The M.O.M. Basic Kit is useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts. The M.O.M. Kit can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems.
Unit Size | 1 kit |
---|---|
Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence |
Technology | ABC (Avidin/Biotin) Systems, Mouse on Mouse (M.O.M.) Systems |
Target Species | Mouse |
Format | Concentrate |
Blocking Action | Endogenous Mouse Ig |
Kit Contents:
Vector Laboratories’ Mouse on Mouse detection kits (M.O.M.®) are designed for the detection of mouse IgG primary antibodies on mouse tissue sections. For detecting mouse primary antibodies on rat tissue, we offer a selection of anti-mouse, rat adsorbed secondary detection reagents. These are intended to be used for this application and would generate the most optimal signal to noise staining ratio. We offer a biotinylated anti-mouse IgG, rat adsorbed secondary antibody (Cat. No. BA-2001) for use with VECTASTAIN® ABC kits or avidin and streptavidin enzyme conjugates. Alternatively, we offer an ImmPRESS® HRP polymer anti-mouse IgG, rat adsorbed detection kit (Cat. No. MP-7422) for a convenient, one-step IHC methodology.
Four M.O.M. Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M. Mouse Ig Blocking Reagent and the M.O.M. Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M. Mouse Ig Blocking Reagent and M.O.M. Biotinylated Anti-Mouse Ig Reagent are also available separately.
The M.O.M. ImmPRESS® Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M. ImmPRESS Peroxidase Polymer reagent.
An enzyme or fluorochrome conjugate is not included allowing for a choice of any avidin or streptavidin-based detection system. Choose any peroxidase or alkaline phosphatase conjugated avidin or streptavidin for chromogenic detection (for example, VECTASTAIN ABC-AP Standard Kit followed by a Vector alkaline phosphatase substrate), or choose a fluorochrome-labeled avidin or streptavidin.
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