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When choosing the optimal detection system for your application, consider not only the species of the primary antibody but also the species of the tissue under examination. If the species of the primary antibody and the species of the tissue are closely related (for example, rat and mouse), the secondary antibody may cross-react with endogenous IgG in the tissue section. This can lead to background staining. For the two-step ImmPRESS Excel Amplified Polymer Staining Kit, the species of the Amplifier Antibody (made in goat) should be considered in addition to the primary and tissue species.
The following options can minimize background staining in these instances:
Lung: aSMA (m), ImmPRESS-AP Anti-Mouse IgG, ImmPACT® Vector® Red AP Substrate. Counterstained with Hematoxylin (blue). Image kindly provided by the Centre for Inflammation and Tissue Repair, University College London.
Canine small intestine: Cytokeratin (MNF 116), ImmPRESS™ VR Anti-Mouse IgG and Vector® DAB Substrate (brown). Counterstained with hematoxylin (blue).
|Product||Peroxidase||Veterinary Reagents (VR)||Excel Amplified (Peroxidase)||Alkaline Phosphatase|
|ImmPRESS™ Anti-Rabbit IgG Kit (made in horse)||MP-7401||MP-6401||MP-7601||MP-5401|
|ImmPRESS™ Anti-Rabbit IgG Kit (made in goat)||MP-7451|
|ImmPRESS™ Anti-Mouse IgG Kit (made in horse)||MP-7402||MP-6402||MP-7602||MP-5402|
|ImmPRESS™ Anti-Mouse IgG Kit (made in goat)||MP-7452|
|ImmPRESS™ Anti-Mouse IgG, Rat Adsorbed, Kit (made in horse)||MP-7422|
|ImmPRESS™ Anti-Rat IgG Kit (made in horse)||MP-7404||MP-5404|
|ImmPRESS™ Anti-Rat IgG, Mouse Adsorbed, Kit (made in horse)||MP-7444||MP-5444|
|ImmPRESS™ Anti-Goat IgG Kit (made in horse)||MP-7405||MP-5405|
|ImmPRESS™ Universal Antibody Kit, Anti-Rabbit/Mouse Kit (made in horse)||MP-7500|