ImmPACT® VIP Substrate Kit, Peroxidase (HRP)

SKU Unit Size Price Qty
SK-4605 1 kit
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The ImmPACT VIP HRP substrate produces an intense, violet (purple) reaction product, and can be used as an alternative to DAB or as a second color for multiple antigen labeling. This product generates an intense reaction product that is 2-4 times more sensitive than the Vector VIP substrate kit (SK-4600).


  • 2-4 fold greater sensitivity than original substrate kit
  • Optimized for IHC/ICC/ISH and blots
  • Permanent mounting
  • Ideal for single and multiple labeling
  • Working solutions stable for up to 14 days at 4 °C
  • Supplied as 120 ml of diluent and stock solutions in dropper bottles



More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Purple
Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate Properties Contrast with Tissue Pigments, Multiple Labeling
Mounting Non-Aqueous (Permanent)

Kit Components

Kit Contents:

  • 120 ml ImmPACT VIP Diluent
  • 3 ml ImmPACT VIP Reagent 1
  • 2.5 ml ImmPACT VIP Reagent 2
  • 2.5 ml ImmPACT VIP Reagent 3
  • 3 ml ImmPACT VIP Reagent 4



Technical Information

Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.

This kit consists of 120 ml of diluent and concentrated stock solutions of ImmPACT VIP reagents in convenient dropper bottles. Working solutions are stable for up to 14 days at 4 °C.

Product review on

1.  Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122





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