Immunohistochemistry Resource Guide

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Secondary and Tertiary Detection Reagents

Our secondary antibodies are prepared by hyper-immunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low-affinity antibodies. Cross-reactivities that can interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality-control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis, solidphase binding assays and IHC tissue staining. These unconjugated antibodies are used to generate our enzyme conjugated and biotinylated secondary antibodies.

Biotinylated and Unconjugated Secondary Antibodies

Our high-affinity, purified, biotinylated and unconjugated secondary antibodies are manufactured under controlled conditions to retain maximum specificity and affinity. Our secondary antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blotting.

Secondary AntibodiesBiotinylatedUnconjugated
Host Species (Concentrate)Host Species (R.T.U.)†Host Species (Concentrate)
Anti-Cat IgG (H+L) BA-9000
Anti-Chicken IgG (H+L) BA-9010
Anti-Goat IgG (H+L) BA-5000 BA-9500 BP-9500 AI-5000
Anti-Guinea Pig IgG (H+L) BA-7000
Anti-Hamster IgG (H+L) BA-9100 AI-9100
Anti-Horse IgG (H+L) BA-8000
Anti-Mouse IgG (H+L) BA-9200 BA-2000 BP-9200 BP-2000 AI-9200 AI-2000
Anti-Mouse IgG (H+L), rat adsorbed BA-2001
Anti-Mouse IgM (H+L), Mu chain specific BA-2020
Anti-Rabbit IgG (H+L) BA-1000 BA-1100 BP-9100 BP-1100 AI-1000
Anti-Rat IgG (H+L) BA-9400 BA-4000 BP-9400 AI-4000
Anti-Rat IgG (H+L), mouse adsorbed BA-9401 BA-4001 AI-4001
Anti-Sheep IgG (H+L) BA-6000
Anti-Swine IgG (H+L) BA-9020
Universal Anti-Mouse/Rabbit IgG (H+L) BA-1400 BP-1400
Universal Pan-Specific Anti-Mouse/Rabbit/Goat IgG (H+L) BA-1300

† Ready-to-use, prediluted stabilized solutions

Secondary Antibodies
Host Species (Concentrate)Host Species (Concentrate)
Anti-Human IgG (H+L) BA-3000 AI-3000
Anti-Human IgE, ε (Epsilon) chain specific BA-3040 AI-3040
Anti-Human IgG, γ (Gamma) chain specific BA-3080 AI-3080
Anti-Human IgM, μ (Mu) chain specific BA-3020 AI-3020
Anti-Human κ (Kappa) Chain, kappa chain specific BA-3060 AI-3060

Enzyme Conjugated Secondary Antibodies

Our high-affinity, purified antibodies are cross-linked with alkaline phosphatase (AP) or horseradish peroxidase (HRP) of the highest specificity. Our conjugation method ensures the maximum preservation of enzyme activity and antibody specificity. Recommended applications include tissue staining, ELISAs, and blotting.

ProductCatalog Number
Alkaline Phosphatase
Anti-Mouse IgG (H+L) made in horse Alkaline Phosphatase labeled AP-2000
Anti-Rabbit IgG (H+L) made in goat Alkaline Phosphatase labeled AP-1000
Anti-Mouse IgG (H+L) made in horse Peroxidase labeled PI-2000
Anti-Rabbit IgG (H+L) made in goat Peroxidase labeled PI-1000
Anti-Human IgG (H+L) made in goat Peroxidase labeled PI-3000
Anti-Goat IgG (H+L) made in horse Peroxidase labeled PI-9500

Avidin and Streptavidin Enzyme Conjugates

Our enzyme-conjugated avidin and streptavidin are suitable for use in solid-phase assays, tissue/cell staining systems, and blotting. The conjugates are produced in optimized ratios with enzymes of the highest specific activity. Covalent linkages are specifically chosen to provide stable, highly active conjugates.

ProductCatalog Number
Alkaline Phosphatase
Alkaline Phosphatase Streptavidin SA-5100
Alkaline Phosphatase Avidin D A-2100
Horseradish Peroxidase Streptavidin, concentrate SA-5004
Horseradish Peroxidase Streptavidin, R.T.U. SA-5704
Horseradish Peroxidase Avidin D, concentrate A-2004
Horseradish Peroxidase Avidin D, R.T.U. A-2704