Protocols

Selected Vector Laboratories kit instructions and associated protocols are listed below in PDF format. If you are unable to obtain the desired information, please contact Vector Laboratories technical service or your local Vector Laboratories distributor for further assistance. 

VECTASTAIN® Kits

VECTASTAIN® Elite ABC-Peroxidase Kit (included in all PK-6100 series kits)
VECTASTAIN® Universal Elite ABC-Peroxidase Kit (PK-6200)
R.T.U. VECTASTAIN® Elite ABC-Peroxidase Reagent (PK-7100)
R.T.U. VECTASTAIN® Universal Elite ABC-Peroxidase Kit (PK-7200)
VECTASTAIN® ABC-Peroxidase Kit (included in all PK-4000 series kits)
VECTASTAIN® ABC-Alkaline Phosphatase Kit (included in all AK-5000 series kits)
VECTASTAIN® Universal ABC-Alkaline Phosphatase Kit (AK-5200)
VECTASTAIN® Universal Quick Kit (PK-8800)
R.T.U. VECTASTAIN® Universal Quick Kit (PK-7800)

Supplemental Protocols for Vector® Kits and Reagents

Enzyme Immunoassays, Hybridoma Screening, and Western Blots
BLOXALL™ Solution for Quenching Endogenous Peroxidase and Alkaline Phosphatase Activity
Quenching of Endogenous Peroxidase Activity
Recommended Dilutions and Buffers
Troubleshooting Guide
Enzyme Substrate Compatibility Chart

ImmPRESS™ Polymerized Reporter Enzyme Staining System

ImmPRESS™-AP Anti-Rabbit Ig (alkaline phosphatase) (MP-5401)
ImmPRESS™-AP Anti-Mouse Ig (alkaline phosphatase) (MP-5402)
Vector® Mouse on Mouse (M.O.M.™) ImmPRESS Peroxidase Polymer Immunodetection Kit (MP-2400)
ImmPRESS™ Anti-Rabbit Ig (peroxidase) (MP-7401)
ImmPRESS™ Anti-Mouse Ig (peroxidase) (MP-7402)
ImmPRESS™ Anti-Mouse Ig, Rat-adsorbed (peroxidase) (MP-7422)
ImmPRESS™ Anti-Rat Ig (peroxidase) (MP-7404)
ImmPRESS™ Anti-Rat Ig, Mouse-adsorbed (peroxidase) (MP-7444)
ImmPRESS™ Anti-Goat Ig (peroxidase) (MP-7405)
ImmPRESS™ Universal Antibody Kit (peroxidase) (MP-7500)
ImmPRESS™ Excel Anti-Rabbit Ig (peroxidase, complete) (MP-7601)
ImmPRESS™ Excel Anti-Mouse Ig (peroxidase, complete) (MP-7602)

Vector Mouse on Mouse (M.O.M.™) Immunodetection Kit

Vector® Mouse on Mouse (M.O.M.™) Elite Peroxidase Immunodetection Kit (PK-2200)
Vector® Mouse on Mouse (M.O.M.™) Fluorescein Immunodetection Kit (FMK-2201)
Vector® Mouse on Mouse (M.O.M.™) Basic Immunodetection Kit (BMK-2202)
Multiple Immunofluorescent Staining Using Mouse Antibodies
Troubleshooting Mouse Primaries on Mouse Tissue
Multiple Antigen Labeling in the Same Tissue Sections
Vector® Mouse on Mouse (M.O.M.™) ImmPRESS Peroxidase Polymer Immunodetection Kit (MP-2400)

Counterstains

Vector® Hematoxylin (H-3401)
Vector® Hematoxylin QS (H-3404)
Vector® Methyl Green (H-3402)
Vector® Nuclear Fast Red (H-3403)
Counterstain/Substrate Compatibility Chart

Mounting Media

VectaMount™ Permanent Mounting Medium (H-5000)
VectaMount™ AQ Aqueous Mounting Medium (H-5501)
VECTASHIELD® Mounting Media (H-1000)
VECTASHIELD® Mounting Media with DAPI (H-1200)
VECTASHIELD® Mounting Media with PI (H-1300)
VECTASHIELD® HardSet Mounting Media (H-1400)
VECTASHIELD® HardSet Mounting Media with DAPI (H-1500)
VECTASHIELD® Vibrance Antifade Mounting Medium (H-1700)
VECTASHIELD® Vibrance Antifade Mounting Medium with DAPI (H-1800)

Immunofluorescence

Double Immunofluorescent Labeling
VectaFluor™ DyLight® 488 Anti-Goat Ig (DI-3788)
VectaFluor™ DyLight® 488 Anti-Mouse Ig (DI-2788)
VectaFluor™ DyLight® 488 Anti-Rabbit Ig (DI-1788)
VectaFluor™ DyLight® 594 Anti-Goat Ig (DI-3794)
VectaFluor™ DyLight® 594 Anti-Mouse Ig (DI-2794)
VectaFluor™ DyLight® 594 Anti-Rabbit Ig (DI-1794)
VectaFluor™ Excel Amplified DyLight® 488 Anti-Mouse Ig (DK-2488)
VectaFluor™ Excel Amplified DyLight® 488 Anti-Rabbit Ig (DK-1488)
VectaFluor™ Excel Amplified DyLight® 594 Anti-Mouse Ig (DK-2594)
VectaFluor™ Excel Amplified DyLight® 594 Anti-Rabbit Ig (DK-1594)
VECTABOND™ Reagent for Tissue Section Adhesion (SP-1800)
Antigen Unmasking Solution (H-3300, H-3301)
Avidin/Biotin Blocking Kit (SP-2001)
Streptavidin/Biotin Blocking Kit (SP-2002)
TrueVIEW Autofluorescence Quenching Kit (SP-8400)

Enzyme Substrates

ImmPACT™ DAB Substrate Kit (SK-4105)
ImmPACT™ DAB EqV Substrate Kit (SK-4103)
ImmPACT™ AEC Substrate Kit (SK-4205)
ImmPACT™ AMEC Red Substrate Kit (SK-4285)
ImmPACT™ VIP Substrate Kit (SK-4605)
ImmPACT™ SG Substrate Kit (SK-4705)
ImmPACT™ NovaRED Substrate Kit (SK-4805)
DAB Substrate Kit (SK-4100)
DAB Enhancing Solution (H-2200)
Vector® VIP Substrate Kit (SK-4600)
Vector® SG Substrate Kit (SK-4700)
Vector® NovaRED Substrate Kit (SK-4800)
AEC Substrate Kit (SK4200)
TMB Substrate Kit (SK-4400)
DuoLuX™ Chemiluminescent/Fluorescent Substrate Kit for peroxidase (SK-6604)
ImmPACT™ Vector® Red Substrate Kit (SK-5105)
Vector® Red Substrate Kit (SK-5100)
Vector® Blue Substrate Kit (SK-5300)
Vector® Black Substrate Kit (SK-5200)
BCIP/NBT Substrate Kit (SK-5400)
Levamisole Solution (SP-5000)
p-Nitrophenyl phosphate Substrate Kit (SK-5900)
DuoLuX™ Chemiluminescent/Fluorescent Substrate Kit for alkaline phosphatase (SK-6605)

Blot Detection Systems

WestVision™ Peroxidase Polymer Reagents for Western Blotting (WB-1000, WB-2000)
VECTASTAIN® ABC-AmP™ Western Blot Detection System (AK-6000 series kits)
Troubleshooting Guide for VECTASTAIN® ABC-AmP™ Kits

In Situ Hybridization

Fluorescence Detection of Biotin-Labeled ISH Probes
Fluorescence Detection of Fluorescein-Labeled ISH Probes
Chromogenic Detection of Biotin-Labeled ISH Probes
Chromogenic Detection of Fluorescein-Labeled ISH Probes
In Situ Hybridization Detection Systems (General guidelines)
Amplification of FISH using Biotinylated Anti-Avidin (BA-0300)

Biotin Labeling Reagents

Biotin (Long Arm) NHS (SP-1200)
Biotin (Long Arm) NHS, water soluble (SP-1210)
Biotin (Long Arm) Maleimide (SP-1501)
Biotin (Long Arm) Hydrazide (SP-1100)

Biotin Quantitation Kit

Quant*Tag Biotin Kit (BDK-2000)

Thiol-Reactive Labeling Reagents

Biotin Maleimide (SP-1501)
Fluorescein Maleimide (SP-1502)
Texas Red Maleimide (SP-1505)

Molecular Biology Reagents

5' EndTag Labeling System (MB-9001)
3' EndTag Labeling System (MB-9002)
PHOTOPROBE® Biotin (SP-1000, SP-1020)
Detection of Labeled Nucleic Acid Probes on Blots
Spin Column Chromatography
In Situ Hybridization Detection Systems
Amplification of FISH using Biotinylated Anti-Avidin (BA-0300)

Neuronal Tracing reagents

PHA-L (L-1110)
NEUROBIOTIN™ Tracer (SP-1120)

Lectins

Lectins in Histochemistry, ELISA, and Western Blot Applications
Peroxidase localization of WGA in Neural Tissue (L-1020)
Agarose WGA - Use and Regeneration
Enrichment of NK cells by panning on ECL plates (L-1140)
Specificity of Jacalin binding (L-1150)