ImmPACT Vector Red AP Substrate Kit produces a magenta reaction product that is crisper and is 2-4 times more sensitive than the original Vector® Red substrate kit (SK-5100). It can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.
Ideal for IHC/ICC/ISH and blots
Supplied as 120 ml of ready-to-use diluent and stock solutions in dropper bottles
Excitation: broad excitation spectrum, approx. 365 nm to 560 nm
Emission: Above 560 nm
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Substrate Reaction Product
Precipitating Reaction Product
Color of Fluorescence
Brightfield, Fluorescence, Spectral Imaging
Additional Substrate Properties
Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
Both Vector Red and ImmPACT Vector Red alkaline phosphatase (AP) substrates produce red, heat stable reaction products that are also highly fluorescent and non-fading. Preparations stained with either Vector Red or ImmPACT Vector Red AP chromogen can be dehydrated, cleared, and permanently mounted. Because of their stability under ISH conditions, both chromogens can be used in IHC/ISH double labeling applications. Both formulations can be used for either manual or automated staining methods.
The bright fluorescence of the Vector Red or ImmPACT Vector Red reaction products are visible using DyLight™ 594, Texas Red® or TRITC filter systems. The fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes.
Either substrate formulation can also be used on blots. Compared to other AP substrates, the Vector Red and ImmPACT Vector Red reaction products have a wider dynamic range, offering better quantitation of targets on blots.