides
  • Eliminates the need for extensive ethanol washes and solvent-based clearing agents (e.g., xylene)
  • Rapid drying formula for fast visualization of stained samples
  • Preserves staining for at least 18 months at room temperature
  • Compatible with horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme substrates, as well as commonly used counterstains
  • Optically clear formula with a refractive index of 1.49, resulting in high-quality imaging free of distortion or artifact

Specifications

Unit Size60 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended UsageVectaMount Express Mounting Medium is a non-aqueous clearing and mounting medium enabling the rapid mounting of cell and tissue specimens following IHC staining. VectaMount Express is compatible with HRP and AP enzyme substrates, as well as commonly used counterstains.
Recommended StorageRoom Temperature
MountingNon-Aqueous
AntifadeNo
Safety TitleWARNING: Reproductive Harm – www.P65Warnings.ca.gov

Product FAQs

It is important to use 99+% alcohol (isopropanol or ethanol) for the brief dehydration steps. Lower alcohol grades will not be viable substitutes. Slides should be rinsed in tap water (not buffer) prior to dehydration, as salts in buffers can precipitate if not removed. Depending on slide volume, alcohol baths should be changed out on a regular basis with fresh solution. It is suggested to drain excess water when transferring slides from tap water into the alcohol bath.

Usually the presence of air bubbles can be greatly reduced, and even eliminated, when using a manual approach, by carefully lowering the coverslip slowly over the specimen. Sometimes this may take a little practice to perfect.

No. It is recommended to dry slides at ambient room temperature.

There are no specific time limits for slides to be in the alcohol bath. Depending on the number of slides in a given assay, the last slide to be mounted will be in the alcohol bath longer than the first mounted slide. This may take several minutes or much longer. We have not seen any observable difference in specimen characteristics, staining or media performance with longer alcohol exposure times.

Place the slides in a glass staining rack or Coplin jar and completely submerge the slides in xylene overnight. After this time the coverslip should be easy to remove from the slide.

Yes. From our in-house testing, commonly used counterstains such as hematoxylin (e.g. Gill’s and Mayers formulations), nuclear fast red, methyl green and standard H&E (hematoxylin and eosin) stains are compatible with VectaMount Express Mounting Medium.

Technical Information

VectaMount Express Mounting Medium is a non-aqueous clearing and mounting medium enabling the rapid mounting of cell and tissue specimens, without the need for slide handling through an ethanol dehydration series and xylene clearing prior to coverslipping. VectaMount Express Mounting Medium preserves the color and intensity of preparations stained with enzyme substrates, such as DAB and BCIP/NBT, as well as our proprietary substrates Vector® NovaRED , Vector VIP, Vector SG, ImmPACT® DAB, ImmPACT DAB EqV, ImmPACT NovaRED, ImmPACT VIP, ImmPACT SG, Vector Red, ImmPACT Vector Red, and Vector Blue, for at least 18 months at room temperature. With a refractive index (1.49) closely matching that of the slide and tissue specimen, this mounting medium results in high-quality imaging free of distortion or artifact.

Preserves staining for at least 18 months

 ImmDAB VME Colon v2

Human Colon: Mouse Anti-Cytokeratin (AE1/AE3), ImmPRESS-HRP Anti-Mouse IgG, ImmPACT DAB Mounted in VectaMount Express Mounting Media and imaged after 18 months of storage.

ImmDAB VME Colon v1

Human Colon: Mouse Anti-Cytokeratin (AE1/AE3), ImmPRESS-HRP Anti-Mouse IgG, ImmPACT DAB Mounted in VectaMount Express Mounting Media and imaged after 18 months of storage.

DAB Ni VME Colon v1

Human Colon: Mouse Anti-Cytokeratin (AE1/AE3), ImmPRESS-HRP Anti-Mouse IgG, DAB-Ni Mounted in VectaMount Express Mounting Media and imaged after 18 months of storage.

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