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Immunohistochemistry Resource Guide

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Table of Contents

Enzyme Substrate Properties

See also Enzyme Substrate Combinations for multiple antigen labeling and Counterstain/Substrate Compatibility.

Substrate Color Catalog Number Microscopy Mounting Contrast in Pigmented Tissue Multiple Labeling Heat Resistant*
Bright- field Darkfield Electron Fluore- scence Spectral Imaging
Peroxidase
Vector® DAB Brown SK-4100

 

Non-aqueous or Aqueous

 

Vector® DAB +Ni Gray-Black SK-4100

 

Non-aqueous

 

 

ImmPACT® DAB Brown SK-4105

 

Non-aqueous or Aqueous

 

ImmPACT® DAB EqV Brown SK-4103

 

Non-aqueous or Aqueous

 

Vector® VIP Purple SK-4600

 

Non-aqueous

 

ImmPACT® VIP Purple SK-4605

 

Non-aqueous

 

Vector® SG Blue-Gray SK-4700

 

Non-aqueous or Aqueous

 

ImmPACT® SG Blue-Gray SK-4705

 

Non-aqueous or Aqueous

 

Vector® NovaRED® Red SK-4800

 

Non-aqueous

 

ImmPACT® NovaRED® Red SK-4805

 

Non-aqueous

 

Vector® AEC Red SK-4200

 

 

 

Aqueous

 

ImmPACT® AEC Red SK-4205

 

 

 

Aqueous

 

ImmPACT® AMEC Red Red SK-4285

 

 

 

Aqueous

 

TMB Blue SK-4400

 

 

 

Non-aqueous

 

 

 

Alkaline Phosphatase
Vector® Red Magenta SK-5100

 

 

Non-aqueous or Aqueous

ImmPACT® Vector® Red

Magenta SK-5105

 

 

Non-aqueous or Aqueous

Vector® Blue Blue SK-5300

 

 

Non-aqueous or Aqueous

Vector® Black Brown-Black SK-5200

 

 

 

 

Non-aqueous

 

 

 

BCIP/NBT Indigo SK-5400

 

 

Non-aqueous or Aqueous

 

* Substrates that are designated “heat resistant” were developed on tissue then subjected to heat induced epitope retrival (HIER) using a pressure cooker technique (stained tissue was pressure cooked for 1 minute in Antigen Unmasking Solution, returned to room temperature, and rinsed in buffer). Resulting sensitivity after this treatment was found to be equivalent to non-HIER treated tissue.

Peroxidase
Substrates

Prostate Prostate Specific Antigen Brown Prostate Prostate Specific Antigen Brown Vectorlabs Prostate prostate specific antigen brown Vectorlabs
Prostate: Prostate Specific Antigen (m), ImmPRESS Reagent (HRP), ImmPACT DAB (brown).d), Hematoxylin QS (blue).
Tonsil Cytokeratin Ae Blue Gray Tonsil Cytokeratin Ae Blue Gray Vectorlabs Tonsil cytokeratin ae blue gray Vectorlabs
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP), ImmPACT SG (blue-gray).ylin QS (blue).
Tonsil Cd Purple Tonsil Cd Purple Vectorlabs Tonsil cd purple Vectorlabs
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP), ImmPACT NovaRED (red).EC (red), Hematoxylin QS (blue).
Tonsil Cytokeratin Ae Red Tonsil Cytokeratin Ae Red Vectorlabs Tonsil cytokeratin ae red Vectorlabs
Tonsil: CD20 (m), ImmPRESS Reagent (HRP), ImmPACT VIP (purple).
Tonsil Cytokeratin Ae Gray Black Tonsil Cytokeratin Ae Gray Black Vectorlabs Tonsil cytokeratin ae gray black Vectorlabs
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP), Vector DAB-Ni (gray-black).
.
Tumor Cytokeratin Blue Tumor Cytokeratin Blue Vectorlabs Tumor cytokeratin blue Vectorlabs
Tumor: Cytokeratin (s), VECTASTAIN® Elite® ABC Kit, TMB (blue).
Prostate Prostate Specific Antigen Red Prostate Prostate Specific Antigen Red Vectorlabs Prostate prostate specific antigen red Vectorlabs
Prostate: Prostate Specific Antigen (m), ImmPRESS Reagent (HRP), ImmPACT AEC (red).
.
Tonsil Lca Red Tonsil Lca Red Vectorlabs Tonsil lca red Vectorlabs
Tonsil: LCA (m), ImmPRESS Reagent (HRP), ImmPACT AMEC Red (red).

Alkaline Phosphatase Substrates

Tonsil Cytokeratin Ae Magenta Tonsil Cytokeratin Ae Magenta Vectorlabs Tonsil cytokeratin ae magenta Vectorlabs
Tonsil: Cytokeratin AE1/AE3 (m), Vector ImmPRESS- AP Reagent, ImmPACT Vector Red (magenta).
.
Tonsil Cytokeratin Ae Blue Tonsil Cytokeratin Ae Blue Vectorlabs Tonsil cytokeratin ae blue Vectorlabs
Tonsil: Cytokeratin AE1/AE3 (m), ImmPRESS-AP Reagent, Vector Blue (blue).
Colon Carcinoma Pan Cytokeratin Brown Black Colon Carcinoma Pan Cytokeratin Brown Black Vectorlabs Colon carcinoma pan cytokeratin brown black Vectorlabs
Colon Carcinoma: Pan-Cytokeratin (m), VECTASTAIN ABC-AP Kit, Vector Black (brown-black).
.
Prostate Prostate Specific Antigen Indigo Prostate Prostate Specific Antigen Indigo Vectorlabs Prostate prostate specific antigen indigo Vectorlabs
Prostate: Prostate Specific Antigen (m), VECTASTAIN ABC-AP Kit, BCIP/NBT (indigo).