ImmPRESS® Polymer Reagents for IHC

Integrated antibody and enzyme technology for high-sensitivity IHC detection

Polymer-based reagents are a more recent introduction into IHC detection methodology than traditional avidin and biotin conjugates, such as ABC kit formats. Polymers offer distinct advantages over these traditional methods particularly for applications such as multiple antigen labeling (multiplexing) on the same tissue section, or in instances where detectable levels of endogenous biotin may be problematic.

Polymer-based systems essentially consist of an integrated polymer of active enzyme and secondary antibody that binds to a primary antibody target. This integrated format introduces significantly more enzyme at the site of localization, thereby generating a greater reaction with the subsequent chromogen, as compared to the use of a secondary antibody directly conjugated with enzyme. Additionally, use of a one-step polymer method shortens the IHC procedure by avoiding the two-step biotinylated secondary antibody and ABC reagent step that is required for standard avidin-biotin systems.

ImmPRESS Polymer Detection Kits require little optimization and facilitate quick, efficient detection in cell- and tissue-based applications.

These kits offer

• High sensitivity for optimum immunohistochemistry detection
• Unique, non-biotin based micropolymer technology
• Ready-to-use, pre-diluted reagents
• Time-saving one-step protocols
• Choice of enzyme (HRP or AP)