In Situ Hybridization Workflow
Due to their high affinity, high sensitivity, and low background, fluorescence- and enzyme-based detection reagents from Vector Laboratories are ideal for in situ hybridization (ISH) applications.
Confirm probe customization and label incorporation. For example, use the QuantTag Biotin Quantitation kit to determine the number of biotin molecules incorporated in each strand of nucleic acid.
Reduce or eliminate unwanted background (non-specific) staining on tissue sections and cell preparations using a blocking solution. Non-specific staining can result from endogenous enzyme or tissue elements, including endogenous enzyme activity, presence of Fc receptors, or interactions of detection reagents with tissue or cell proteins and other macromolecules. Choose a blocking solution based on the results of negative control sections.Blocking Reagents
Use fluorescent or enzyme-conjugated reagents that meet your sensitivity requirements and visualization preferences.Fluorophore Conjugated Avidin/Streptavidin Enzyme Conjugated Avidin/Streptavidin Anti-Avidin/Streptavidin Reagents Antibodies to Tags & Labels Secondary Antibodies VECTASTAIN® ABC Kits (HRP) VECTASTAIN® ABC AP Kits (AP) ImmPRESS® Polymer Reagents for IHC