Protein Blocking Solutions
Non-specific protein interactions between detection reagents and target protein elements can cause unwanted background staining. To minimize this type of interaction, use an appropriate protein blocking reagent:
- Normal sera — widely used as a general protein block in tissue and cell-based staining. For best results, choose a serum species for blocking that matches the species of the secondary antibody.
- Carbo-Free Blocking Solution (SP-5040) — for lectin staining or in multiplex IHC applications. Lectins are used for at least one label because this solution does not contain interfering glycoproteins.
- BSA (SP-5050) — free of the glycoproteins and impurities present in other grades of BSA that can introduce artifacts or increase background staining. Use in IHC, iCC, IF, blots, and ELISAs.
- R.T.U. Animal-Free Block and Diluent (SP-5035) — plant-protein derived universal antibody diluent and protein blocking reagent for use in multiplex IHC applications where primaries or secondaries from multiple species are used. This solution does not contain animal immunoglobulins, which may be non-specifically bound by the secondary antibodies.
Resources
Immunohistochemistry Resource Guide
The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
Troubleshooting Guide for ABC Systems
A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol using ABC systems. Also troubleshoot issues of weak or no staining. Laminated.
Troubleshooting Guide for Polymer Kits
A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol polymer detection systems. Also troubleshoot issues of weak or no staining. Laminated.