Immunohistochemistry Workflow

Immunohistochemistry Resource Guide

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Vector Laboratories is your resource for premium labeling and detection products at each step of your immunohistochemistry (IHC) workflow. Our IHC workflow provides useful reagents to improve your staining.

Tissue Preparation	• VECTABOND^® Tissue Section Adhesive • ImmPrint™ Histology Pen • ImmEdge^® Hydrophobic Barrier Pen
Antigen Retrieval	• Antigen Unmasking Solutions, Citrateor Tris-based
Quench / Block	• BLOXALL^® Endogenous HRP and AP Blocking Solution • Avidin/Biotin Blocking Kit • Streptavidin / Biotin Blocking Kit • Normal Sera • Animal-Free Block^® and Diluent • BSA • Casein Solution • Vector^® M.O.M.^® Mouse Ig Blocking Reagent • Carbo-Free™ Block
Primary Antibody / Lectins*	• Biotinylated lectins • Unconjugated lectins
Secondary Antibody	• ImmPRESS^® Polymer Reagents (HRP or AP) • M.O.M.^® (Mouse on Mouse) ImmPRESS^® Polymer Kit • M.O.M.^® (Mouse on Mouse) Basic Kit • Biotinylated secondary antibodies • Unconjugated secondary antibodies • Enzyme-conjugated secondary antibodies (HRP or AP) • Biotinylated anti-lectins
Tertiary Reagent	• VECTASTAIN^® ABC complex reagents • ImmPRESS^® Excel Amplified Staining Kits • M.O.M.^® (Mouse on Mouse) Elite^® Kit (HRP) • Enzyme-conjugated avidin/streptavidin (HRP or AP)
Substrate / Chromogen	• HRP substrates • AP substrates/ Levamisole Solution
Counterstain	• Hematoxylin • Methyl Green • Nuclear Fast Red
Coverslip / Mount	• VectaMount^® Mounting Medium • VectaMount^® AQ Mounting Medium
Visualize

* For more information about lectins used in an IHC workflow, visit: vectorlabs.com/lectins

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Immunohistochemistry Resource Guide

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Immunohistochemistry Workflow

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