Considerations When Selecting a Primary Antibody
With a wide variety to choose from, there are important considerations to make when selecting your own primary antibody. Keep reading to learn how to choose a primary antibody for your workflow.
Insights from our team
Vector Laboratories’ blog provides a wealth of information on immunohistochemistry (IHC), immunofluorescence (IF), and related life science research topics.
Immunofluorescence
5 reasons to use the ImmEdge PAP Pen in your immunostaining workflow
In this blog post, we cover 5 reasons you should add the ImmEdge® Hydrophobic Barrier PAP Pen from Vector Laboratories to your list of essential lab tools.
My staining didn’t work, part 3: IHC and IF staining tips
This blog will discuss some miscellaneous factors to focus on when an IHC or IF protocol performs unexpectedly.
My staining didn’t work, part 2: Absence of positive staining in IHC and IF
One pain for IHC and IF staining can be the lack of positive staining on your tissue sections. In this blog post, we will offer tips and tricks for overcoming the absence of positive staining... Read more
My staining didn’t work, part 1: Background staining in IHC and IF
In this post we will discuss common sources of background staining and offer tips and tricks for resolving these issues.
How to protect your tissue from photobleaching
This blog post will walk you through strategies in four different categories to protect your immunofluorescent tissue section from photobleaching.
How to choose antifade mounting media
As far as antifade mounting media go, one-size-fits-all solutions are few and far between. Here, we break down key factors you should consider when choosing the best antifade mounting medium for your experiment.
5 sources of non-specific staining and background in IHC and IF
Identifying sources of non-specific staining is crucial for determining potential solutions to improve your staining results. This blog post will briefly walk you through commonly encountered sources of non-specific staining in IHC and IF applications.
How to prevent tissue and reagent loss on your slides
You spend a lot of time collecting and preparing your tissue samples. Learn tips and tricks to ensure those samples are evenly stained and retained on your slides.
Setting media vs non-setting mounting media: Which is right for you?
You’ve got lots of options when it comes to choosing a mounting media. Let us help you decide whether setting or non-setting mounting media will work best for your workflow in this Tips & Tricks... Read more
