The Vector Blue AP Substrate Kit produces a blue, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.
*Histochem J. (1998) 8:577
|Unit Size||1 kit|
|Applications||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot|
|Detection Enzyme(s)||Alkaline Phosphatase|
|Substrate Reaction Product||Precipitating Reaction Product|
|Color of Fluorescence||Far Red|
|Microscopy||Brightfield, Fluorescence, Spectral Imaging|
|Additional Substrate Properties||Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling|
|Mounting||Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)|
|Safety Title||WARNING: Reproductive Harm - www.P65Warnings.ca.gov|
Vector Blue is both chromogenic and fluorescent. Sections stained with the Vector Blue substrate can be dehydrated, cleared, and permanently mounted; however, non-xylene clearing agents and non-xylene based mounting media (such as VectaMount®, H-5000) must be used because the reaction product is soluble in xylene. Vector Blue can also be used on blots in applications such as ELISPOT, singly or in combination with a peroxidase substrate such as AEC. This kit contains stock solutions in convenient dropper bottles.
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