Vector® Red AP Substrate Kit produces a magenta heat stable reaction product that is also fluorescent. Sections stained with the Vector® Red substrate can be dehydrated, cleared, and permanently mounted.
Greater sensitivity than conventional substrates
Consistent and reliable
Ideal for IHC/ICC/ISH and blots
Ideal for single and multiple labeling
Stock solutions supplied in convenient dropper bottles promoting ease of handling
No wait times for mixing and dissolving powders or tablets
One year expiry date
Sufficient reagents to produce 200 ml of working solution
Excitation: broad excitation spectrum, approx. 365 nm to 560 nm
Emission: Above 560 nm
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Substrate Reaction Product
Precipitating Reaction Product
Color of Fluorescence
Brightfield, Fluorescence, Spectral Imaging
Additional Substrate Properties
Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
I have Vector Red AP substrate kit, SK-5100. The instructions for use suggest making a working solution with 100-200 mM Tris buffer. Can I use a 50 mM Tris buffer instead?
Vector Red substrate kit (SK-5100) does require a Tris buffer with a minimum of 100 mM to be present for a reaction to satisfactorily occur. It is recommended to use a 200 mM Tris buffer at pH 8.2-8.5 for the most optimal staining results. If a buffer with <100 mM Tris is used to make up the Vector Red working solution it may be that no reaction will occur.
This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. Because of its stability under ISH conditions, Vector® Red can be used in IHC/ISH double labeling applications.
The bright red to orange fluorescence of the Vector Red reaction product can be visualized using DyLight™ 594, Texas Red® or TRITC filter systems. This fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes. This substrate can also be used for in situ hybridization or on blots, where it has a wider dynamic range than other substrates.
This kit contains stock solutions in convenient dropper bottles.