Protocols
Selected Vector kit instructions and associated protocols are listed below in PDF format. If you are unable to obtain the desired information please contact Vector technical service or your local Vector Labs distributor for further assistance.
Supplemental Protocols for Vector® Kits and Reagents
Biotin Labeling Reagents
PHOTOPROBE ® Biotin (User Guide)
(SP-1000-.5,SP-1020-.5)
Biotin (Long Arm) NHS
(SP-1200-50)
Biotin (Long Arm) NHS, water soluble
(SP-1210-50)
VECTASTAIN® Kits
VECTASTAIN Elite ABC Universal PLUS Kit
(PK-8200)
VECTASTAIN Elite ABC-Peroxidase Kit (User Guide)
(PK-6100,PK-6101,PK-6102,PK-6103,PK-6104,PK-6105,PK-6106)
VECTASTAIN ABC-Peroxidase Kit (User Guide)
(PK-4000,PK-4001,PK-4002,PK-4003,PK-4004,PK-4005,PK-4006,PK-4007,PK-4010)
VECTASTAIN ABC-Alkaline Phosphatase Kit (User Guide)
(AK-5000,AK-5001,AK-5002,AK-5003,AK-5004,AK-5005,AK-5006,AK-5007,AK-5010)
Immunofluorescence
Double Immunofluorescent Labeling Protocol
(A-2011-1,A-2016-1)
VectaFluor™ DyLight® 488 Anti-Rabbit Ig
(DI-1788-15)
VectaFluor™ DyLight® 594 Anti-Mouse Ig
(DI-2794-15)
VectaFluor™ DyLight® 594 Anti-Rabbit Ig
(DI-1794-15)
VECTABOND® Reagent for Tissue Section Adhesion
(SP-1800-7)
Avidin/Biotin Blocking Kit
(SP-2001)
Streptavidin/Biotin Blocking Kit
(SP-2002)
Bioconjugation Reagents
R-PE Antibody Conjugation Kit (User Guide)
(P-9002,P-9002-002)
ImmPRESS® Polymerized Reporter Enzyme Staining System
ImmPRESS HRP Universal PLUS Polymer Kit (User Guide)
(MP-7800-15)
Counterstains
Vector® Hematoxylin (User Guide)
(H-3401-500)
Vector® Hematoxylin QS (User Guide)
(H-3404-100)
Vector® Methyl Green (User Guide)
(H-3402-500)
Vector® Nuclear Fast Red (User Guide)
(H-3403-500)
Mounting Media
VectaMount® Permanent Mounting Medium
(H-5000-60)
VectaMount® AQ Aqueous Mounting Medium
(H-5501-60)
VECTASHIELD® Mounting Media (User Guide)
(H-1000-10)
VECTASHIELD®Mounting Media with PI (User Guide)
(H-1300-10)
VECTASHIELD®HardSet Mounting Media (User Guide)
(H-1400-10)
Vector Mouse on Mouse (M.O.M.®) Immunodetection Kit
Enzyme Substrates
ImmPACT® DAB Substrate Kit
(SK-4105)
ImmPACT® DAB EqV Substrate Kit
(SK-4103)
ImmPACT® AEC Substrate Kit
(SK-4205)
ImmPACT® AMEC Red Substrate Kit
(SK-4285)
ImmPACT® VIP Substrate Kit
(SK-4605)
ImmPACT® SG Substrate Kit
(SK-4705)
ImmPACT® NovaRED Substrate Kit
(SK-4805)
DAB Substrate Kit
(SK-4100)
DAB Enhancing Solution
(H-2200-30)
Vector® VIP Substrate Kit
(SK-4600)
Vector® SG Substrate Kit
(SK-4700)
Vector® NovaRED Substrate Kit
(SK-4800)
AEC Substrate Kit
(SK-4200)
TMB Substrate Kit (User Guide)
(SK-4400)
ImmPACT® Vector® Red Substrate Kit
(SK-5105)
Vector® Red Substrate Kit
(SK-5100)
Vector® Blue Substrate Kit
(SK-5300)
Vector® Black Substrate Kit
(SK-5200)
BCIP/NBT Substrate Kit
(SK-5400)
Levamisole Solution
(SP-5000-18)
Blot Detection Systems
WestVision® Peroxidase Polymer Reagents for Western Blotting (User Guide)
(WB-1000-.8,WB-2000-.8)
In Situ Hybridization
Fluorescence Detection of Biotin-Labeled ISH Probes - Protocol
(A-2011-1,BA-0300-.5)
Fluorescence Detection of Fluorescein-Labeled ISH Probes - Protocol
(A-2011-1,BA-0601-.5)
In Situ Hybridization Detection Protocol
(A-2011-1,BA-0300-.5,BA-0601-.5,H-1000-10,H-1200-10,H-1300-10,H-1400-10,H-1500-10,H-5000-60,H-5501-60,MB-1220-100,SA-5100-1,SK-5400,SP-5000-18,H-1700,H-1800)
Biotin Quantitation Kit
QuantTag Biotin Kit (User Guide)
(BDK-2000)
Thiol-Reactive Labeling Reagents
Biotin Maleimide (User Guide)
(SP-1501-12)
Fluorescein Maleimide (User Guide)
(SP-1502-12)
Texas Red Maleimide (User Guide)
(SP-1505-3.6)
Molecular Biology Reagents
5' EndTag Labeling System (User Guide)
(MB-9001)
3' EndTag Labeling System (User Guide)
(MB-9002)
Neuronal Tracing reagents
Lectins
Agarose WGA - Use and Regeneration
(AL-1023)
Enrichment of NK cells by panning on ECL plates
(L-1140-10)
Specificity of Jacalin binding
(L-1150)