VectaMount® Mounting Media for IHC

Mounting media optimized for immunohistochemistry and immunocytochemistry

Using the correct mounting medium preserves your histochemical stains or precipitable enzyme substrates in tissue sections or cell preparations.

VectaMount® Express Mounting Medium

  • Non-aqueous clearing and mounting medium for IHC-stained slides
  • Eliminates the need for extensive ethanol washes and solvent-based clearing agents (e.g., xylene) 
  • Rapid drying formula for fast visualization of stained samples
  • Preserves staining for at least 18 months at room temperature
  • Compatible with horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme substrates, as well as commonly used counterstains
  • Optically clear formula with a refractive index of 1.49, resulting in high-quality imaging free of distortion or artifact

VectaMount® Permanent Mounting Medium

  • Non-aqueous mounting medium
  • Odorless and toluene- and xylene-free (low toxicity)
  • Resin-based
  • Viscosity formulated for easy application and uniform spreading
  • Compatible with most horseradish peroxidase (HRP) and alkaline phosphatase (AP) substrates
  • Dries clear with a refractive index ideal for high-resolution oil-immersion microscopy

VectaMount AQ Aqueous Mounting Medium

  • Hardsetting, aqueous mounting medium
  • Preserves the color and clarity of enzyme substrates whose reaction products are soluble in alcohol or other organic solvents
  • Simple to use, requires no mixing
  • Stained sections mounted with VectaMount AQ can be stored in a slide box at room temperature for at least two years without fading

Substrate / VectaMount Compatibility Table

This table is designed as a reference to help you determine the optimal mounting medium for your application.

Substrate Catalog
Number
VectaMount
Express
VectaMount
Permanent
VectaMount
AQ Aqueous

Horseradish Peroxidase (HRP)

ImmPACT DAB (brown)

SK-4105

ImmPACT DAB EqV (brown)

SK-4103

DAB (brown)

SK-4100

DAB-Ni (gray-black)

SK-4100

 

ImmPACT AEC (red)

SK-4205  

 

ImmPACT AMEC Red (red)

SK-4285  

 

AEC (red)

SK-4200  

 

TMB (blue)

SK-4400  

 

ImmPACT VIP (purple)

SK-4605

 

Vector VIP (purple)

SK-4600

 

ImmPACT SG (blue-gray)

SK-4705

Vector SG (blue-gray)

SK-4700

ImmPACT NovaRED (red)

SK-4805

 

Vector NovaRED (red)

SK-4800

 

Alkaline Phosphatase (AP)

ImmPACT Vector Red (magenta)

SK-5105

Vector Red (magenta)

SK-5100

Vector Black (black)

SK-5200

 

 

Vector Blue (blue)

SK-5300

BCIP / NBT (indigo)

SK-5400

 

       

Resources

Immunohistochemistry Resource Guide

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
Download

IHC Troubleshooting Guide for ABC Systems

A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol using ABC systems.  Also troubleshoot issues of weak or no staining. Laminated.

Download

IHC Troubleshooting Guide for Polymer Kits

A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol polymer detection systems.  Also troubleshoot issues of weak or no staining. Laminated.

Download