Immunohistochemistry Resource Guide

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Mounting Media

VectaMount™ Permanent Mounting Medium VectaMount™ Mounting Medium is an optically clear formula for permanently preserving histochemical stains or precipitable enzyme substrates in tissue sections or cell preparations.

  • Non-aqueous
  • Toluene- and xylene-free (low toxicity)
  • Resin-based media
  • Odorless
  • Viscosity formulated for easy application and uniform spreading
  • Compatible with most horseradish peroxidase and alkaline phosphatase substrates
  • Dries clear with an ideal refractive index suitable for high-resolution oil-immersion microscopy

VectaMount™ AQ Aqueous Mounting Medium

VectaMount™ AQ Aqueous Mounting Medium preserves the color and clarity of enzyme substrates whose reaction products are soluble in alcohol or other organic solvents. Stained and mounted sections can be stored in a slide box at room temperature for at least two years without fading.

  • Hard-setting
  • Simple to use, requires no mixing
ProductCatalog Number
VectaMount™ Permanent Mounting Medium H-5000
VectaMount™ AQ Aqueous Mounting Medium H-5501

Mounting Media / Substrate Compatibility

SubstrateVectaMount™ Permanent
Mounting Medium
VectaMount™ AQ Aqueous
Mounting Medium
HRP
Vector® DAB
Vector® DAB-Ni
ImmPACT™ DAB
ImmPACT™ DAB EqV
Vector® VIP
ImmPACT™ VIP
Vector® NovaRED™
ImmPACT™ NovaRED™
Vector® SG
ImmPACT™ SG
Vector® AEC
ImmPACT™ AEC
ImmPACT™ AMEC Red
Vector® TMB
AP
Vector® Red
ImmPACT™ Vector® Red
Vector® Blue
Vector® Black
Vector® BCIP/NBT

Melanoma: S100 (rp), VECTASTAIN® Elite® ABC Kit, Vector® VIP (purple), Vector® Methyl Green counterstain (green). Note color contrast with brown pigments in tissue.

Colon: • CD3 (rm), ImmPRESS™ Reagent (HRP) Anti-Rabbit IgG, ImmPACT™ SG (blue-gray) • Cytokeratin AE1/AE3 (m), ImmPRESS™ Reagent (HRP) Anti-Mouse IgG, ImmPACT™ AMEC Red (red).

Cytospin of EBV+ cell line: Epstein-Barr virus nuclear antigen 1 (EBNA-1; rat), ImmPRESS™ Reagent (HRP) Anti-Rat IgG, ImmPACT™ NovaRED™ (red). (Image courtesy of Dr. GM Reynolds, Centre for Liver Research, University of Birmingham, U.K.)

Tumor: • CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector® Blue AP Substrate (blue) • Cytokeratin 8/18 (m), VECTASTAIN® Universal ABC-AP Kit, Vector® Red AP Substrate (red).

Breast carcinoma: • CD31 (m), ImmPRESS™ Anti-Mouse IgG HRP Reagent, ImmPACT™ DAB EqV HRP Substrate (brown) • MRC1 (r), ImmPRESS™- AP Anti- Rabbit IgG Reagent, ImmPACT™ Vector® Red AP Substrate (magenta). Image courtesy of Richard Allen, part of the Academic Unit of Inflammation and Tumour Targeting headed by Professor Claire Lewis.

Melanoma: Vimentin (rm), ImmPRESS™-AP Anti-Rabbit IgG Reagent, Vector® Blue AP Substrate (blue). Note color contrast with brown pigments in tissue.

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