SpeakEasy Science Blog

Multiplexing has revolutionized immunostaining workflows. It allows the simultaneous detection of multiple targets in the same tissue section and empowers scientists to gather more data while sparing precious samples. But issues with cross-reactivity between reagents can hinder the ability to perform simultaneous double or triple staining. When these issues arise, scientists can use the VectaPlexÔ Antibody Removal Kit to do multiple rounds of labeling and perform multiplexing experiments.

Creating a product that stands out and addresses the needs of scientists requires ingenuity, persistence, and a lot of testing; our new product, VectaPlex™ Antibody Removal Kit, is no exception. We sat down with Senior Scientist August Estabrook, PhD, to get an inside look into the birth and development of VectaPlex. 

Multiplexing, a powerful technique that allows the visualization of multiple biomarkers within a single sample, has revolutionized the field by offering researchers valuable insights into the spatial context of complex cellular processes and interactions. By analyzing multiple targets in the same specimen, researchers can obtain a holistic view of their tissue while conserving precious sample. However, performing multiplex assays can be challenging. Cross reactivity between detection reagents can often limit reagent choices and circumventing this by directly labeling primary antibodies can lead to altered antibody function and loss of specificity. Thankfully, VectaPlex™ Antibody Removal Kit comes to the rescue, simplifying the multiplex workflow, preserving tissue integrity, and enabling multiple rounds of staining without reducing target availability.