Vector Labs is closed to observe Independence Day (US Holiday) on Thursday, July 4th.
We will be back in the office on Friday. We will respond to emails upon our return. Have a wonderful day.

Immunohistochemistry Resource Guide

Not enough time read now ?

Ihc Guide Cover V Ihc Guide Cover V Vectorlabs Ihc guide cover v Vectorlabs

Table of Contents

Choosing an ImmPRESS® Polymer Kit

When choosing the optimal detection system for your application, consider not only the species of the primary antibody but also the species of the tissue under examination. If the species of the primary antibody and the species of the tissue are closely related (for example, rat and mouse), the secondary antibody may cross-react with endogenous IgG in the tissue section. This can lead to background staining. For the two-step ImmPRESS Excel Amplified Polymer Staining Kit, the species of the Amplifier Antibody (made in goat) should be considered in addition to the primary and tissue species.

The following options can minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (for example, ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the ImmPRESS M.O.M.® Kit for applications of mouse primary antibodies on mouse tissue.
  • Use ImmPRESS VR Polymer Detection Reagents, which eliminate off-target binding and thus increase signal specificity in these animal tissues: bovine, goat, sheep, swine, horse, cat, dog, rabbit, rat, mouse.
  • Since the Amplifier Antibody in both the ImmPRESS Excel Anti-Mouse IgG Kit and the ImmPRESS Excel Anti-Rabbit Kit is made in goat, these kits are not recommended for goat, bovine and sheep tissue – the ImmPRESS Excel Polymer Detection Reagent (an anti-goat IgG) may cross-react with endogenous immunoglobulins.
Lung Asma Lung Asma Vectorlabs Lung asma Vectorlabs
Lung: aSMA (m), ImmPRESS-AP Anti-Mouse IgG, ImmPACT® Vector® Red AP Substrate. Counterstained with Hematoxylin (blue). Image kindly provided by the Centre for Inflammation and Tissue Repair, University College London.
Canine Small Intestine Cytokeratin Canine Small Intestine Cytokeratin Vectorlabs Canine small intestine cytokeratin Vectorlabs
Canine small intestine: Cytokeratin (MNF 116), ImmPRESS VR Anti-Mouse IgG and Vector DAB Substrate (brown). Counterstained with hematoxylin (blue).
Product Peroxidase Veterinary Reagents (VR) Excel Amplified (Peroxidase) Alkaline Phosphatase
ImmPRESS® Anti-Rabbit IgG Kit (made in horse) MP-7401 MP-6401 MP-7601 MP-5401
ImmPRESS® Anti-Rabbit IgG Kit (made in goat) MP-7451      
ImmPRESS® Anti-Mouse IgG Kit (made in horse) MP-7402 MP-6402 MP-7602 MP-5402
ImmPRESS® Anti-Mouse IgG Kit (made in goat) MP-7452      
ImmPRESS® Anti-Mouse IgG, Rat Adsorbed, Kit (made in horse) MP-7422      
ImmPRESS® Anti-Rat IgG Kit (made in goat) MP-7404     MP-5404
ImmPRESS® Anti-Rat IgG, Mouse Adsorbed, Kit (made in goat) MP-7444     MP-5444
ImmPRESS® Anti-Goat IgG Kit (made in horse) MP-7405     MP-5405
ImmPRESS® Universal Antibody Kit, Anti-Rabbit/Mouse Kit (made in horse) MP-7500