SoluLink Bioconjugation Reagents

SoluLINK^® bioconjugation technology has been engineered to allow rapid and quantifiable conjugation of all classes of biomolecules. This technology is based on the reaction of HyNic with 4FB to form a stable bis-arylhydrazone bond. Unlike other conjugation chemistries, these linkers are chromophoric, meaning that they absorb light and can therefore be easily quantified with a simple spectrophotometer reading. The bis-arylhydrazone chromophore is crucial for measuring the number of linkers incorporated on each biomolecule and the degree of conjugation between two biomolecules. Quantifiable linkers allow unparalleled reproducibility of bioconjugate preparation from batch-to-batch.

Purification to remove unincorporated labels is crucial to this process as it results in reduced non-specific binding, higher sensitivity, and higher signal-to-noise in your assay. Despite their apparent simplicity, competing “mix and use” conjugation products simply do not work well because of inefficient conjugation chemistry and the lack of a purification step. This leaves both unconjugated biomolecule (e.g., antibody) and excess label (e.g., fluor, oligo, and enzyme) in the reaction mixture.

ChromaLINK^® Biotin and Digoxigenin are the only reagents of their type that can be directly quantified upon linking to biomolecules. These reagents contain the same bis-arylhydrazone bond that is central to SoluLINK bioconjugation technology. Biotinylated biomolecules are efficiently immobilized onto streptavidin coated surfaces, such as magnetic beads and agarose.

NanoLINK^® and MagnaLINK^® streptavidin magnetic beads possess up to 15-times greater biotin binding capacity than other commercially available products. Streptavidin Agarose Ultra Performance™ also has the highest biotin binding capacity of any agarose bead on the market.

SoluLINK conjugation kits contain everything needed to produce and purify high quality conjugates. Conjugation linkers and accessories are also available as flexible stand-alone products for scale-up work. These linkers are ideal for protein crosslinking, protein labeling, chemical crosslinking, protein crosslinking, and antibody conjugation. All SoluLINK products are manufactured under the TriLink ISO 9001:2015 quality system to ensure a consistent supply of high-quality product.

Streptavidin Magnetic Beads & Affinity Resins

Product	Image	Details
MagnaLINK® Streptavidin Magnetic Beads (2.8 µm) SKU: M-1003-010 MagnaLINK 2.8 µm Streptavidin Magnetic Beads offer the highest biotin binding capacity on the market, combined with exceptional size uniformity.		View product
NanoLINK® Streptavidin Magnetic Beads (1.0 µm) SKUs: M-1002-010, M-1002-020, M-1002-050, M-1002-100 NanoLINK 1.0 µm Streptavidin Magnetic Beads have the highest biotin binding capacity on the market.		View product
Streptavidin Agarose Ultra Performance SKU: N-1000-002 Streptavidin Agarose Ultra Performance provides high biotin binding capacity at a low price.		View product

SoluLINK® Cross-Linkers & Buffers

Product	Image	Details
S-4FB Linker (DMF Soluble) SKUs: S-1004-105, S-1004-010 The S-4FB heterobifunctional crosslinker is fundamental to the SoluLINK bioconjugation technology.		View product
S-HyNic Linker (DMF Soluble) SKUs: S-1002-105, S-1002-010 The S-HyNic heterobifunctional crosslinker is fundamental to the SoluLINK bioconjugation technology.		View product
Sulfo S-4FB Linker (Water Soluble) SKUs: S-1008-105, S-1008-010 The S-4FB heterobifunctional crosslinker is fundamental to the SoluLINK bioconjugation technology.		View product
Sulfo S-HyNic Linker (Water Soluble) SKU: S-1011-010 The S-HyNic heterobifunctional crosslinker is fundamental to the SoluLINK bioconjugation technology.		View product
MHPH (Maleimide HyNic) Linker SKU: S-1009-010 MHPH (Maleimide HyNic) converts thiol groups on proteins and surfaces to HyNic groups, which is one of the fundamental linkers to SoluLINK bioconjugation technology.		View product
Conjugation Buffer (10X) SKU: S-4002-005 Conjugation Buffer is a high buffering capacity solution that is optimized for the conjugation reaction between HyNic and 4FB groups.		View product
Modification Buffer (10X) SKU: S-4000-005 Modification Buffer is an amine-free, high buffering capacity solution which is optimized for the reaction of NHS esters and TFP esters with amino groups.		View product
Anhydrous DMF SKU: S-4001-005 Anhydrous DMF (Dimethylformamide) is used for solubilizing S-HyNic, S-4FB, and other SoluLINK linkers.		View product
TurboLINK Catalyst Buffer (10X) SKU: S-2006-105 TurboLINK Catalyst Buffer is used to catalyze the SoluLINK® bioconjugation reactions, which are based on aromatic hydrazone formation between HyNic-modified biomolecules and 4FB-modified biomolecules.		View product

ChromaLINK® Reagents & Conjugation Kits

Product	Image	Details
ChromaLINK® Biotin (DMF Soluble) SKUs: B-1001-105, B-1001-010 ChromaLINK Biotin contains a UV-traceable chromophore based on ChromaLINK technology, which enables reproducibility in your biotinylation process.		View product
Sulfo ChromaLINK® Biotin (Water Soluble) SKU: B-1007-105 ChromaLINK Biotin contains a UV-traceable chromophore based on ChromaLINK technology to enable reproducibility in your biotinylation process.		View product
ChromaLINK® Biotin Maleimide SKU: B-1012-010 ChromaLINK Biotin contains a UV-traceable chromophore based on ChromaLINK technology to enable reproducibility in your biotinylation process.		View product
ChromaLINK® Biotin Protein Labeling Kit SKU: B-9007-105K The ChromaLINK Biotin Protein Labeling Kit includes everything you need to biotinylate your protein: buffers, reagents, spin columns, and an easy-to-follow protocol and online calculator.		View product
ChromaLINK® One-Shot™ Antibody Biotinylation Kit SKU: B-9007-009K The ChromaLINK One-Shot Antibody Biotinylation Kit provides convenient, consistent, and measurable biotinylation of 100 µg of antibody.		View product
ChromaLINK® Digoxigenin One-Shot™ Antibody Labeling Kit SKU: B-9014-009K The ChromaLINK Digoxigenin One-Shot Antibody Labeling Kit provides convenient, consistent, and measurable digoxigenin labeling of 100 µg of antibody.		View product
Antibody-Oligonucleotide All-in-One™ Conjugation Kit SKU: A-9202-001 The Antibody-Oligonucleotide All-in-One Conjugation Kit uses patented SoluLINK® bioconjugation technology to link an antibody to an oligonucleotide.		View product
Protein-Oligonucleotide Conjugation Kit SKU: S-9011-1 The Protein-Oligo Conjugation Kit is designed to easily and efficiently prepare two separate protein-oligo conjugates.		View product