Vector Laboratories’ blog provides a wealth of information on immunohistochemistry (IHC), immunofluorescence (IF), and related life science research topics.
Interfering background signal, such as autofluorescence, which is fluorescence inherent or endogenous to tissue, cell, or due to certain fixatives, can hamper your ability to accurately distinguish real target antigen expression. In a recent webinar,... Read more
When it comes to IHC and IF a strong start is essential to great results. Explore our tips to help your sample preparation process.
Autofluorescence can make it difficult or impossible to distinguish antigen-specific signal from non-specific background. Learn how you can reduce autofluorescence in your immunofluorescence workflow.
No matter how you slice it, if your research includes immunohistochemistry or immunofluorescence, it’s a key part of your experimental workflow. If you aren’t the type of researcher who draws a heart around “Sectioning Day”... Read more
Looking for insights into how to choose the right mounting medium for your applications? From understanding the importance of refractive index to technique tips, we’ve got you covered.
When everything goes right, looking through the microscope at your immunofluorescence-stained tissue is no less beautiful than peering through a telescope at the night sky. Unfortunately, experimental issues can obscure that pristine view of your... Read more
There’s nothing worse than toiling away all week to repeat an experiment only to have your staining turn up blank. You’re left scratching your head trying to figure out why you’ve got nothing. Recently, Vector... Read more
Immunohistochemistry Immunofluorescence Immunohistochemistry (IHC) and immunofluorescence (IF) are widely used across the life sciences—in cancer research, immunology, neuroscience, cell biology, and histopathology—to help elucidate protein expression in cell and tissue specimens. And while these are... Read more
