Immunohistochemistry Resource Guide

Table of Contents

Introduction

Immunohistochemistry Workflow

Immunohistochemistry Selection Guide

Pioneering in IHC Technology

Choosing a Detection System

Avidin-Biotin Complex (ABC)-Based Detection

VECTASTAIN® ABC Systems

VECTASTAIN® ABC Kits

Choosing a VECTASTAIN® ABC Kit

> Customizing your VECTASTAIN® ABC Kit

Polymer-Based Detection

ImmPRESS® One-Step Polymer Systems

> ImmPRESS® Two-Step Amplified Polymer Systems

> Choosing an ImmPRESS® Polymer Kit

> Multiple Antigen Labeling Simplified

Species on Species Detection

M.O.M.® (Mouse on Mouse) Immunodetection Kits

Choosing an Enzyme Substrate

Enzyme Substrates

> Enzyme Substrate Properties

Multiple Antigen Labeling

Enzyme Substrate Combinations

Counterstaining

> Counterstain/Substrate Compatibility Table

Blocking Background Signal

Secondary and Tertiary Detection Reagents

Mounting Media

Accessory Reagents

Enzyme Substrates

We offer researchers an array of both conventional and unique enzyme substrates that produce a broad range of colors. Our enzyme substrates require no dissolving of powders or tablets and are provided in convenient dropper bottles which are safe and easy to handle.

* Reaction product deposition not discreet and can be variable
** Longer incubation times increase sensitivity of the enzyme substrate

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