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Protocols

Selected Vector kit instructions and associated protocols are listed below in PDF format. If you are unable to obtain the desired information please contact Vector technical service or your local Vector Labs distributor for further assistance.

Supplemental Protocols for Vector® Kits and Reagents

Human on Human Immunodetection Kit (User Guide) (HOH-3000)
Antigen Unmasking Solution (Tris-based, pH 9.0) (User Guide) (H-3301-250)
Enzyme Immunoassays, Hybridoma Screening, and Western Blots
BLOXALL® Solution for Quenching Endogenous Peroxidase and Alkaline Phosphatase Activity (SP-6000-100)
Recommended Dilutions and Buffers
Antigen Unmasking Solution (Citrate-based, pH 6.0) (User Guide) (H-3300-250)

Biotin Labeling Reagents

PHOTOPROBE ® Biotin (User Guide) (SP-1000-.5,SP-1020-.5)
Biotin (Long Arm) NHS (SP-1200-50)
Biotin (Long Arm) NHS, water soluble (SP-1210-50)

VECTASTAIN® Kits

VECTASTAIN Elite ABC Universal PLUS Kit User Guide (PK-8200)
VECTASTAIN Elite ABC-Peroxidase Kit (User Guide) (PK-6100,PK-6101,PK-6102,PK-6103,PK-6104,PK-6105,PK-6106)
VECTASTAIN Universal Elite ABC-Peroxidase Kit (User Guide) (PK-6200)
R.T.U. VECTASTAIN EliteABC-Peroxidase Reagent (User Guide) (PK-7100)
R.T.U. VECTASTAIN Universal Elite ABC-Peroxidase Kit (User Guide) (PK-7200)
VECTASTAIN ABC-Peroxidase Kit (User Guide) (PK-4000,PK-4001,PK-4002,PK-4003,PK-4004,PK-4005,PK-4006,PK-4007,PK-4010)
VECTASTAIN ABC-Alkaline Phosphatase Kit (User Guide) (AK-5000,AK-5001,AK-5002,AK-5003,AK-5004,AK-5005,AK-5006,AK-5007,AK-5010)
VECTASTAIN Universal ABC-Alkaline Phosphatase Kit (User Guide) (AK-5200)
VECTASTAIN Universal Quick Kit (User Guide) (PK-8800)
R.T.U. VECTASTAIN Universal Quick Kit (User Guide) (PK-7800)

Bioconjugation Reagents

Oligonucleotide Concentration Determination
B-9007-105 User Guide/Protocol (B-9007-105K,B-9007-105)
Using a NanoDrop to Measure Antibody Concentration (A-9002,A-9002-001)
A-9002-001 User Guide/Protocol (A-9002)
A-9202-001 User Guide/Protocol (A-9202)
B-1001 User Guide/Protocol (B-1001)
B-1007 User Guide/Protocol (B-1007)
B-1012 User Guide/Protocol (B-1012)
B-9007-009 User Guide/Protocol (B-9007)
B-9014 User Guide/Protocol (B-9014)
M-1002 User Guide/Protocol (M-1002)
M-1003 User Guide/Protocol (M-1003)
S-1001 User Guide/Protocol (S-1001)
S-1002 User Guide/Protocol (S-1002)
S-1004 User Guide/Protocol (S-1004)
S-1008 User Guide/Protocol (S-1008)
S-1009 User Guide/Protocol (S-1009)
S-1011 User Guide/Protocol (S-1011)
S-1037 User Guide/Protocol (S-1037)
S-9010 User Guide/Protocol (S-9010)
S-9011 User Guide/Protocol (S-9011)
HyNic Protein MSR Instructions (S-1001,S-1001-010,S-1002,S-1002-105,S-1002-010,S-1009,S-1009-010,S-1011,S-1011-105,S-1011-010)
Sample Protocols for Streptavidin Agarose Ultra Performance (N-1000,N-1000-002,N-1000-005,N-1000-010)
P-9002 User Guide/Protocol (P-9002)
Troubleshooting Guide – Bioconjugation (A-9002,A-9002-001,A-9202,A-9202-001,B-1001,B-1001-105,B-1001-010,B-1007,B-1007-105,B-1007-110,B-1012,B-1012-010,B-9007,B-9007-105K,B-9007-009K,B-9007-105,B-9014,B-9014-009K,P-9002,P-9002-002,S-1002,S-1002-105,S-1002-010,S-1004,S-1004-105,S-1004-010,S-1008,S-1008-105,S-1008-010,S-1009,S-1009-010,S-1011,S-1011-105,S-1011-010,S-9010,S-9010-1,S-9011,S-9011-1)
4FB Oligonucleotide MSR Instructions (S-1004,S-1004-105,S-1004-010,S-1008,S-1008-105,S-1008-010)
4FB Protein MSR Instructions (S-1004,S-1004-105,S-1004-010,S-1008,S-1008-105,S-1008-010)
Concentration of Dilute Antibody Solutions (A-9002,A-9002-001,A-9202,A-9202-001)
HyNic Protein MSR Instructions (S-1002,S-1002-105,S-1002-010,S-1009,S-1009-010,S-1011,S-1011-105,S-1011-010)
Using a NanoDrop to Measure Antibody Concentration (A-9002,A-9002-001)
Oligonucleotide Buffer Exchange and Desalting Protocol (B-1001,B-1001-105,B-1001-010,B-1007,B-1007-105,B-1007-110,B-1012,B-1012-010,S-1002,S-1002-105,S-1002-010,S-1004,S-1004-105,S-1004-010,S-1008,S-1008-105,S-1008-010,S-1009,S-1009-010)
Protein Buffer Exchange and Desalting Protocol (B-1001,B-1001-105,B-1001-010,B-1007,B-1007-105,B-1007-110,B-1012,B-1012-010,S-1002,S-1002-105,S-1002-010,S-1004,S-1004-105,S-1004-010,S-1008,S-1008-105,S-1008-010,S-1009,S-1009-010)
BCA Protein Assay Protocol (A-9002,A-9002-001,B-1001,B-1001-105,B-1001-010,B-1007,B-1007-105,B-1007-110,B-1012,B-1012-010,P-9002,P-9002-002,S-1002,S-1002-105,S-1002-010,S-1004,S-1004-105,S-1004-010,S-1008,S-1008-105,S-1008-010,S-1009,S-1009-010,S-1011,S-1011-105,S-1011-010,S-9010,S-9010-1,S-9011,S-9011-1)
Bradford Assay Protocol (A-9002,A-9002-001,B-1001,B-1001-105,B-1001-010,B-1007,B-1007-105,B-1007-110,B-1012,B-1012-010,P-9002,P-9002-002,S-1002,S-1002-105,S-1002-010,S-1004,S-1004-105,S-1004-010,S-1008,S-1008-105,S-1008-010,S-1009,S-1009-010,S-1011,S-1011-105,S-1011-010,S-9010,S-9010-1,S-9011,S-9011-1)

Immunofluorescence

VectaFluor™ Duet Double Labeling Kit (User Guide) (DK-8818)
VectaFluor™ Duet Double Labeling Kit (User Guide) (DK-8828)
TrueVIEW® Autofluorescence Quenching Kit (User Guide) (SP-8400-15)
TrueVIEW® Autofluorescence Quenching Kit with DAPI (User Guide) (SP-8500-15)
Double Immunofluorescent Labeling Protocol (A-2011-1,A-2016-1)
VectaFluor™ DyLight™ 488 Anti-Rabbit Ig (DI-1788-15)
VectaFluor™ DyLight™ 594 Anti-Mouse Ig (DI-2794-15)
VectaFluor™ DyLight™ 594 Anti-Rabbit Ig (DI-1794-15)
VectaFluor™ Excel Amplified DyLight™ 488 Anti-Mouse Ig (DK-2488)
VectaFluor™ Excel Amplified DyLight™ 488 Anti-Rabbit Ig (DK-1488)
VectaFluor™ Excel Amplified DyLight™ 594 Anti-Mouse Ig (DK-2594)
VectaFluor™ Excel Amplified DyLight™ 594 Anti-Rabbit Ig (DK-1594)
VECTABOND® Reagent for Tissue Section Adhesion (SP-1800-7)
Avidin/Biotin Blocking Kit (SP-2001)
Streptavidin/Biotin Blocking Kit (SP-2002)

NEUROBIOTIN 488 Tracer (SP-1125)
Biotin (Long Arm) Hydrazide (SP-1100) (SP-1100-50)
VectaCell Trolox Antifade Reagent (CB-1000) (CB-1000-2)
WestVision™ Block and Diluent for Western Blots (SP-7000) (SP-7000-500)
5’ EndTag Nucleic Acid End Labeling System (MB-9001) (MB-9001)
NEUROBIOTIN 488 Tracer (SP-1125) (SP-1125-2)

ImmPRESS® Polymerized Reporter Enzyme Staining System

ImmPRESS Duet Double Staining HRP/AP Polymer Kit (User Guide) (MP-7714-15)
ImmPRESS Duet Double Staining HRP/AP Polymer Kit (User Guide) (MP-7724-15)
ImmPRESS HRP Universal PLUS Polymer Kit (User Guide) (MP-7800-15)
ImmPRESS HRP Horse Anti-Rabbit IgG PLUS Polymer Kit (User Guide) (MP-7801-15)
ImmPRESS HRP Horse Anti-Mouse IgG PLUS Polymer Kit (User Guide) (MP-7802-15)
ImmPRESS-VR HRP Horse Anti-Rabbit IgG Polymer (User Guide) (MP-6401-15)
ImmPRESS-VR HRP Horse Anti-Mouse IgG Polymer Kit (User Guide) (MP-6402-15)
ImmPRESS HRP Goat Anti-Rabbit IgG Polymer Kit (User Guide) (MP-7451)
ImmPRESS HRP Goat Anti-Mouse IgG Polymer Kit (User Guide) (MP-7452)
ImmPRESS-AP Goat Anti-Rat IgG Polymer Kit (User Guide) (MP-5404-15)
ImmPRESS-AP Horse Anti-Goat IgG Polymer Kit (User Guide) (MP-5405)
ImmPRESS-AP Goat Anti-Rat IgG (Mouse Adsorbed) Polymer Kit (User Guide) (MP-5444-15)
ImmPRESS-AP Horse Anti-Rabbit IgG Polymer Kit (User Guide) (MP-5401)
ImmPRESS-AP Horse Anti-Mouse IgG Polymer Kit (User Guide) (MP-5402)
M.O.M.® (Mouse on Mouse) ImmPRESS Peroxidase Polymer Kit (User Guide) (MP-2400)
ImmPRESS HRP Horse Anti-Rabbit IgG Polymer Kit (User Guide) (MP-7401)
ImmPRESS HRP Horse Anti-Mouse IgG Polymer Kit (User Guide) (MP-7402)
ImmPRESS HRP Horse Anti-Mouse IgG, Rat-adsorbed Polymer Kit (User Guide) (MP-7422-15)
ImmPRESS HRP Goat Anti-Rat IgG Polymer Kit (User Guide) (MP-7404-50)
ImmPRESS HRP Goat Anti-Rat IgG, Mouse-adsorbed Polymer Kit (User Guide) (MP-7444-15)
ImmPRESS HRP Horse Anti-Goat IgG Polymer Kit (User Guide) (MP-7405)
ImmPRESS HRP Universal Polymer Kit (User Guide) (MP-7500)
ImmPRESS Excel Amplified Polymer Kit, Anti-Rabbit IgG (User Guide) (MP-7601)
ImmPRESS Excel Amplified HRP Polymer Kit, Anti-Mouse IgG (User Guide) (MP-7602)

Lectins

Glysite™ Scout Glycan Screening Kit 649 User Guide (GSK-1000)
Glysite™ Scout Glycan Screening Kit 594 User Guide (GSK-2000)
Glysite™ Scout Glycan Screening Kit 488 User Guide (GSK-3000)
Lectins in Histochemistry, ELISA, and Western Blot Applications (AL-1013-2,AL-1063-2,AL-1143-2,AL-1173-2,AL-1183-2,AL-1233-2,AL-1303-2,AL-1353-2,AL-1393-2,B-1005-5,B-1005S-5,B-1015-5,B-1025-5,B-1025S-5,B-1035-5,B-1045-5,B-1055-5,B-1065-2,B-1075-5,B-1085-5,B-1105-2,B-1115-2,B-1125-2,B-1145-5,B-1155-5,B-1165-2,B-1175-1,B-1185-2,B-1205-.5,B-1215-2,B-1235-2,B-1245-2,B-1265-1,B-1305-2,B-1315-2,B-1325-2,B-1355-2,B-1375-2,B-1385-2,B-1395-1,BK-1000,BK-3000,DL-1174-1,DL-1177-1,DL-1207-.5,FL-1001-25,FL-1011-2,FL-1021S-5,FL-1041-5,FL-1081-5,FL-1111-2,FL-1121-2,FL-1141-5,FL-1151-5,FL-1161-2,FL-1171-1,FL-1181-2,FL-1201-.5,FL-1211-2,FL-1231-2,FL-1241-2,FL-1301-2,FL-1311-2,FL-1321-2,FL-1341-2,FL-1351-2,FL-1391-1,FLK-2100,FLK-4100,L-1000-500,L-1050-10,L-1080-10,L-1090-10,L-1100-5,L-1110-5,L-1120-5,L-1140-10,L-1160-5,L-1170-2,L-1180-5,L-1190-1,L-1210-5,L-1230-5,L-1240-5,L-1260-2,L-1280-5,L-1290-1,L-1300-5,L-1310-5,L-1320-5,L-1340-5,L-1350-5,L-1370-5,L-1390-2,L-1104-1,L-1410-5,DL-1178-1,AL-1003,AL-1023,AL-1023S,AL-1043,AL-1073,AL-1083,AL-1153,AL-1243,FL-1021,FL-1031,FL-1061,FL-1071,FL-1101,L-1010,L-1020,L-1030,L-1040,L-1060,L-1070,L-1150,DL-1048-1,L-1420-2,DL-1208-.5,DL-1067-1,DL-1068-1,CL-1003-1,CL-1073-1,CL-1075-1,CL-1303-1,CL-1305-1,BK-2100)
Peroxidase localization of WGA in Neural Tissue (L-1020)
Agarose WGA - Use and Regeneration (AL-1023)
Enrichment of NK cells by panning on ECL plates (L-1140-10)
Specificity of Jacalin binding (L-1150)

Counterstains

Alcian Blue (pH 2.5) Stain Kit (User Guide) (H-3501)
Hematoxylin & Eosin Stain Kit (User Guide) (H-3502)
Vector® Hematoxylin (User Guide) (H-3401-500)
Vector® Hematoxylin QS (User Guide) (H-3404-100)
Vector® Methyl Green (User Guide) (H-3402-500)
Vector® Nuclear Fast Red (User Guide) (H-3403-500)

Mounting Media

VECTASHIELD® HardSet Antifade Mounting Medium with Phalloidin (User Guide) (H-1600-10)
VECTASHIELD PLUS Antifade Mounting Medium (User Guide) (H-1900)
VECTASHIELD PLUS Antifade Mounting Medium with DAPI (User Guide) (H-2000)
VECTASHIELD® Vibrance Antifade Mounting Medium (User Guide) (H-1700)
VECTASHIELD® Vibrance Antifade Mounting Medium with DAPI (User Guide) (H-1800)
VectaMount® Permanent Mounting Medium (H-5000-60)
VectaMount® AQ Aqueous Mounting Medium (H-5501-60)
VECTASHIELD® Mounting Media (User Guide) (H-1000-10)
VECTASHIELD® Mounting Media with DAPI (User Guide) (H-1200-10)
VECTASHIELD®Mounting Media with PI (User Guide) (H-1300-10)
VECTASHIELD®HardSet Mounting Media (User Guide) (H-1400-10)
VECTASHIELD®HardSet Mounting Media with DAPI (User Guide) (H-1500-10)

Vector Mouse on Mouse (M.O.M.®) Immunodetection Kit

M.O.M. (Mouse on Mouse) Elite Peroxidase Immunodetection Kit (User Guide) (PK-2200)
M.O.M. (Mouse on Mouse) Fluorescein Immunodetection Kit (User Guide) (FMK-2201)
M.O.M. (Mouse on Mouse) Basic Immunodetection Kit (User Guide) (BMK-2202)
Protocol for Multiple Immunofluorescent Staining Using Mouse Antibodies (BMK-2202,FMK-2201)

Enzyme Substrates

ImmPACT® DAB Substrate Kit (SK-4105)
ImmPACT® DAB EqV Substrate Kit (SK-4103)
ImmPACT® AEC Substrate Kit (SK-4205)
ImmPACT® AMEC Red Substrate Kit (SK-4285)
ImmPACT® VIP Substrate Kit (SK-4605)
ImmPACT® SG Substrate Kit (SK-4705)
ImmPACT® NovaRED Substrate Kit (SK-4805)
DAB Substrate Kit (SK-4100)
DAB Enhancing Solution (H-2200-30)
Vector® VIP Substrate Kit (SK-4600)
Vector® SG Substrate Kit (SK-4700)
Vector® NovaRED Substrate Kit (SK-4800)
AEC Substrate Kit (SK-4200)
TMB Substrate Kit (User Guide) (SK-4400)
DuoLuX® Chemiluminescent/Fluorescent Substrate Kit for peroxidase (User Guide) (SK-6604-200)
ImmPACT® Vector® Red Substrate Kit (SK-5105)
Vector® Red Substrate Kit (SK-5100)
Vector® Blue Substrate Kit (SK-5300)
Vector® Black Substrate Kit (SK-5200)
BCIP/NBT Substrate Kit (SK-5400)
Levamisole Solution (SP-5000-18)
DuoLuX® Chemiluminescent/Fluorescent Substrate Kit for alkaline phosphatase (User Guide) (SK-6605-100)

Blot Detection Systems

WestVision® Peroxidase Polymer Reagents for Western Blotting (User Guide) (WB-1000-.8,WB-2000-.8)
VECTASTAIN ABC-AmP Western Blot Detection System (User Guide) (AK-6000)

In Situ Hybridization

Fluorescence Detection of Biotin-Labeled ISH Probes - Protocol (A-2011-1,BA-0300-.5)
Fluorescence Detection of Fluorescein-Labeled ISH Probes - Protocol (A-2011-1,BA-0601-.5)
Chromogenic Detection of Biotin-Labeled ISH Probes - Protocol (SA-5100-1)
In Situ Hybridization Detection Protocol (A-2011-1,BA-0300-.5,BA-0601-.5,H-1000-10,H-1200-10,H-1300-10,H-1400-10,H-1500-10,H-5000-60,H-5501-60,MB-1220-100,SA-5100-1,SK-5400,SP-5000-18,H-1700,H-1800)

Biotin Quantitation Kit

QuantTag Biotin Kit (User Guide) (BDK-2000)

Thiol-Reactive Labeling Reagents

Biotin Maleimide (User Guide) (SP-1501-12)
Fluorescein Maleimide (SP-1502-12)
Texas Red Maleimide (User Guide) (SP-1505-3.6)

Molecular Biology Reagents

3' EndTag Labeling System (User Guide) (MB-9002)

Neuronal Tracing reagents

PHA-L METHOD FOR TRACING EFFERENT NEURONAL PROJECTIONS (L-1110-5)
NEUROBIOTIN Tracer for Intracellular Labeling of Neurons (SP-1120)