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One pain for IHC and IF staining can be the lack of positive staining on your tissue sections. In this blog post, we will offer tips and tricks for overcoming the absence of positive staining in IHC and IF.
In this post we will discuss common sources of background staining and offer tips and tricks for resolving these issues.
This blog post will walk you through strategies in four different categories to protect your immunofluorescent tissue section from photobleaching.
As far as antifade mounting media go, one-size-fits-all solutions are few and far between. Here, we break down key factors you should consider when choosing the best antifade mounting medium for your experiment.
Identifying sources of non-specific staining is crucial for determining potential solutions to improve your staining results. This blog post will briefly walk you through commonly encountered sources of non-specific staining in IHC and IF applications.
You spend a lot of time collecting and preparing your tissue samples. Learn tips and tricks to ensure those samples are evenly stained and retained on your slides.
You’ve got lots of options when it comes to choosing a mounting media. Let us help you decide whether setting or non-setting mounting media will work best for your workflow in this Tips & Tricks article.
Interfering background signal, such as autofluorescence, which is fluorescence inherent or endogenous to tissue, cell, or due to certain fixatives, can hamper your ability to accurately distinguish real target antigen expression. In a recent webinar, Dr. Craig Pow discussed common causes of target signal interference, controls to help distinguish background noise from true signal, ways to reduce or eliminate autofluorescence, case studies of autofluorescence quenching, and tips to preserve immunofluorescent signal, such as through the use of Vector® TrueVIEW® Autoflurorescence Quenching Kit. You can watch the full on-demand webinar or keep reading for tips and tricks from the Q&A session.
When it comes to IHC and IF a strong start is essential to great results. Explore our tips to help your sample preparation process.
Autofluorescence can make it difficult or impossible to distinguish antigen-specific signal from non-specific background. Learn how you can reduce autofluorescence in your immunofluorescence workflow.
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