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Vector® VIP Substrate Kit, Peroxidase (HRP) (SK-4600)

Small Bowel: Neurofilament 200kD (m), ImmPRESS Anti-Mouse Ig Kit, Vector VIP Substrate Kit (purple).
Tonsil triple stained using the VECTASTAIN Universal Elite ABC Kit: T cells (brown, DAB), B cells (purple, Vector VIP), epithelium (blue/gray, Vector SG).
Vector® VIP Substrate Kit, Peroxidase (HRP)
Breast Carcinoma (double label): ki67 (brown, Vector DAB). Endothelium (purple, Vector VIP).
Tonsil (double label): T cells (purple, Vector VIP). Ki67 (gray/back, DAB/Ni).
Tonsil (triple label): T cells (brown, Vector DAB), epithelium (purple, Vector VIP), B cells (blue/gray, Vector SG).
Melanoma was stained with anti-vimentin followed by ImmPRESS™ HRP Anti-Rabbit IgG Reagent and Vector VIP Substrate. Note the excellent contrast of the substrate with the brown pigments.
Tonsil (triple label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, DAB (brown); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); Multi-cytokeratin (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Small bowel (double label): Neurofilament 200kD (m), ImmPRESS Anti-Mouse Ig Reagent, Vector VIP substrate (purple); Desmin (m), ImmPRESS Anti-Mouse Ig Reagent, Vector SG substrate (blue/gray).
Breast Carcinoma (double label): Estrogen Receptor (rm), ImmPRESS Universal Reagent, DAB substrate (brown); M2A Antigen (m), ImmPRESS Universal Reagent, Vector VIP substrate (purple).
Tonsil (double label): CD3 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector VIP substrate (purple); Ki67 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector SG substrate (blue/gray).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); CD34 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Breast Carcinoma (double label): Estrogen Receptor (rm), ImmPRESS Universal Reagent, Vector DAB substrate (brown); M2A Antigen (m), ImmPRESS Universal Reagent, Vector VIP substrate (purple).
Breast Carcinoma (double label): Ki67 (rm), ImmPRESS Universal Reagent, DAB substrate (brown); CD34 (m), ImmPRESS Universal Reagent, Vector VIP substrate (purple).
Colon (double label): M2A antigen (m), VECTASTAIN ABC-AP Kit (Universal), Vector Blue (blue). CD20 (m), ImmPRESS Reagent (HRP, Universal), Vector VIP (purple).
EMMPRIN (CD147) Expression in human retina. Image kindly provided by Gail Seigel, University of Buffalo, Center for Hearing and Deafness.
Vector® VIP Substrate Kit, Peroxidase (HRP)
Small Bowel: Neurofilament 200kD (m), ImmPRESS Anti-Mouse Ig Kit, Vector VIP Substrate Kit (purple).
Tonsil triple stained using the VECTASTAIN Universal Elite ABC Kit: T cells (brown, DAB), B cells (purple, Vector VIP), epithelium (blue/gray, Vector SG).
Vector® VIP Substrate Kit, Peroxidase (HRP)
Breast Carcinoma (double label): ki67 (brown, Vector DAB). Endothelium (purple, Vector VIP).
Tonsil (double label): T cells (purple, Vector VIP). Ki67 (gray/back, DAB/Ni).
Tonsil (triple label): T cells (brown, Vector DAB), epithelium (purple, Vector VIP), B cells (blue/gray, Vector SG).
Melanoma was stained with anti-vimentin followed by ImmPRESS™ HRP Anti-Rabbit IgG Reagent and Vector VIP Substrate. Note the excellent contrast of the substrate with the brown pigments.
Tonsil (triple label): CD3 (m), VECTASTAIN Universal Elite ABC Kit, DAB (brown); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); Multi-cytokeratin (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Small bowel (double label): Neurofilament 200kD (m), ImmPRESS Anti-Mouse Ig Reagent, Vector VIP substrate (purple); Desmin (m), ImmPRESS Anti-Mouse Ig Reagent, Vector SG substrate (blue/gray).
Breast Carcinoma (double label): Estrogen Receptor (rm), ImmPRESS Universal Reagent, DAB substrate (brown); M2A Antigen (m), ImmPRESS Universal Reagent, Vector VIP substrate (purple).
Tonsil (double label): CD3 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector VIP substrate (purple); Ki67 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector SG substrate (blue/gray).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN Universal Elite ABC Kit, Vector VIP substrate (purple); CD34 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).
Breast Carcinoma (double label): Estrogen Receptor (rm), ImmPRESS Universal Reagent, Vector DAB substrate (brown); M2A Antigen (m), ImmPRESS Universal Reagent, Vector VIP substrate (purple).
Breast Carcinoma (double label): Ki67 (rm), ImmPRESS Universal Reagent, DAB substrate (brown); CD34 (m), ImmPRESS Universal Reagent, Vector VIP substrate (purple).
Colon (double label): M2A antigen (m), VECTASTAIN ABC-AP Kit (Universal), Vector Blue (blue). CD20 (m), ImmPRESS Reagent (HRP, Universal), Vector VIP (purple).
EMMPRIN (CD147) Expression in human retina. Image kindly provided by Gail Seigel, University of Buffalo, Center for Hearing and Deafness.
Vector® VIP Substrate Kit, Peroxidase (HRP)
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Description

The Vector VIP HRP substrate produces an intense, violet (purple) reaction product, and can be used as an alternative to DAB or as a second color for multiple antigen labeling.

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Permanent mounting
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 300 ml of working solution

Specifications

More Information
Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s)Peroxidase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorPurple
MicroscopyBrightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate PropertiesContrast with Tissue Pigments, Multiple Labeling
MountingNon-Aqueous (Permanent)

Kit Contents:

  • 7 ml Vector VIP Reagent 1
  • 5 ml Vector VIP Reagent 2
  • 5 ml Vector VIP Reagent 3
  • 7.2 ml Vector VIP Reagent 4

Documents

Citations

Technical Information

Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.

Vector VIP Substrate Kit contains stock solutions in convenient dropper bottles. The sensitivity of Vector VIP substrate is equivalent to Vector DAB Substrate. This product can also be used on blots. The kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4° C, this kit is stable for one year.

Product review on Biocompare.com.

1.  Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122

SUBSTRATEsensitivity2 jpg

Substrate.CounterstainChart jpg SubstrateCombos jpg

Applicable patents and legal notices are available at legal notices.

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