VECTOR VIP Peroxidase (HRP) Substrate Kit

Cat. No: SK-4600
Price: $144.00
Unit Size: 1 kit

Overview

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Permanent mounting
  • Ideal for single and multiple labeling
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • One year expiry date
  • Sufficient reagents to produce 300 ml of working solution

Product review on Biocompare.com.

1.  Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122

Cat. No. SK-4600
Unit Size 1 kit
Country of Manufacture United States
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Purple
Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate Properties Contrast with Tissue Pigments, Multiple Labeling
Mounting Non-Aqueous (Permanent)

More Information

Vector® VIP and ImmPACT™ VIP HRP substrates both produce intense, violet (purple) reaction products, and can be used as alternatives to DAB or as a second color for multiple antigen labeling. Vector® VIP and ImmPACT™ VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector® VIP and ImmPACT™ VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.

Vector® VIP Substrate Kit contains stock solutions in convenient dropper bottles. The sensitivity of Vector® VIP substrate is equivalent to Vector® DAB Substrate. This product can also be used on blots. The kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4° C, this kit is stable for one year.

Product review on Biocompare.com.

1.  Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122

Relative Sensitivity of Substrates

Substrate Combinations

 

Counterstain Compatibility