Substrates

Substrates are chemical compounds used in enzyme-based detection methods to produce a visible or measurable signal upon reaction with enzyme-conjugated antibodies or probes. In protein detection, common enzymes include horseradish peroxidase (HRP) and alkaline phosphatase (AP), which catalyze substrate conversion to generate colorimetric, chemiluminescent, or fluorescent signals. The choice of substrate affects sensitivity, signal duration, and detection method compatibility. High-quality substrates are critical for clear, specific protein visualization. Vector Labs provides a variety of substrates for horseradish peroxidase (HRP) and alkaline phosphatase (AP), enabling colorimetric, chemiluminescent, and fluorescent detection in applications such as Immunohistochemistry, Western Blotting, and ELISA with high sensitivity and low background.