ImmPRESS® Polymer Reagents for IHC

Integrated antibody and enzyme technology for high-sensitivity IHC detection

Polymer-based reagents are a more recent introduction into IHC detection methodology than traditional avidin and biotin conjugates, such as ABC kit formats. Polymers offer distinct advantages over these traditional methods particularly for applications such as multiple antigen labeling (multiplexing) on the same tissue section, or in instances where detectable levels of endogenous biotin may be problematic.

Polymer-based systems essentially consist of an integrated polymer of active enzyme and secondary antibody that binds to a primary antibody target. This integrated format introduces significantly more enzyme at the site of localization, thereby generating a greater reaction with the subsequent chromogen, as compared to the use of a secondary antibody directly conjugated with enzyme. Additionally, use of a one-step polymer method shortens the IHC procedure by avoiding the two-step biotinylated secondary antibody and ABC reagent step that is required for standard avidin-biotin systems.

ImmPRESS Polymer Detection Kits require little optimization and facilitate quick, efficient detection in cell- and tissue-based applications.

These kits offer

  • High sensitivity for optimum immunohistochemistry detection
  • Unique, non-biotin based micropolymer technology
  • Ready-to-use, pre-diluted reagents
  • Time-saving one-step protocols
  • Choice of enzyme (HRP or AP)

Which kit is right for you?

One-Step Detection

Species-specific polymer detection kits. These kits enable selection for:

  • Single antigen staining
  • Sequential double staining
  • Reducing/eliminating cross-reactivity issues

Learn more:
ImmPRESS Alkaline Phosphatase (AP) Polymer Reagents

ImmPRESS Horseradish Peroxidase (HRP) Polymer Reagents

Two-Step Amplified Detection

Horseradish Peroxidase (HRP) polymer reagent plus anti-mouse or anti-rabbit amplifier antibody for increased sensitivity. These kits enable selection for:

  • Unknown or low antigen expression
  • Maximizing primary antibody dilution
  • Convenience of complete kit format

Learn more:
ImmPRESS Excel Amplified Polymer Staining System

Double Staining

HRP- and AP- anti-mouse and anti-rabbit polymer reagent cocktail for staining two antigens in a single step. These kits enable selection for:

  • Double antigen staining
  • Streamlining and shortening a double labeling protocol

Learn more:
ImmPRESS Duet Double Staining Polymer Kits

Species-on-Species Detection

Mouse Ig blocking and anti-mouse IgG HRP polymer reagent for visualization of mouse primary antibodies on mouse tissue sections. Use these kits to select for:

  • Transgene and knock-out mice models
  • Genetically modified mice
  • Mouse xenograft tissue
  • Normal mouse tissue

Learn more:
M.O.M.® (Mouse on Mouse) ImmPRESS Polymer Reagent, Anti-Mouse IgG, Peroxidase, Catalog Number MPX-2402-15

M.O.M.® (Mouse on Mouse) ImmPRESS HRP (Peroxidase) Polymer Kit, Catalog Number MP-2400

One-Step Detection Kits
Species-specific, enzyme conjugated secondary antibody polymers.
Two-Step Amplified Detection Kits
Anti-mouse and anti-rabbit IgG HRP conjugated secondary antibody polymers with additional amplifying reagent.
Double Staining Kits
Anti-mouse and anti-rabbit IgG HRP and AP conjugated secondary polymer cocktail.

Explore ImmPRESS Polymer Detection Kits for IHC

Choose from our selection on species-specific and 15 or 50 mL volume formats of ImmPRESS AP polymer kits.

Choose from our wide selection of species-specific, universal and highly cross-adsorbed (VR series) ImmPRESS HRP polymer kits.

ImmPRESS™ Excel Amplified HRP (peroxidase) Polymer Staining Kits are complete kits that include key detection reagents including ImmPACT DAB and ImmPACT Vector Red substrates.

ImmPRESS™ Duet Double Staining Polymer Kits are offered as complete kits that include ImmPACT DAB and ImmPACT Vector Red substrates.


Resources

Immunohistochemistry Resource Guide

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
Learn more about this guide >

IHC Troubleshooting Guide for Polymer Kits

A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol polymer detection systems.  Also troubleshoot issues of weak or no staining. Laminated.

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ImmPRESS® Polymer Detection Brochure

This ImmPRESS Enzyme Polymer brochure describes ImmPRESS micropolymer technology and includes schematics to help the user choose the most appropriate format for their application.

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