High-sensitivity avidin-biotin kits with alkaline phosphatase-based detection

VECTASTAIN® ABC-AP (alkaline phosphatase) Kits provide high sensitivity avidin-biotin based detection in immunohistochemistry (IHC), immunocytochemistry (ICC), protein and nucleic acid blot, in situ hybridization (ISH), and enzyme immunoassay (ELISA) detection. In addition to their inclusion of the Alkaline Phosphatase enzyme detection system and use of avidin / biotin technology, these low-cost kits are also highly sensitive with low background signal. These kits can also be used as a second label in multiple antigen labeling applications.

Application Note

Use the VECTASTAIN® ABC-AP Kits for tissues with high endogenous peroxidase activity. This system also offers additional substrate color choices.

  • To detect a biotinylated target, use the VECTASTAIN® ABC Standard Kit along with an appropriate substrate
  • To detect an unlabeled primary antibody or lectin, use a biotinylated secondary antibody (binds the primary antibody species or lectin you have chosen), VECTASTAIN® ABC reagent, and an appropriate substrate (for example, to detect a rabbit primary antibody, choose the VECTASTAIN® ABC Kit, Rabbit IgG).
Lymph Node: • Ki67 (m), VECTASTAIN® Universal ABC-AP Kit, Vector® Red Substrate (red) • Multi-cytokeratin (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Tumor: • Ki67 (m), VECTASTAIN® Elite® ABC Kit, Vector® DAB (brown) • CD34 (m), VECTASTAIN® ABC-AP Kit, Vector® Blue (blue) • Cytokeratin AE1/AE3 (m), VECTASTAIN® ABC-AP Kit, Vector® Red (red).


IHC Multiplexing Guide

A valuable resource for those considering localizing two or more antigens in the same tissue section. Protocols for chromogenic detection are accompanied by schematic illustrations of the procedures.
A chart of all pair-wise combinations of substrates is included along with photographic examples. Practical tips regarding order of substrates, blocking, and proper controls are also provided.

IHC Troubleshooting Guide for ABC Systems

A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol using ABC systems.  Also troubleshoot issues of weak or no staining. Laminated.