Immunohistochemistry Resource Guide

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Secondary and Tertiary Detection Reagents

Our secondary antibodies are prepared by hyper-immunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low-affinity antibodies. Cross-reactivities that can interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality-control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis, solidphase binding assays and IHC tissue staining. These unconjugated antibodies are used to generate our enzyme conjugated and biotinylated secondary antibodies.

Biotinylated and Unconjugated Secondary Antibodies

Our high-affinity, purified, biotinylated and unconjugated secondary antibodies are manufactured under controlled conditions to retain maximum specificity and affinity. Our secondary antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blotting.

Secondary Antibodies Biotinylated Unconjugated
Host Species (Concentrate) Host Species (R.T.U.)† Host Species (Concentrate)
Goat Rabbit Horse Goat Horse Goat Rabbit Horse
Anti-Cat IgG (H+L) BA-9000-1.5              
Anti-Chicken IgG (H+L) BA-9010-1.5              
Anti-Goat IgG (H+L)   BA-5000-1.5 BA-9500-1.5   BP-9500-50   AI-5000-1.5  
Anti-Guinea Pig IgG (H+L) BA-7000-1.5              
Anti-Hamster IgG (H+L) BA-9100-1.5         AI-9100-1.5    
Anti-Horse IgG (H+L) BA-8000-1.5              
Anti-Mouse IgG (H+L) BA-9200-1.5   BA-2000-1.5 BP-9200-1.5 BP-2000-50 AI-9200-1.5   AI-2000-1.5
Anti-Mouse IgG (H+L), rat adsorbed     BA-2001-.5          
Anti-Mouse IgM (H+L), Mu chain specific BA-2020-.5              
Anti-Rabbit IgG (H+L) BA-1000-1.5   BA-1100-1.5 BP-9100-50 BP-1100-50 AI-1000-1.5    
Anti-Rat IgG (H+L) BA-9400-1.5 BA-4000-1.5   BP-9400-50     AI-4000-1.5  
Anti-Rat IgG (H+L), mouse adsorbed BA-9401-.5 BA-4001-.5         AI-4001-.5  
Anti-Sheep IgG (H+L)   BA-6000-1.5            
Universal Anti-Mouse/Rabbit IgG (H+L)     BA-1400-2.1   BP-1400-50      
Universal Pan-Specific Anti-Mouse/Rabbit/Goat IgG (H+L)     BA-1300-2.2          

† Ready-to-use, prediluted stabilized solutions

Anti-Human
Secondary Antibodies
Biotinylated Unconjugated
Host Species (Concentrate) Host Species (Concentrate)
Goat Goat
Anti-Human IgG (H+L) BA-3000-1.5 AI-3000-1.5
Anti-Human IgE, ε (Epsilon) chain specific BA-3040-.5  
Anti-Human IgG, γ (Gamma) chain specific BA-3080-.5 AI-3080-.5
Anti-Human IgM, μ (Mu) chain specific BA-3020-.5 AI-3020-.5
Anti-Human κ (Kappa) Chain, kappa chain specific BA-3060-.5 AI-3060-.5

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Enzyme Conjugated Secondary Antibodies

Our high-affinity, purified antibodies are cross-linked with alkaline phosphatase (AP) or horseradish peroxidase (HRP) of the highest specificity. Our conjugation method ensures the maximum preservation of enzyme activity and antibody specificity. Recommended applications include tissue staining, ELISAs, and blotting.

Product Catalog Number
Alkaline Phosphatase
Anti-Mouse IgG (H+L) made in horse Alkaline Phosphatase labeled AP-2000-1
Anti-Rabbit IgG (H+L) made in goat Alkaline Phosphatase labeled AP-1000-1
Peroxidase
Anti-Mouse IgG (H+L) made in horse Peroxidase labeled PI-2000-1
Anti-Rabbit IgG (H+L) made in goat Peroxidase labeled PI-1000-1
Anti-Human IgG (H+L) made in goat Peroxidase labeled PI-3000-1
Anti-Goat IgG (H+L) made in horse Peroxidase labeled PI-9500-1

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Avidin and Streptavidin Enzyme Conjugates

Our enzyme-conjugated avidin and streptavidin are suitable for use in solid-phase assays, tissue/cell staining systems, and blotting. The conjugates are produced in optimized ratios with enzymes of the highest specific activity. Covalent linkages are specifically chosen to provide stable, highly active conjugates.

Product Catalog Number
Alkaline Phosphatase
Alkaline Phosphatase Streptavidin SA-5100-1
Alkaline Phosphatase Avidin D A-2100-100
Peroxidase
Horseradish Peroxidase Streptavidin, concentrate SA-5004-1
Horseradish Peroxidase Streptavidin, R.T.U. SA-5704-100
Horseradish Peroxidase Avidin D, concentrate A-2004-5