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ImmPACT® Vector® Red Substrate Kit, Alkaline Phosphatase (AP)

SK-5105
SKU Unit Size Price Qty
SK-5105 1 kit
$168.00
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Description

ImmPACT Vector Red AP Substrate Kit produces a magenta reaction product that is crisper and is 2-4 times more sensitive than the original Vector® Red substrate kit (SK-5100). It can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.

Features:

  • Ideal for IHC/ICC/ISH and blots
  • Fluorescent
  • Heat Stable
  • Permanent mounting
  • Supplied as 120 ml of ready-to-use diluent and stock solutions in dropper bottles
  • Excitation: broad excitation spectrum, approx. 365 nm to 560 nm
  • Emission: Above 560 nm
  • Color: Red

Specifications

More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Alkaline Phosphatase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Magenta
Color of Fluorescence Red
Microscopy Brightfield, Fluorescence, Spectral Imaging
Additional Substrate Properties Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)

Kit Components

Kit Contents:

  • 120 ml ImmPACT Vector Red Diluent
  • 2.5 ml ImmPACT Vector Red Reagent 1
  • 2.5 ml ImmPACT Vector Red Reagent 2

Documents

Related Products

Endogenous alkaline phosphatase (AP) and peroxidase (HRP) activities in frozen intestine revealed with Vector Red AP Substrate (red) and ImmPACT DAB HRP Substrate (brown) (left). Same substrates used on BLOXALL Solution-treated tissue (right). BLOXALL B BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase Catalog #: SP-6000-100
Tonsil: Cytokeratin (m), ImmPRESS Anti-Mouse Ig Kit, ImmPACT DAB (brown) substrate. Hematoxylin (blue) counterstain. Hematoxylin Counterstain Catalog #: H-3401-500
Small Bowel: Cytokeratin 8/18 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown), Hematoxylin QS (blue). Hematoxylin QS Counterstain Catalog #: H-3404-100
Tonsil section showing specific cell staining (purple, Vector VIP) with Vector Methyl Green (green) counterstain. Methyl Green Counterstain Catalog #: H-3402-500
Prostate: Prostate Specific Antigen (rp), fluorescein labeled using the ProtOn Fluorescein Labeling Kit. Propidium Iodide counterstain (red). Mounted in VECTASHIELD HardSet Mounting Medium. VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.* VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
Prostate: Smooth muscle actin (m), ImmPRESS Anti-Mouse Ig Kit, AEC Substrate Kit (red). Hematoxylin QS counterstain (blue). Mounted in VectaMount AQ Mounting Medium. VectaMount® AQ Aqueous Mounting Medium Catalog #: H-5501-60
Levamisole Solution Levamisole Solution Catalog #: SP-5000-18
Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd. VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10
3D STORM imaging of Alexa Fluor 647 stained microtubules in a VECTASHIELD Mounting Medium/TRIS-Glycerol mixture. (Image kindly supplied by Dr. Nicolas Olivier, King's College, London UK.) VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10

Citations

Technical Information

Both Vector Red and ImmPACT Vector Red alkaline phosphatase (AP) substrates produce red, heat stable reaction products that are also highly fluorescent and non-fading. Preparations stained with either Vector Red or ImmPACT Vector Red AP chromogen can be dehydrated, cleared, and permanently mounted. Because of their stability under ISH conditions, both chromogens can be used in IHC/ISH double labeling applications. Both formulations can be used for either manual or automated staining methods.

The bright fluorescence of the Vector Red or ImmPACT Vector Red reaction products are visible using DyLight™ 594, Texas Red® or TRITC filter systems. The fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes.

Either substrate formulation can also be used on blots. Compared to other AP substrates, the Vector Red and ImmPACT Vector Red reaction products have a wider dynamic range, offering better quantitation of targets on blots.


 

 

 

 

 

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