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Vector® Red Substrate Kit, Alkaline Phosphatase (AP)

SK-5100
SKU Unit Size Price Qty
SK-5100 1 kit
$195.00
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Description

Vector® Red AP Substrate Kit produces a magenta heat stable reaction product that is also fluorescent. Sections stained with the Vector® Red substrate can be dehydrated, cleared, and permanently mounted.

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Ideal for single and multiple labeling
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • One year expiry date
  • Sufficient reagents to produce 200 ml of working solution
  • Excitation: broad excitation spectrum, approx. 365 nm to 560 nm
  • Emission: Above 560 nm
  • Color: Red

Specifications

More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Alkaline Phosphatase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Magenta
Color of Fluorescence Red
Microscopy Brightfield, Fluorescence, Spectral Imaging
Additional Substrate Properties Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)

Kit Components

Kit Contents:

  • 4 ml Vector Red Reagent 1
  • 4 ml Vector Red Reagent 2
  • 4 ml Vector Red Reagent 3

Documents

Product FAQs

I have Vector Red AP substrate kit, SK-5100. The instructions for use suggest making a working solution with 100-200 mM Tris buffer. Can I use a 50 mM Tris buffer instead?

Vector Red substrate kit (SK-5100) does require a Tris buffer with a minimum of 100 mM to be present for a reaction to satisfactorily occur. It is recommended to use a 200 mM Tris buffer at pH 8.2-8.5 for the most optimal staining results. If a buffer with <100 mM Tris is used to make up the Vector Red working solution it may be that no reaction will occur.

Related Products

Endogenous alkaline phosphatase (AP) and peroxidase (HRP) activities in frozen intestine revealed with Vector Red AP Substrate (red) and ImmPACT DAB HRP Substrate (brown) (left). Same substrates used on BLOXALL Solution-treated tissue (right). BLOXALL B BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase Catalog #: SP-6000-100
Tonsil: Cytokeratin (m), ImmPRESS Anti-Mouse Ig Kit, ImmPACT DAB (brown) substrate. Hematoxylin (blue) counterstain. Hematoxylin Counterstain Catalog #: H-3401-500
Tonsil section showing specific cell staining (purple, Vector VIP) with Vector Methyl Green (green) counterstain. Methyl Green Counterstain Catalog #: H-3402-500
Small Bowel: Cytokeratin 8/18 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown), Hematoxylin QS (blue). Hematoxylin QS Counterstain Catalog #: H-3404-100
Prostate: Smooth muscle actin (m), ImmPRESS Anti-Mouse Ig Kit, AEC Substrate Kit (red). Hematoxylin QS counterstain (blue). Mounted in VectaMount AQ Mounting Medium. VectaMount® AQ Aqueous Mounting Medium Catalog #: H-5501-60
3D STORM imaging of Alexa Fluor 647 stained microtubules in a VECTASHIELD Mounting Medium/TRIS-Glycerol mixture. (Image kindly supplied by Dr. Nicolas Olivier, King's College, London UK.) VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10
Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd. VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10
Prostate: Prostate Specific Antigen (rp), fluorescein labeled using the ProtOn Fluorescein Labeling Kit. Propidium Iodide counterstain (red). Mounted in VECTASHIELD HardSet Mounting Medium. VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.* VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
Levamisole Solution Levamisole Solution Catalog #: SP-5000-18

Citations

Technical Information

This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. Because of its stability under ISH conditions, Vector® Red can be used in IHC/ISH double labeling applications. 

The bright red to orange fluorescence of the Vector Red reaction product can be visualized using DyLight™ 594, Texas Red® or TRITC filter systems. This fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes. This substrate can also be used for in situ hybridization or on blots, where it has a wider dynamic range than other substrates.

This kit contains stock solutions in convenient dropper bottles.


 

 

 

 

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