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Vector® SG Substrate Kit, Peroxidase (HRP) (SK-4700)

Purchase this product from 2BScientific
Colon Carcinoma: AE1/AE3 (m), ImmPRESS Reagent (HRP), Vector SG Substrate (gray).
Tumor (double label): p53 protein (m), VECTASTAIN ABC-AP Kit, Vector Red substrate (red); Pan-Cytokeratin (m), VECTASTAIN Elite ABC Kit, Vector SG Substrate (blue/gray).
Corticostriatal fibers of rat brain stained using Vector SG substrate viewed under darkfield microscopy.
Colon Carcinoma (double label): S100 (rp), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red); Cytokeratin 8/18 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Small Bowel (double label): Ki67 (rp), ImmPRESS Universal Reagent, Vector NovaRED substrate (red); Cytokeratin 8/18 (m), ImmPRESS Universal Reagent, Vector SG substrate (blue/gray).
Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, Vector SG substrate (blue/gray).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); CD34 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Tonsil (double label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector DAB substrate (brown); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Tonsil (double label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Tonsil (double label): CD3 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector VIP substrate (purple); Ki67 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector SG substrate (blue/gray).
Small bowel (double label): Neurofilament 200kD (m), ImmPRESS Anti-Mouse Ig Reagent, Vector VIP substrate (purple); Desmin (m), ImmPRESS Anti-Mouse Ig Reagent, Vector SG substrate (blue/gray).
Tonsil (triple label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, DAB (brown); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); Multi-cytokeratin (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Melanoma was stained with anti-vimentin followed by ImmPRESS™ HRP Anti-Rabbit IgG Reagent and Vector SG Substrate. Note the excellent contrast of the substrate with the brown pigments.
Tonsil (triple label): T cells (brown, Vector DAB), epithelium (purple, Vector VIP), B cells (blue/gray, Vector SG).
Intestine (double label): Desmin (brown, ImmPACT DAB). Cytokeratin (blue/gray, Vector SG).
Vector® SG Substrate Kit, Peroxidase (HRP)
Colon Carcinoma: AE1/AE3 (m), ImmPRESS Reagent (HRP), Vector SG Substrate (gray).
Tumor (double label): p53 protein (m), VECTASTAIN ABC-AP Kit, Vector Red substrate (red); Pan-Cytokeratin (m), VECTASTAIN Elite ABC Kit, Vector SG Substrate (blue/gray).
Corticostriatal fibers of rat brain stained using Vector SG substrate viewed under darkfield microscopy.
Colon Carcinoma (double label): S100 (rp), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red); Cytokeratin 8/18 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Small Bowel (double label): Ki67 (rp), ImmPRESS Universal Reagent, Vector NovaRED substrate (red); Cytokeratin 8/18 (m), ImmPRESS Universal Reagent, Vector SG substrate (blue/gray).
Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, Vector SG substrate (blue/gray).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); CD34 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Tonsil (double label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector DAB substrate (brown); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Tonsil (double label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Tonsil (double label): CD3 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector VIP substrate (purple); Ki67 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector SG substrate (blue/gray).
Small bowel (double label): Neurofilament 200kD (m), ImmPRESS Anti-Mouse Ig Reagent, Vector VIP substrate (purple); Desmin (m), ImmPRESS Anti-Mouse Ig Reagent, Vector SG substrate (blue/gray).
Tonsil (triple label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, DAB (brown); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); Multi-cytokeratin (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Melanoma was stained with anti-vimentin followed by ImmPRESS™ HRP Anti-Rabbit IgG Reagent and Vector SG Substrate. Note the excellent contrast of the substrate with the brown pigments.
Tonsil (triple label): T cells (brown, Vector DAB), epithelium (purple, Vector VIP), B cells (blue/gray, Vector SG).
Intestine (double label): Desmin (brown, ImmPACT DAB). Cytokeratin (blue/gray, Vector SG).
Vector® SG Substrate Kit, Peroxidase (HRP)

Description

The Vector SG HRP substrate produces a blue-gray reaction product that can be used as a single label or as a second color for multiple antigen labeling.

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Permanent mounting
  • No wait times for mixing and dissolving powders or tablets

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s)Peroxidase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorBlue-Gray
MicroscopyBrightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate PropertiesContrast with Tissue Pigments, Multiple Labeling
MountingAqueous (Hardening), Non-Aqueous (Permanent)

Kit Contents:

  • 5 ml SG Reagent 1
  • 7.2 ml SG Reagent 2

Documents

Citations

Technical Information

Sections stained with the substrate also can be viewed by darkfield or electron microscopy. Vector SG and ImmPACT SG substrates can be used for both manual and automated staining methods. Stained sections can be dehydrated, cleared, and permanently mounted.

Vector SG Substrate Kit contains stock solutions in convenient dropper bottles. The kit provides all of the necessary reagents to prepare about 300 ml of working solution.

 

 

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