Immunohistochemistry Resource Guide
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Table of Contents
Immunohistochemistry Selection Guide
Avidin-Biotin Complex (ABC)-Based Detection
Choosing a VECTASTAIN® ABC Kit
> Customizing your VECTASTAIN® ABC Kit
ImmPRESS® One-Step Polymer Systems
> ImmPRESS® Two-Step Amplified Polymer Systems
> Choosing an ImmPRESS® Polymer Kit
> Multiple Antigen Labeling Simplified
M.O.M.® (Mouse on Mouse) Immunodetection Kits
> Counterstain/Substrate Compatibility Table
Table of Contents
A key advantage of the ImmPRESS® Polymer Reagent is that it significantly shortens staining times for multiplex immunohistochemistry (IHC) protocols.
- Fewer steps than conventional protocols decreases slide handling.
- Eliminates the need for avidin/biotin blocking steps in samples with endogenous biotin.
Breast Carcinoma: • Ki67 (rm), ImmPRESS Reagent (HRP; Universal), Vector® DAB (brown) • CD34, ImmPRESS Reagent (HRP; Universal), Vector VIP (purple).
Colon: • M2A antigen (m), VECTASTAIN® ABC-AP Kit (Universal), Vector Blue (blue) • CD20 (m), ImmPRESS Reagent (HRP; Universal), Vector VIP (purple).
Colon: • CD3 (rm), ImmPRESS Reagent (HRP) Anti-Rabbit IgG, ImmPACT AMEC Red • CD34 (m), ImmPRESS-AP Anti-Mouse IgG Reagent, Vector Blue (blue).
Using the ImmPRESS® Polymer Kits for multiplex IHC protocols.
1 Add First Primary Antibody
2 Add ImmPRESS Reagent
3 Add Enzyme Substrate I
4 Add Second Primary Antibody
5 Add ImmPRESS Reagent
6 Add Enzyme Substrate II
Read more about multiplex IHC protocols: Discovery Through Color: IHC Multiplexing Guide
