The ImmPACT VIP HRP substrate produces an intense, violet (purple) reaction product, and can be used as an alternative to DAB or as a second color for multiple antigen labeling. This product generates an intense reaction product that is 2-4 times more sensitive than the Vector VIP substrate kit (SK-4600).
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) | Peroxidase |
| Substrate Reaction Product | Precipitating Reaction Product |
| Chromogen Color | Purple |
| Microscopy | Brightfield, Darkfield, Electron, Spectral Imaging |
| Additional Substrate Properties | Contrast with Tissue Pigments, Multiple Labeling |
| Mounting | Non-Aqueous (Permanent) |
BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase
Catalog #:
SP-6000-100
Methyl Green Counterstain
Catalog #:
H-3402-500
Hematoxylin Counterstain
Catalog #:
H-3401-500
Hematoxylin QS Counterstain
Catalog #:
H-3404-100
Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.
This kit consists of 120 ml of diluent and concentrated stock solutions of ImmPACT VIP reagents in convenient dropper bottles. Working solutions are stable for up to 14 days at 4 °C.
Product review on Biocompare.com.
1. Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122
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