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Immunohistochemistry Resource Guide

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IHC Guide Cover v1

Table of Contents

Counterstaining

A counterstain introduces color to specific cellular structures to provide contrast to the colored enzyme substrate. Counterstaining aids in visualization and target localization, facilitating interpretation of morphology and cell structure within the tissue section. Our nuclear counterstains are packaged as convenient, ready-to-use solutions for use on individual slides or in staining dishes.

Vector® Hematoxylin (blue)

  • Based on Gill’s III formulation
  • Progressive stain formula. The intensity can be adjusted to optimize results for either manual or automated systems
  • Excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
  • Suitable for use with non-aqueous and aqueous mounting media
  • Alcohol- and mercury-free
Tumor tissue counterstain blue
Tumor tissue section showing specific cytoplasmic cell staining (Vector DAB, brown) with Vector Hematoxylin QS counterstain (blue).

Vector Hematoxylin QS (blue)

  • Modification of Mayer’s hematoxylin developed especially forimmunocytochemistry
  • Ready-to-use without filtration or ‘blueing’ step
  • Stains in less than 45 seconds
  • Excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
  • Suitable for use with non-aqueous and aqueous mounting media
  • Mercury-free
H 3404CROP
Small Bowel: Cytokeratin 8/18 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown), Hematoxylin QS (blue).

Methyl Green (light green)

  • Superior formulation of methyl green suitable for use with a wide range of enzyme substrates
  • Simple, two step procedure
  • Excellent alternative in multiple antigen labeling when hematoxylin obscures the substrate colors
  • Suitable for use with non-aqueous mounting media
Tonsil counterstain green
Tonsil section showing specific cell staining (Vector VIP, purple) with Vector Methyl Green counterstain (green).

Vector Nuclear Fast Red (pink)

  • Fast one-step protocol
  • Excellent alternative in multiple antigen labeling when hematoxylin obscures the substrate colors
  • Good contrast with a variety of substrates
tumor counterstain red
Tumor tissue section showing specific cytoplasmic cell staining (Vector SG, blue/ gray) with Vector Nuclear Fast Red counterstain (red).