Enzyme Substrates

Proprietary, easy-to-use enzyme substrates and kits

Vector's unique Horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme substrates offer:

  • Novel color choices — stain antigens in pigmented tissues and melanomas, or localize multiple antigens in a single tissue section
  • Visibility under brightfield, darkfield, or electron microscopy
  • Unique spectral profiles — useful for antigen colocalization using spectral imaging systems

Our enzyme substrate kits supply the reagents in convenient dropper bottles. This allows you to handle chromogens safely and easily, and eliminates wait times for mixing and dissolving powders or tablets.

Enzyme Substrates / Microscopy Table

This table is designed as a reference to help you determine the optimal mounting medium for your application.

   

Type of Microscopy

Substrate Catalog Number Brightfield Darkfield Electron Fluorescence Spectral
Imaging

Horseradish Peroxidase (HRP)

DAB (brown)

SK-4100

 

DAB-Ni (gray-black)

SK-4100

 

ImmPACT DAB (brown)

SK-4105

 

ImmPACT DAB EqV (brown)

SK-4103

 

Vector VIP (purple)

SK-4600

 

ImmPACT VIP (purple)

SK-4605

 

SG (blue-gray)

SK-4700

 

ImmPACT SG (blue-gray)

SK-4705

 

Vector NovaRED (red)

SK-4800

 

ImmPACT NovaRED (red)

SK-4805

 

AEC (red)

SK-4200

 

 

 

ImmPACT AEC (red)

SK-4205

 

 

 

ImmPACT AMEC Red (red)

SK-4285

 

 

 

TMB (blue)

SK-4400

 

 

 

Alkaline Phosphatase (AP)

Vector Red (magenta)

SK-5100

 

 

ImmPACT Vector Red (magenta)

SK-5105

 

 

Vector Black (black)

SK-5200

 

 

 

 

Vector Blue (blue)

SK-5300

 

 

BCIP / NBT (indigo)

SK-5400

 

 

Additional Enzyme Substrate Characteristics

This table is designed as a reference to help you determine the optimal mounting medium for your application.

Substrate Catalog Number Mounting
Medium
Contrast in
Pigmented
Tissue
Multiple
Labelling
Heat
Resistant

Horseradish Peroxidase (HRP)

DAB (brown)

SK-4100

Non-aqueous
or Aqueous

 

DAB-Ni (gray-black)

SK-4100

Non-aqueous

 

 

ImmPACT DAB (brown)

SK-4105

Non-aqueous
or Aqueous

 

ImmPACT DAB EqV (brown)

SK-4103

Non-aqueous
or Aqueous

 

Vector VIP (purple)

SK-4600

Non-aqueous

 

ImmPACT VIP (purple)

SK-4605

Non-aqueous

 

SG (blue-gray)

SK-4700

Non-aqueous
or Aqueous

 

ImmPACT SG (blue-gray)

SK-4705

Non-aqueous
or Aqueous

 

Vector NovaRED (red)

SK-4800

Non-aqueous

 

ImmPACT NovaRED (red)

SK-4805

Non-aqueous

 

AEC (red)

SK-4200

Aqueous

 

ImmPACT AEC (red)

SK-4205

Aqueous

 

ImmPACT AMEC Red (red)

SK-4285

Aqueous

 

TMB (blue)

SK-4400

Non-Aqueous

 

Alkaline Phosphatase (AP)

Vector Red (magenta)

SK-5100

Non-Aqueous
or Aqueous

ImmPACT Vector Red (magenta)

SK-5105

Non-Aqueous
or Aqueous

Vector Black (black)

SK-5200

Non-Aqueous

 

 

 

Vector Blue (blue)

SK-5300

Non-Aqueous
or Aqueous

BCIP / NBT (indigo)

SK-5400

Non-Aqueous
or Aqueous

 


Explore Enzyme Substrates (Chromogens)

Vector Labs offers alkaline phosphatase (AP) substrates suitable for several applications including cell and tissue based staining (IHC, IF, ISH), ELISA's and blotting.

Vector Labs offers peroxidase (HRP) substrates suitable for several applications including cell and tissue based staining (IHC, ISH) and blotting.


Resources

Immunohistochemistry Resource Guide

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
Learn more about this guide >

IHC Multiplexing Guide

A valuable resource for those considering localizing two or more antigens in the same tissue section. Protocols for chromogenic detection are accompanied by schematic illustrations of the procedures.
A chart of all pair-wise combinations of substrates is included along with photographic examples. Practical tips regarding order of substrates, blocking, and proper controls are also provided.

Enzyme Substrates Brochure

In addition to photomicrographs that help the user visualize the color that is generally produced
by each substrate, this brochure contains information on substrate properties that are especially useful for those who wish to multiplex (or introduce multiple labels in the same tissue section): A table of substrate properties, a schematic of relative senstitivities of substrate formulations, and a table of pairwise substrate compatibility are also included.

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