Mounting Media

Selection of mounting media for immunohistochemistry and immunofluorescence applications

The right mounting medium protects your signal and your samples while viewing and for short- and long-term archiving. Vector Laboratories offers you a selection of mounting media, each optimized for either immunohistochemistry (IHC) or immunofluorescence (IF).

Colon: CD3 (rm), ImmPRESS Reagent (HRP) Anti-Rabbit IgG, ImmPACT SG (blue-gray); and Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP) Anti-Mouse IgG, ImmPACT AMEC Red Substrate (red).
Human embryonic stem cell (hESC)-derived neurons stained with Brn3a (Green), Peripherin (Red), Beta-3 Tubulin (Magenta), and DAPI (blue). Image captured by Michael Yee, Kinchington Lab, Department of Ophthalmology, University of Pittsburgh, USA.

Explore Mounting Media

When applying a coverslip, the correct mounting medium preserves your histochemical stains or precipitable enzyme substrates in tissue sections or cell preparations.

Maximum retention of signal intensity is crucial for visualizing and imaging fluorescently stained target antigens in cell and tissue specimens. VECTASHIELD Antifade Mounting Media inhibit fading and photobleaching of most fluorophores, dyes, fluorescent proteins, and stains.


Resources

Immunohistochemistry Resource Guide

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
Learn more about this guide >

Immunofluorescence Resource Guide

Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents.  This guide will help you:

  • Learn about the IF workflow and how to optimize each step
  • Quickly identify and choose the most appropriate fluorescent secondary conjugates
  • Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving

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