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Protocols

Selected Vector kit instructions and associated protocols are listed below in PDF format. If you are unable to obtain the desired information please contact Vector technical service or your local Vector Labs distributor for further assistance.

Supplemental Protocols for Vector^® Kits and Reagents

Human on Human Immunodetection Kit (User Guide) (HOH-3000)

Antigen Unmasking Solution (Tris-based, pH 9.0) (User Guide) (H-3301-250)

Enzyme Immunoassays, Hybridoma Screening, and Western Blots

BLOXALL^® Solution for Quenching Endogenous Peroxidase and Alkaline Phosphatase Activity (SP-6000-100)

Recommended Dilutions and Buffers

Antigen Unmasking Solution (Citrate-based, pH 6.0) (User Guide) (H-3300-250)

Biotin Labeling Reagents

PHOTOPROBE ^® Biotin (User Guide) (SP-1000-.5,SP-1020-.5)

Biotin (Long Arm) NHS (SP-1200-50)

Biotin (Long Arm) NHS, water soluble (SP-1210-50)

VECTASTAIN^® Kits

VECTASTAIN Elite ABC Universal PLUS Kit (PK-8200)

VECTASTAIN Elite ABC-Peroxidase Kit (User Guide) (PK-6100,PK-6101,PK-6102,PK-6103,PK-6104,PK-6105,PK-6106)

VECTASTAIN Universal Elite ABC-Peroxidase Kit (User Guide) (PK-6200)

R.T.U. VECTASTAIN EliteABC-Peroxidase Reagent (User Guide) (PK-7100)

R.T.U. VECTASTAIN Universal Elite ABC-Peroxidase Kit (User Guide) (PK-7200)

VECTASTAIN ABC-Peroxidase Kit (User Guide) (PK-4000,PK-4001,PK-4002,PK-4003,PK-4004,PK-4005,PK-4006,PK-4007,PK-4010)

VECTASTAIN ABC-Alkaline Phosphatase Kit (User Guide) (AK-5000,AK-5001,AK-5002,AK-5003,AK-5004,AK-5005,AK-5006,AK-5007,AK-5010)

VECTASTAIN Universal ABC-Alkaline Phosphatase Kit (User Guide) (AK-5200)

VECTASTAIN Universal Quick Kit (User Guide) (PK-8800)

R.T.U. VECTASTAIN Universal Quick Kit (User Guide) (PK-7800)

Immunofluorescence

VectaFluor™ Duet Double Labeling Kit (User Guide) (DK-8818)

VectaFluor™ Duet Double Labeling Kit (User Guide) (DK-8828)

TrueVIEW^® Autofluorescence Quenching Kit (User Guide) (SP-8400-15)

TrueVIEW^® Autofluorescence Quenching Kit with DAPI (User Guide) (SP-8500-15)

Double Immunofluorescent Labeling Protocol (A-2011-1,A-2016-1)

VectaFluor™ DyLight^® 488 Anti-Rabbit Ig (DI-1788-15)

VectaFluor™ DyLight^® 594 Anti-Mouse Ig (DI-2794-15)

VectaFluor™ DyLight^® 594 Anti-Rabbit Ig (DI-1794-15)

VectaFluor™ Excel Amplified DyLight^® 488 Anti-Mouse Ig (DK-2488)

VectaFluor™ Excel Amplified DyLight^® 488 Anti-Rabbit Ig (DK-1488)

VectaFluor™ Excel Amplified DyLight^® 594 Anti-Mouse Ig (DK-2594)

VectaFluor™ Excel Amplified DyLight^® 594 Anti-Rabbit Ig (DK-1594)

VECTABOND^® Reagent for Tissue Section Adhesion (SP-1800-7)

Avidin/Biotin Blocking Kit (SP-2001)

Streptavidin/Biotin Blocking Kit (SP-2002)

Bioconjugation Reagents

Troubleshooting Guide – Bioconjugation (A-9002-001,A-9202-001,B-9007-105K,B-9007-009K,B-9014-009K,F-9001-009K,P-9002-002,S-9010-1,S-9011,S-9011-1)

Bradford Assay Protocol (A-9002-001)

General Desalting Protocol

Protein Desalting Protocol

Using a NanoDrop to Measure Antibody Concentration

Concentration of Dilute Antibody Solutions

HRP-Antibody All-in-One Conjugation Kit (User Guide) (A-9002)

R-PE Antibody Conjugation Kit (User Guide) (P-9002,P-9002-002)

BCA Protein Assay Protocol (A-9002,A-9002-001)

ImmPRESS^® Polymerized Reporter Enzyme Staining System

ImmPRESS Duet Double Staining HRP/AP Polymer Kit (User Guide) (MP-7714-15)

ImmPRESS Duet Double Staining HRP/AP Polymer Kit (User Guide) (MP-7724-15)

ImmPRESS HRP Universal PLUS Polymer Kit (User Guide) (MP-7800-15)

ImmPRESS HRP Horse Anti-Rabbit IgG PLUS Polymer Kit (User Guide) (MP-7801-15)

ImmPRESS HRP Horse Anti-Mouse IgG PLUS Polymer Kit (User Guide) (MP-7802-15)

ImmPRESS-VR HRP Horse Anti-Rabbit IgG Polymer (User Guide) (MP-6401-15)

ImmPRESS-VR HRP Horse Anti-Mouse IgG Polymer Kit (User Guide) (MP-6402-15)

ImmPRESS HRP Goat Anti-Rabbit IgG Polymer Kit (User Guide) (MP-7451)

ImmPRESS HRP Goat Anti-Mouse IgG Polymer Kit (User Guide) (MP-7452)

ImmPRESS-AP Goat Anti-Rat IgG Polymer Kit (User Guide) (MP-5404-15)

ImmPRESS-AP Horse Anti-Goat IgG Polymer Kit (User Guide) (MP-5405)

ImmPRESS-AP Goat Anti-Rat IgG (Mouse Adsorbed) Polymer Kit (User Guide) (MP-5444-15)

ImmPRESS-AP Horse Anti-Rabbit IgG Polymer Kit (User Guide) (MP-5401)

ImmPRESS-AP Horse Anti-Mouse IgG Polymer Kit (User Guide) (MP-5402)

M.O.M.^® (Mouse on Mouse) ImmPRESS Peroxidase Polymer Kit (User Guide) (MP-2400)

ImmPRESS HRP Horse Anti-Rabbit IgG Polymer Kit (User Guide) (MP-7401)

ImmPRESS HRP Horse Anti-Mouse IgG Polymer Kit (User Guide) (MP-7402)

ImmPRESS HRP Horse Anti-Mouse IgG, Rat-adsorbed Polymer Kit (User Guide) (MP-7422-15)

ImmPRESS HRP Goat Anti-Rat IgG Polymer Kit (User Guide) (MP-7404-50)

ImmPRESS HRP Goat Anti-Rat IgG, Mouse-adsorbed Polymer Kit (User Guide) (MP-7444-15)

ImmPRESS HRP Horse Anti-Goat IgG Polymer Kit (User Guide) (MP-7405)

ImmPRESS HRP Universal Polymer Kit (User Guide) (MP-7500)

ImmPRESS Excel Amplified Polymer Kit, Anti-Rabbit IgG (User Guide) (MP-7601)

ImmPRESS Excel Amplified HRP Polymer Kit, Anti-Mouse IgG (User Guide) (MP-7602)

Counterstains

Alcian Blue (pH 2.5) Stain Kit (User Guide) (H-3501)

Hematoxylin & Eosin Stain Kit (User Guide) (H-3502)

Vector^{^®} Hematoxylin (User Guide) (H-3401-500)

Vector^{^®} Hematoxylin QS (User Guide) (H-3404-100)

Vector^{^®} Methyl Green (User Guide) (H-3402-500)

Vector^{^®} Nuclear Fast Red (User Guide) (H-3403-500)

Mounting Media

VECTASHIELD^® HardSet Antifade Mounting Medium with Phalloidin (User Guide) (H-1600-10)

VECTASHIELD PLUS Antifade Mounting Medium (User Guide) (H-1900)

VECTASHIELD PLUS Antifade Mounting Medium with DAPI (User Guide) (H-2000)

VECTASHIELD^® Vibrance Antifade Mounting Medium (User Guide) (H-1700)

VECTASHIELD^® Vibrance Antifade Mounting Medium with DAPI (User Guide) (H-1800)

VectaMount^® Permanent Mounting Medium (H-5000-60)

VectaMount^® AQ Aqueous Mounting Medium (H-5501-60)

VECTASHIELD^® Mounting Media (User Guide) (H-1000-10)

VECTASHIELD^® Mounting Media with DAPI (User Guide) (H-1200-10)

VECTASHIELD^®Mounting Media with PI (User Guide) (H-1300-10)

VECTASHIELD^®HardSet Mounting Media (User Guide) (H-1400-10)

VECTASHIELD^®HardSet Mounting Media with DAPI (User Guide) (H-1500-10)

Vector Mouse on Mouse (M.O.M.^®) Immunodetection Kit

M.O.M. (Mouse on Mouse) Elite Peroxidase Immunodetection Kit (User Guide) (PK-2200)

M.O.M. (Mouse on Mouse) Fluorescein Immunodetection Kit (User Guide) (FMK-2201)

M.O.M. (Mouse on Mouse) Basic Immunodetection Kit (User Guide) (BMK-2202)

Enzyme Substrates

ImmPACT^® DAB Substrate Kit (SK-4105)

ImmPACT^® DAB EqV Substrate Kit (SK-4103)

ImmPACT^® AEC Substrate Kit (SK-4205)

ImmPACT^® AMEC Red Substrate Kit (SK-4285)

ImmPACT^® VIP Substrate Kit (SK-4605)

ImmPACT^® SG Substrate Kit (SK-4705)

ImmPACT^® NovaRED Substrate Kit (SK-4805)

DAB Substrate Kit (SK-4100)

DAB Enhancing Solution (H-2200-30)

Vector^{^®} VIP Substrate Kit (SK-4600)

Vector^{^®} SG Substrate Kit (SK-4700)

Vector^{^®} NovaRED Substrate Kit (SK-4800)

AEC Substrate Kit (SK-4200)

TMB Substrate Kit (User Guide) (SK-4400)

DuoLuX^® Chemiluminescent/Fluorescent Substrate Kit for peroxidase (User Guide) (SK-6604-200)

ImmPACT^® Vector^{^®} Red Substrate Kit (SK-5105)

Vector^{^®} Red Substrate Kit (SK-5100)

Vector^{^®} Blue Substrate Kit (SK-5300)

Vector^{^®} Black Substrate Kit (SK-5200)

BCIP/NBT Substrate Kit (SK-5400)

Levamisole Solution (SP-5000-18)

DuoLuX^® Chemiluminescent/Fluorescent Substrate Kit for alkaline phosphatase (User Guide) (SK-6605-100)

Blot Detection Systems

WestVision^® Peroxidase Polymer Reagents for Western Blotting (User Guide) (WB-1000-.8,WB-2000-.8)

VECTASTAIN ABC-AmP Western Blot Detection System (User Guide) (AK-6000)

In Situ Hybridization

Fluorescence Detection of Biotin-Labeled ISH Probes - Protocol (A-2011-1,BA-0300-.5)

Fluorescence Detection of Fluorescein-Labeled ISH Probes - Protocol (A-2011-1,BA-0601-.5)

Chromogenic Detection of Biotin-Labeled ISH Probes - Protocol (SA-5100-1)

In Situ Hybridization Detection Protocol (A-2011-1,BA-0300-.5,BA-0601-.5,H-1000-10,H-1200-10,H-1300-10,H-1400-10,H-1500-10,H-5000-60,H-5501-60,MB-1220-100,SA-5100-1,SK-5400,SP-5000-18,H-1700,H-1800)

Biotin Quantitation Kit

QuantTag Biotin Kit (User Guide) (BDK-2000)

Thiol-Reactive Labeling Reagents

Biotin Maleimide (User Guide) (SP-1501-12)

Fluorescein Maleimide (User Guide) (SP-1502-12)

Texas Red Maleimide (User Guide) (SP-1505-3.6)

Molecular Biology Reagents

5' EndTag Labeling System (User Guide) (MB-9001)

3' EndTag Labeling System (User Guide) (MB-9002)

Neuronal Tracing reagents

PHA-L METHOD FOR TRACING EFFERENT NEURONAL PROJECTIONS (L-1110-5)

NEUROBIOTIN Tracer for Intracellular Labeling of Neurons (SP-1120)

Lectins

Peroxidase localization of WGA in Neural Tissue (L-1020)

Agarose WGA - Use and Regeneration (AL-1023)

Enrichment of NK cells by panning on ECL plates (L-1140-10)

Specificity of Jacalin binding (L-1150)