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Vector® Blue Substrate Kit, Alkaline Phosphatase (AP)

SK-5300
SKU Unit Size Price Qty
SK-5300 1 kit
$196.00
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Description

The Vector Blue AP Substrate Kit produces a blue, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Fluorescent
  • Heat Stable
  • Permanent mounting
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 200 ml of working solution
  • Excitation: broad, peak at ~500 nm*
  • Emission: broad, peak at ~680 nm*

*Histochem J. (1998) 8:577

Specifications

More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Alkaline Phosphatase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Blue
Color of Fluorescence Far Red
Microscopy Brightfield, Fluorescence, Spectral Imaging
Additional Substrate Properties Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)
Safety Title WARNING: Reproductive Harm - www.P65Warnings.ca.gov

Kit Components

Kit Contents:

  • 4 ml Vector Blue Reagent 1
  • 4 ml Vector Blue Reagent 2
  • 3 ml Vector Blue Reagent 3

Documents

Related Products

Endogenous alkaline phosphatase (AP) and peroxidase (HRP) activities in frozen intestine revealed with Vector Red AP Substrate (red) and ImmPACT DAB HRP Substrate (brown) (left). Same substrates used on BLOXALL Solution-treated tissue (right). BLOXALL B BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase Catalog #: SP-6000-100
Tumor tissue section showing specific cytoplasmic cell staining (blue/gray, Vector SG) with Vector Nuclear Fast Red (red) counterstain. Nuclear Fast Red Counterstain Catalog #: H-3403-500
Tonsil section showing specific cell staining (purple, Vector VIP) with Vector Methyl Green (green) counterstain. Methyl Green Counterstain Catalog #: H-3402-500
Prostate: Smooth muscle actin (m), ImmPRESS Anti-Mouse Ig Kit, AEC Substrate Kit (red). Hematoxylin QS counterstain (blue). Mounted in VectaMount AQ Mounting Medium. VectaMount® AQ Aqueous Mounting Medium Catalog #: H-5501-60
Levamisole Solution Levamisole Solution Catalog #: SP-5000-18

Citations

Technical Information

Vector Blue is both chromogenic and fluorescent. Sections stained with the Vector Blue substrate can be dehydrated, cleared, and permanently mounted; however, non-xylene clearing agents and non-xylene based mounting media (such as VectaMount®, H-5000) must be used because the reaction product is soluble in xylene. Vector Blue can also be used on blots in applications such as ELISPOT, singly or in combination with a peroxidase substrate such as AEC. This kit contains stock solutions in convenient dropper bottles.


 

 

 

 

Home Enzyme Substrates (Chromogens) Alkaline Phosphatase (AP) Substrates Vector® Blue Substrate Kit, Alkaline Phosphatase (AP)