Vector Laboratories will be closed on Monday, September 4, in observance of Labor Day. Any orders placed after 2pm PT on Friday, September 1, will be shipped on Tuesday, September 5. Thank you for your understanding.

Immunohistochemistry Resource Guide

Not enough time read now ?

Table of Contents

Enzyme Substrate Combinations

Recommended enzyme substrate combinations and the recommended order in which they should be used.

Key: Alkaline Phosphatase Peroxidase + Indicates good contrast – Indicates incompatiblity of substrates for various reasons
Breast Carcinoma: • Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® HRP substrate (red) • CD34 (m), VECTASTAIN ABC-AP Kit, Vector Blue AP Substrate (blue) • Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, Vector DAB HRP Substrate (brown).
Tonsil: • CD3 (m), ImmPRESS® Anti-Mouse IgG Reagent, Vector VIP HRP Substrate (purple) • Ki67 (m), ImmPRESS Anti-Mouse IgG Reagent, Vector SG HRP Substrate (blue/gray).
Prostate: • Cytokeratin 5 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue AP Substrate (blue) • CD34 (m), VECTASTAIN Universal ABC-AP Kit, Vector Red AP Substrate (red).

Multiple Labeling Examples

Colon Cancer: • Ki67 (rm), ImmPRESS-AP Anti-Rabbit IgG Reagent, Vector Blue AP Substrate (blue) • Cox2 (rm), ImmPRESS-AP Anti-Rabbit IgG Reagent, ImmPACT® Vector Red AP Substrate (red).
Colon: • Ki67 (rm), ImmPRESS Universal (Anti-Mouse/Anti-Rabbit IgG) HRP Reagent, Vector VIP (purple) • Cytokeratin (m), ImmPRESS Universal (Anti- Mouse/Anti-Rabbit IgG) HRP Reagent, Vector SG HRP Substrate (gray).
Breast Carcinoma: • Ki67 (rm), ImmPRESS Reagent (HRP; Universal), Vector DAB (brown) • CD34 (m), ImmPRESS Reagent (HRP; Universal), Vector VIP (purple).
Melanoma: • Cyclin A (m), ImmPRESS-AP Anti-Mouse IgG, ImmPACT Vector Red AP Substrate (magenta) • Melanoma Marker (m) ImmPRESS HRP Anti- Mouse IgG, Vector SG HRP Substrate (gray). Note contrast of double stain with the brown pigments in the tissue.
Tonsil: • CD3 (m), VECTASTAIN Elite ABC Kit (Universal), Vector DAB (brown) • CD20 (m), VECTASTAIN Elite ABC Kit (Universal), Vector SG (blue-gray) • Multi-Cytokeratin (m), VECTASTAIN Elite ABC Kit (Universal), Vector VIP (purple).
Breast Carcinoma: • Estrogen Receptor (rm), ImmPRESS Universal Reagent, Vector NovaRED HRP Substrate (red) • M2A Antigen (m), ImmPRESS Universal HRP Reagent, Vector DAB+Ni HRP Substrate (gray/black).
Colon: • M2A Antigen (m), ImmPRESS Universal (Anti-Mouse/Rabbit IgG) HRP Reagent, Vector NovaRED HRP Substrate (red) • CD34 (m), VECTASTAIN Universal ABC-AP kit, Vector Blue AP Substrate (blue).
Colon Carcinoma: • S100 (rp), VECTASTAIN Universal ABC-AP Kit, Vector Red AP Substrate (red) • Cytokeratin 8/18 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG HRP Substrate (blue/gray).