Immunohistochemistry Resource Guide

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Table of Contents

Accessory Reagents

VECTABOND® Reagent Tissue Section Adhesive

VECTABOND Reagent chemically modifies the surface of glass to form a highly adherent charged surface. This charge significantly increases the adherence of both frozen and paraffin-embedded tissue sections and cell preparations to glass microscope slides and coverslips. Tissue sections will remain attached even when subjected to the most extreme conditions, such as high-temperature antigen retrieval and in situ hybridization. VECTABOND Reagent treated slides can be stored indefinitely.

ImmEdge® Hydrophobic Barrier Pen

The ImmEdge Pen is a hydrophobic barrier (PAP) pen for immunohistochemistry and in situ hybridization. It provides a water-repellent barrier that keeps IHC reagents localized on tissue specimens and prevents mixing of IHC reagents when multiple sections are mounted on the same slide.

  • Heat-stable
  • Insoluble in alcohol and acetone
  • Stable for use with buffers with and without detergent (Tween 20, Triton X-100, etc.)
  • Completely removed by all commonly used xylene and xylene-substitute clearing agents
  • Contains no ozone-depleting solvents
  • Compatible with both enzyme- and fluorescence-based detection systems

ImmPrint™ Histology Pen

The ImmPrint Histology Pen is a permanent marking pen designed for writing on glass microscope slides, tissue cassettes, and most hard surfaces. Unlike other pens commonly used for histology, the ImmPrint Pen has a smooth writing tip that resists drying out.

  • High-density, fast-drying, black ink
  • Resistant to most organic solvents encountered in histological applications


Control Antibodies

These antibodies are IgG preparations for use as controls for primary antibodies made in rabbit, mouse, rat, or goat. Each has been purified from pooled serum of healthy adult animals and contain a spectrum of the IgG subclasses. When applied appropriately, these controls will help determine whether the primary antibody staining signal is specific for the antigen or whether staining is the result of non-specific adsorption of primary antibody to tissue sites.

Antigen Unmasking Solutions

Our Antigen Unmasking Solutions are highly effective at revealing antigens in formalin-fixed, paraffin-embedded tissue sections when used in combination with a high temperature treatment procedure. We offer two formulations of Antigen Unmasking Solution: Citrate-based solution (pH 6.0) and Tris-based solution (pH 9.0), each supplied as 100X concentrated stocks.

Product Catalog Number
VECTABOND® Reagent (Tissue Section Adhesive) SP-1800-7
ImmEdge® Hydrophobic Barrier Pen H-4000
ImmPrint™ Histology Pen H-6100
Control Antibodies
Rabbit IgG I-1000-5
Mouse IgG I-2000-1
Rat IgG I-4000-1
Goat IgG I-5000-5
Antigen Unmasking Solutions
Citrate-based (100X) (pH 6.0) H-3300-250
Tris-based (100X) (pH 9.0) H-3301-250
Breast Carcinoma: Without (left panel) and with (right panel) Citrate-based Antigen Unmasking Solution, Estrogen receptor (m), ImmPRESS® Anti-Rabbit IgG Kit, DAB (brown) substrate. Hematoxylin QS (blue) counterstain.
Lymph Node: Without (left panel) and with (right panel) TRIS-based Antigen Unmasking Solution, Cyclin D1 (rm), ImmPRESS Anti-Rabbit IgG Kit, DAB (brown) substrate. Hematoxylin QS (blue) counterstain.

Contact Details

US Office

USA Headquarters:

Vector Laboratories, Inc.

6737 Mowry Ave
Newark, CA 94560
United States

Tel: +1 (650) 697-3600

Ordering and Technical Service (USA):+1 (800) 227-6666



International Inquiries: Please contact your local distributor