Integrated antibody and enzyme technology for high-sensitivity IHC detection
Polymer-based reagents are a more recent introduction into IHC detection methodology than traditional avidin and biotin conjugates, such as ABC kit formats. Polymers offer distinct advantages over these traditional methods particularly for applications such as multiple antigen labeling (multiplexing) on the same tissue section, or in instances where detectable levels of endogenous biotin may be problematic.
Polymer-based systems essentially consist of an integrated polymer of active enzyme and secondary antibody that binds to a primary antibody target. This integrated format introduces significantly more enzyme at the site of localization, thereby generating a greater reaction with the subsequent chromogen, as compared to the use of a secondary antibody directly conjugated with enzyme. Additionally, use of a one-step polymer method shortens the IHC procedure by avoiding the two-step biotinylated secondary antibody and ABC reagent step that is required for standard avidin-biotin systems.
ImmPRESS Polymer Detection Kits require little optimization and facilitate quick, efficient detection in cell- and tissue-based applications.
These kits offer
High sensitivity for optimum immunohistochemistry detection
Unique, non-biotin based micropolymer technology
Ready-to-use, pre-diluted reagents
Time-saving one-step protocols
Choice of enzyme (HRP or AP)
Which kit is right for you?
One-Step Detection
Species-specific polymer detection kits. These kits enable selection for:
Horseradish Peroxidase (HRP) polymer reagent plus anti-mouse or anti-rabbit amplifier antibody for increased sensitivity. These kits enable selection for:
Mouse Ig blocking and anti-mouse IgG HRP polymer reagent for visualization of mouse primary antibodies on mouse tissue sections. Use these kits to select for:
Transgene and knock-out mice models
Genetically modified mice
Mouse xenograft tissue
Normal mouse tissue
Learn more: M.O.M.® (Mouse on Mouse) ImmPRESS Polymer Reagent, Anti-Mouse IgG, Peroxidase, Catalog Number MPX-2402-15
ImmPRESS™ Excel Amplified HRP (peroxidase) Polymer Staining Kits are complete kits that include key detection reagents including ImmPACT DAB and ImmPACT Vector Red substrates.
This ImmPRESS Enzyme Polymer brochure describes ImmPRESS micropolymer technology and includes schematics to help the user choose the most appropriate format for their application.