ImmPRESS® Polymer Reagents for IHC
Integrated antibody and enzyme technology for high-sensitivity IHC detection
Polymer-based reagents offer distinct advantages over traditional methods. This is particularly true for applications such as multiple antigen labeling (multiplexing) or where detectable levels of endogenous biotin may be problematic.
Polymer-based systems consist of an integrated polymer of active enzyme and secondary antibody that binds to a primary antibody target. This integrated format introduces significantly more enzyme at the site of localization, thereby generating a greater reaction with the subsequent chromogen, as compared to the use of a secondary antibody directly conjugated with enzyme.
Additionally, use of a one-step polymer method shortens the IHC staining procedure by avoiding the two-step biotinylated secondary antibody and ABC reagent step that is required for standard avidin-biotin systems.
ImmPRESS Polymer Detection Kits require little optimization and facilitate quick, efficient detection in cell- and tissue-based applications.
These kits offer
- High sensitivity for optimum immunohistochemistry detection
- Unique, non-biotin based micropolymer technology
- Ready-to-use, pre-diluted reagents
- Time-saving one-step protocols
- Choice of enzyme (HRP or AP)
Which kit is right for you?
Species-specific polymer detection kits.
These kits enable selection for:
- Single antigen staining
- Sequential double staining
- Reducing/eliminating cross-reactivity issues
Discover ImmPRESS AP Polymer Reagents:
Horseradish Peroxidase (HRP) polymer reagent plus anti-mouse or anti-rabbit amplifier antibody for increased sensitivity.
These kits enable selection for:
- Unknown or low antigen expression
- Maximizing primary antibody dilution
- Convenience of complete kit format
Discover ImmPRESS Excel Polymer Reagents:
HRP- and AP- anti-mouse and anti-rabbit polymer reagent cocktail for staining two antigens in a single step.
These kits enable selection for:
- Double antigen staining
- Streamlining and shortening a double labeling protocol
Discover ImmPRESS Duet Double Staining Reagent Kits:
Mouse Ig blocking and anti-mouse IgG HRP polymer reagent for visualization of mouse primary antibodies on mouse tissue sections.
Use these kits to select for:
- Transgene and knock-out mice models
- Genetically modified mice
- Mouse xenograft tissue
- Normal mouse tissue
Discover M.O.M.® ImmPRESS Polymer Reagent Kits:
M.O.M.® (Mouse on Mouse) ImmPRESS Polymer Reagent, Anti-Mouse IgG, Peroxidase
M.O.M.® (Mouse on Mouse) ImmPRESS HRP (Peroxidase) Polymer Kit
One-Step Detection Kits
Two-Step Amplified Detection Kits
Double Staining Kits
Explore ImmPRESS Polymer Detection Kits for IHC
Choose from our selection of species-specific and 15 or 50 mL volume formats of ImmPRESS AP polymer kits.
ImmPRESS Excel Amplified HRP (peroxidase) Polymer Staining Kits are complete kits that include key detection reagents including ImmPACT DAB and ImmPACT Vector Red substrates.
Choose from our wide selection of species-specific, universal, and highly cross-adsorbed (VR series) ImmPRESS HRP polymer kits.
ImmPRESS Duet Double Staining Polymer Kits are offered as complete kits that include ImmPACT DAB and ImmPACT Vector Red substrates.
Resources
One-step, two-step, amplify: How to choose the right IHC detection system
Enhancing the signal-to-noise ratio is essential for obtaining viable data. Choosing the appropriate immunohistochemistry detection system can help.
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Top Considerations for Selecting Immunohistochemistry (IHC) Detection Reagents
Choosing the appropriate immunohistochemistry detection reagent can be a daunting task. Here are some considerations to guide you on your path to staining success.
Read More
A Simple Guide to Immunohistochemistry Substrate Selection
This blog discusses different factors to consider—enzyme, sensitivity, color, method of visualization, and stability—when selecting the appropriate substrate (and, consequently, color) for your experiment.
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The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
This ImmPRESS Enzyme Polymer brochure describes ImmPRESS micropolymer technology and includes schematics to help the user choose the most appropriate format for their application.
