High-sensitivity avidin-biotin kits with peroxidase-based detection

Horseradish peroxidase (HRP) substrates produce sharp, dense precipitates with crisp localization. In conjunction with the high sensitivity and low background of VECTASTAIN ABC systems, HRP-based detection systems are a preferred choice for many applications, including immunohistochemistry (IHC), immunocytochemistry (ICC), protein and nucleic acid blot, in situ hybridization (ISH), and enzyme immunoassay (ELISA) detection.

  • Horseradish peroxidase (HRP) enzyme detection system
  • Avidin/biotin technology
  • High sensitivity/low background
  • Low cost
  • Used as a second label in multiple antigen labeling applications

Choose from among three different kit formats:

  • VECTASTAIN ELITE® ABC Kits  — our most sensitive formulations, these kits offer a smaller and more uniform avidin-biotin complex for greater accessibility to biotinylated targets within tissue samples; kits are available in concentrated formats, or as prediluted, ready-to-use (R.T.U.) formulations
  • VECTASTAIN Original ABC Kits — our original and most economical formulation, these kits offers good sensitivity with low background
  • VECTASTAIN Universal Quick Kits — our most rapid protocol, these kits offer a proprietary peroxidase-streptavidin pre-formed complex for outstanding sensitivity with short incubation times; used to quickly detect primary antibodies made in mouse, rabbit, or goat, these kits are available in concentrated formats, or as prediluted, ready-to-use (R.T.U.) formulations

When using the VECTASTAIN Original or ELITE ABC Kits:

  • To detect a biotinylated target, use the VECTASTAIN ABC Standard Kit with an appropriate substrate
  • To detect an unlabeled primary antibody or lectin, use a biotinylated secondary antibody (binds the primary antibody species or lectin you have chosen), VECTASTAIN ABC reagent, and appropriate substrate (for example, to detect a rabbit primary antibody, choose the VECTASTAIN ABC Kit, Rabbit IgG).
Breast Carcinoma: • Estrogen Receptor (m); VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red) • CD34 (m) • VECTASTAIN ELITE ABC Kit, DAB substrate (brown) • Cytokeratin 8/18 (m), VECTASTAIN ELITE ABC Kit, Vector SG Substrate (blue/gray)
Tonsil: • Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, Vector® DAB (brown) • CD3 (m), VECTASTAIN® Elite® ABC Kit, Vector® VIP (purple) • CD20 (m), VECTASTAIN® Elite® ABC Kit, Vector® SG (blue-gray).