How to choose antifade mounting media
As far as antifade mounting media go, one-size-fits-all solutions are few and far between. Here, we break down key factors you should consider when choosing the best antifade mounting medium for your experiment.
Insights from our team
Vector Laboratories’ blog provides a wealth of information on immunohistochemistry (IHC), immunofluorescence (IF), and related life science research topics.
Immunofluorescence
5 sources of non-specific staining and background in IHC and IF
Identifying sources of non-specific staining is crucial for determining potential solutions to improve your staining results. This blog post will briefly walk you through commonly encountered sources of non-specific staining in IHC and IF applications.
How to prevent tissue and reagent loss on your slides
You spend a lot of time collecting and preparing your tissue samples. Learn tips and tricks to ensure those samples are evenly stained and retained on your slides.
Setting media vs non-setting mounting media: Which is right for you?
You’ve got lots of options when it comes to choosing a mounting media. Let us help you decide whether setting or non-setting mounting media will work best for your workflow in this Tips & Tricks... Read more
How to improve your immunofluorescence by overcoming autofluorescence
Interfering background signal, such as autofluorescence, which is fluorescence inherent or endogenous to tissue, cell, or due to certain fixatives, can hamper your ability to accurately distinguish real target antigen expression. In a recent webinar,... Read more
Start Right to Finish Well—The Importance of the Sample Preparation Process in Your IHC and IF Workflow
When it comes to IHC and IF a strong start is essential to great results. Explore our tips to help your sample preparation process.
Reduce Tissue Autofluorescence and Dramatically Enhance Signal-to-Noise Ratio
Autofluorescence can make it difficult or impossible to distinguish antigen-specific signal from non-specific background. Learn how you can reduce autofluorescence in your immunofluorescence workflow.
Let’s Cut to the Chase: Tips and Tricks to Get the Most Out of Your Sectioning
No matter how you slice it, if your research includes immunohistochemistry or immunofluorescence, it’s a key part of your experimental workflow. If you aren’t the type of researcher who draws a heart around “Sectioning Day”... Read more
The End of the Line: Considerations for Mounting Media Selection
Looking for insights into how to choose the right mounting medium for your applications? From understanding the importance of refractive index to technique tips, we’ve got you covered.
Reduce TissueBetter Immunofluorescence at the Speed of Light
When everything goes right, looking through the microscope at your immunofluorescence-stained tissue is no less beautiful than peering through a telescope at the night sky. Unfortunately, experimental issues can obscure that pristine view of your... Read more
