ImmPACT® DAB EqV Peroxidase (HRP) Substrate

SKU Unit Size Price Qty
SK-4103-100 100 ml
SK-4103-400 400 ml

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ImmPACT DAB EqV consists of equal volumes of Reagent 1 and Reagent 2, and when mixed together form a working solution. ImmPACT DAB EqV produces a dark brown reaction product and is 3 to 5 times more sensitive than the original DAB substrate.


  • Optimized for IHC/ICC/ISH
  • Heat Stable
  • Suitable for permanent mounting


More Information
Unit Size 100 ml, 400 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Brown
Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate Properties Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)


Technical Information

DAB (3,3'-diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications.The DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted. The DAB reaction product can be intensified with DAB Enhancing Solution (H-2200).

The working solution is stable for several days when stored at 4°C. This substrate can be used for both manual and automated staining methods. ImmPACT DAB EqV can be used for blot detection on nylon and PVDF membranes but is not recommended for nitrocellulose membranes.

Relative sensitivity of immunohistochemistry (IHC) substrates

IHC counterstain / substrate compatibility table

Recommended alkaline phosphatase and peroxidase enzyme substrate combinations

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