Substrates

Vector Laboratories is a recognized leader in enzyme substrate technologies for protein and nucleic acid detection across a variety of applications, including immunohistochemistry (IHC), in situ hybridization (ISH), ELISAs, blotting, and more. Offering a portfolio of 18 ready-to-use enzyme substrate kits—13 for horseradish peroxidase (HRP) and 5 for alkaline phosphatase (AP)—Vector delivers a broad range of colorimetric options with distinct spectral profiles, enabling single and multiplex antigen labeling in brightfield, fluorescence, darkfield, and electron microscopy. These substrates support 9 unique color tones and 42 double-staining combinations, enhancing both sensitivity and visualization. Designed for optimal performance even in challenging samples such as pigmented tissues and those requiring heat-induced retrieval, these proprietary substrates come in convenient dropper bottles to streamline workflow and minimize handling errors. Substrate selection is pivotal to staining quality, and Vector’s enzyme substrates are engineered to offer unmatched clarity, reproducibility, and versatility.