Immunohistochemistry Resource Guide

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Mounting Media

VectaMount® Permanent Mounting Medium is an optically clear formula for permanently preserving histochemical stains or precipitable enzyme substrates in tissue sections or cell preparations.

  • Non-aqueous
  • Toluene- and xylene-free (low toxicity)
  • Resin-based media
  • Odorless
  • Viscosity formulated for easy application and uniform spreading
  • Compatible with most horseradish peroxidase and alkaline phosphatase substrates
  • Dries clear with an ideal refractive index suitable for high-resolution oil-immersion microscopy

VectaMount AQ Aqueous Mounting Medium

VectaMount AQ Aqueous Mounting Medium preserves the color and clarity of enzyme substrates whose reaction products are soluble in alcohol or other organic solvents. Stained and mounted sections can be stored in a slide box at room temperature for at least two years without fading.

  • Hard-setting
  • Simple to use, requires no mixing
Product Catalog Number
VectaMount® Permanent Mounting Medium H-5000-60
VectaMount® AQ Aqueous Mounting Medium H-5501-60

Mounting Media / Substrate Compatibility

Substrate VectaMount® Permanent
Mounting Medium
VectaMount® AQ Aqueous
Mounting Medium
HRP
Vector® DAB

Vector® DAB-Ni

 
ImmPACT® DAB

ImmPACT® DAB EqV

Vector® VIP

 
ImmPACT® VIP

 
Vector® NovaRED®

 
ImmPACT® NovaRED®

 
Vector® SG

ImmPACT® SG

Vector® AEC  

ImmPACT® AEC  

ImmPACT® AMEC Red  

Vector® TMB

 
AP
Vector® Red

ImmPACT® Vector® Red

Vector® Blue

Vector® Black

 
Vector® BCIP/NBT

Melanoma: S100 (rp), VECTASTAIN® Elite® ABC Kit, Vector VIP (purple), Vector Methyl Green counterstain (green). Note color contrast with brown pigments in tissue.

Colon: • CD3 (rm), ImmPRESS® Reagent (HRP) Anti-Rabbit IgG, ImmPACT SG (blue-gray) • Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP) Anti-Mouse IgG, ImmPACT AMEC Red (red).

Cytospin of EBV+ cell line: Epstein-Barr virus nuclear antigen 1 (EBNA-1; rat), ImmPRESS Reagent (HRP) Anti-Rat IgG, ImmPACT NovaRED (red). (Image courtesy of Dr. GM Reynolds, Centre for Liver Research, University of Birmingham, U.K.)

Tumor: • CD34 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue AP Substrate (blue) • Cytokeratin 8/18 (m), VECTASTAIN Universal ABC-AP Kit, Vector Red AP Substrate (red).

Breast carcinoma: • CD31 (m), ImmPRESS Anti-Mouse IgG HRP Reagent, ImmPACT DAB EqV HRP Substrate (brown) • MRC1 (r), ImmPRESS- AP Anti- Rabbit IgG Reagent, ImmPACT Vector Red AP Substrate (magenta). Image courtesy of Richard Allen, part of the Academic Unit of Inflammation and Tumour Targeting headed by Professor Claire Lewis.

Melanoma: Vimentin (rm), ImmPRESS-AP Anti-Rabbit IgG Reagent, Vector Blue AP Substrate (blue). Note color contrast with brown pigments in tissue.