Mounting Media for IHC

Mounting media optimized for immunohistochemistry and immunocytochemistry

Using the correct mounting medium preserves your histochemical stains or precipitable enzyme substrates in tissue sections or cell preparations.

VectaMount® Permanent Mounting Medium

  • Non-aqueous mounting medium
  • Odorless and toluene- and xylene-free (low toxicity)
  • Resin-based
  • Viscosity formulated for easy application and uniform spreading
  • Compatible with most horseradish peroxidase (HRP) and alkaline phosphatase (AP) substrates
  • Dries clear with a refractive index ideal for high-resolution oil-immersion microscopy

VectaMount AQ Aqueous Mounting Medium

  • Hardsetting, aqueous mounting medium
  • Preserves the color and clarity of enzyme substrates whose reaction products are soluble in alcohol or other organic solvents
  • Simple to use, requires no mixing
  • Stained sections mounted with VectaMount AQ can be stored in a slide box at room temperature for at least two years without fading

Substrate / VectaMount Compatibility Table

This table is designed as a reference to help you determine the optimal mounting medium for your application.

Substrate Catalog Number VectaMount® Permanent
Mounting Medium
VectaMount® AQ Aqueous
Mounting Medium

Horseradish Peroxidase (HRP)

ImmPACT DAB (brown)

SK-4105

ImmPACT DAB EqV (brown)

SK-4103

DAB (brown)

SK-4100

DAB-Ni (gray-black)

SK-4100

 

ImmPACT AEC (red)

SK-4205

 

ImmPACT AMEC Red (red)

SK-4285

 

AEC (red)

SK-4200

 

TMB (blue)

SK-4400

 

ImmPACT VIP (purple)

SK-4605

 

Vector VIP (purple)

SK-4600

 

ImmPACT SG (blue-gray)

SK-4705

SG (blue-gray)

SK-4700

ImmPACT NovaRED (red)

SK-4805

 

Vector NovaRED (red)

SK-4800

 

Alkaline Phosphatase (AP)

ImmPACT Vector Red (magenta)

SK-5105

Vector Red (magenta)

SK-5100

Vector Black (black)

SK-5200

 

Vector Blue (blue)

SK-5300

BCIP / NBT (indigo)

SK-5400


Resources

Immunohistochemistry Resource Guide

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
Learn more about this guide >

IHC Troubleshooting Guide for ABC Systems

A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol using ABC systems.  Also troubleshoot issues of weak or no staining. Laminated.

Download

IHC Troubleshooting Guide for Polymer Kits

A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol polymer detection systems.  Also troubleshoot issues of weak or no staining. Laminated.

Download