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BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium)

SK-5400
SKU Unit Size Price Qty
SK-5400 1 kit
$184.00
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Description

BCIP/NBT AP Substrate Kit forms an indigo, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Fluorescent
  • Heat Stable
  • Permanent mounting
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 200 ml of working solution
  • Excitation: 645-685 nm*
  • Emission: peaks at 823 and 855 nm*

*Biotechniques (2007) 42:756-759 and BioTechniques (2014) 57:254-256

 

Specifications

More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Alkaline Phosphatase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Indigo
Color of Fluorescence Far Red
Microscopy Brightfield, Fluorescence, Spectral Imaging
Additional Substrate Properties Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)
Safety Title WARNING: Reproductive Harm - www.P65Warnings.ca.gov

Kit Components

Kit Contents:

  • 4 ml BCIP/NBT Reagent 1
  • 3 ml BCIP/NBT Reagent 2
  • 4 ml BCIP/NBT Reagent 3

Documents

Related Products

Endogenous alkaline phosphatase (AP) and peroxidase (HRP) activities in frozen intestine revealed with Vector Red AP Substrate (red) and ImmPACT DAB HRP Substrate (brown) (left). Same substrates used on BLOXALL Solution-treated tissue (right). BLOXALL B BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase Catalog #: SP-6000-100
Tumor tissue section showing specific cytoplasmic cell staining (blue/gray, Vector SG) with Vector Nuclear Fast Red (red) counterstain. Nuclear Fast Red Counterstain Catalog #: H-3403-500
Tonsil section showing specific cell staining (purple, Vector VIP) with Vector Methyl Green (green) counterstain. Methyl Green Counterstain Catalog #: H-3402-500
Prostate: Smooth muscle actin (m), ImmPRESS Anti-Mouse Ig Kit, AEC Substrate Kit (red). Hematoxylin QS counterstain (blue). Mounted in VectaMount AQ Mounting Medium. VectaMount® AQ Aqueous Mounting Medium Catalog #: H-5501-60
Levamisole Solution Levamisole Solution Catalog #: SP-5000-18

Citations

Technical Information

This substrate can be used for developing blots or for in situ hybridization. At 20-30 minute development times, the sensitivity of this substrate is equivalent to the other alkaline phosphatase substrates. However, BCIP/NBT will continue to develop from several hours to overnight making it one of the most sensitive chromogenic substrates. This kit contains stock solutions in convenient dropper bottles.


 

 

 

 

Home Enzyme Substrates (Chromogens) Alkaline Phosphatase (AP) Substrates BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium)