VECTASTAIN® ABC AP Kits (AP)

High-sensitivity avidin-biotin kits with alkaline phosphatase-based detection

VECTASTAIN® ABC-AP (alkaline phosphatase) Kits provide high sensitivity avidin-biotin based detection in immunohistochemistry (IHC), immunocytochemistry (ICC), protein and nucleic acid blot, in situ hybridization (ISH), and enzyme immunoassay (ELISA) detection.

  • Alkaline phosphatase enzyme detection system
  • Avidin/biotin technology
  • High sensitivity/low background
  • Low cost
  • Can be used as a second label in multiple antigen labeling applications

Use the VECTASTAIN® ABC-AP Kits for tissues with high endogenous peroxidase activity. The system also offers additional substrate color choices.

  • To detect a biotinylated target, use the VECTASTAIN® ABC Standard Kit, plus an appropriate substrate
  • To detect an unlabeled primary antibody or lectin, use a biotinylated secondary antibody (binds the primary antibody species or lectin you have chosen), VECTASTAIN® ABC reagent, and appropriate substrate (for example, to detect a rabbit primary antibody, choose the VECTASTAIN® ABC Kit, Rabbit IgG).
Lymph Node: • Ki67 (m), VECTASTAIN® Universal ABC-AP Kit, Vector® Red Substrate (red) • Multi-cytokeratin (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Tumor: • Ki67 (m), VECTASTAIN® Elite® ABC Kit, Vector® DAB (brown) • CD34 (m), VECTASTAIN® ABC-AP Kit, Vector® Blue (blue) • Cytokeratin AE1/AE3 (m), VECTASTAIN® ABC-AP Kit, Vector® Red (red).

Resources

Immunohistochemistry Resource Guide

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.

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IHC Multiplexing Guide

A valuable resource for those considering localizing two or more antigens in the same tissue section. Protocols for chromogenic detection are accompanied by schematic illustrations of the procedures. A chart of all pair-wise combinations of substrates is included along with photographic examples. Practical tips regarding order of substrates, blocking, and proper controls are also provided.

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Troubleshooting Guide for ABC Systems

A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol using ABC systems.  Also troubleshoot issues of weak or no staining. Laminated.

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