Anti-Avidin/Streptavidin Reagents

Amplify avidin and streptavidin signals in a range of applications

Use Anti-Avidin or Anti-Streptavidin amplification reagents to increase sensitivity in streptavidin or avidin/biotin detection systems. Anti-avidin does not bind streptavidin, and anti-streptavidin does not recognize avidin. Avidin or streptavidin amplification reagents can amplify signals in many applications, including:

  • Immunohistochemistry 
  • In situ hybridization
  • Microarray assays
  • ELISAs
  • Blots

Vector offers anti-avidin and anti-streptavidin antibodies in biotinylated, fluorophore-conjugated, or unconjugated formats. The biotinylated formats are unique in that they allow binding to streptavidin or avidin through both of their antigen-binding sites and the covalently-attached biotin residues.

FastTag Biotin-conjugated human chromosome 1 centromere-specific probe detected with Texas Red Avidin DCS, Biotinylated Anti-Avidin and Texas Red Avidin DCS (red). Mounted in VECTASHIELD Mounting Medium with DAPI (blue).

Resources

Immunofluorescence Resource Guide

Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents.  This guide will help you:

  • Learn about the IF workflow and how to optimize each step
  • Quickly identify and choose the most appropriate fluorescent secondary conjugates
  • Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving

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TrueVIEW Autofluorescence Quencher Brochure

For those wanting additional details on the simple protocol and mode of action of the TrueVIEW Autofluorescence Quenching Kit, this brochure contains clear, concise scematics, comparision data with other types of quenchers, and customer testimonials.

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