ImmPRESS® Polymer Reagents for IHC
Integrated antibody and enzyme technology for high-sensitivity IHC detection
Polymer-based reagents are a more recent introduction into IHC detection methodology than traditional avidin and biotin conjugates, such as ABC kit formats. Polymers offer distinct advantages over these traditional methods particularly for applications such as multiple antigen labeling (multiplexing) on the same tissue section, or in instances where detectable levels of endogenous biotin may be problematic.
Polymer-based systems essentially consist of an integrated polymer of active enzyme and secondary antibody that binds to a primary antibody target. This integrated format introduces significantly more enzyme at the site of localization, thereby generating a greater reaction with the subsequent chromogen, as compared to the use of a secondary antibody directly conjugated with enzyme. Additionally, use of a one-step polymer method shortens the IHC procedure by avoiding the two-step biotinylated secondary antibody and ABC reagent step that is required for standard avidin-biotin systems.
ImmPRESS Polymer Detection Kits require little optimization and facilitate quick, efficient detection in cell- and tissue-based applications.
These kits offer
- High sensitivity for optimum immunohistochemistry detection
- Unique, non-biotin based micropolymer technology
- Ready-to-use, pre-diluted reagents
- Time-saving one-step protocols
- Choice of enzyme (HRP or AP)
Which kit is right for you?
Species-specific polymer detection kits. These kits enable selection for:
- Single antigen staining
- Sequential double staining
- Reducing/eliminating cross-reactivity issues
Horseradish Peroxidase (HRP) polymer reagent plus anti-mouse or anti-rabbit amplifier antibody for increased sensitivity. These kits enable selection for:
- Unknown or low antigen expression
- Maximizing primary antibody dilution
- Convenience of complete kit format
HRP- and AP- anti-mouse and anti-rabbit polymer reagent cocktail for staining two antigens in a single step. These kits enable selection for:
- Double antigen staining
- Streamlining and shortening a double labeling protocol
Mouse Ig blocking and anti-mouse IgG HRP polymer reagent for visualization of mouse primary antibodies on mouse tissue sections. Use these kits to select for:
- Transgene and knock-out mice models
- Genetically modified mice
- Mouse xenograft tissue
- Normal mouse tissue
One-Step Detection Kits
Species-specific, enzyme conjugated secondary antibody polymers.
Two-Step Amplified Detection Kits
Anti-mouse and anti-rabbit IgG HRP conjugated secondary antibody polymers with additional amplifying reagent.
Double Staining Kits
Anti-mouse and anti-rabbit IgG HRP and AP conjugated secondary polymer cocktail.
Resources
This ImmPRESS Enzyme Polymer brochure describes ImmPRESS micropolymer technology and includes schematics to help the user choose the most appropriate format for their application.
