TMB Substrate Kit, Peroxidase (HRP), (3,3', 5,5'-tetramethylbenzidine)

SKU Unit Size Price Qty
SK-4400 1 kit

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TMB HRP Substrate Kit (SK-4400) contains reagents in convenient dropper bottles necessary to produce either a precipitating blue reaction product or a soluble blue reaction product. This kit contains a stabilizing solution which, when added to the working solution, allows the development of a blue precipitate.


  • Greater sensitivity than conventional substrates
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 300 ml of working solution


More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Blue
Microscopy Brightfield, Spectral Imaging
Mounting Non-Aqueous (Permanent)
Safety Title WARNING: Cancer -

Kit Components

Kit Contents:

  • 6 ml TMB Reagent 1
  • 6 ml TMB Reagent 2
  • 6 ml TMB Reagent 3
  • 6 ml TMB Reagent 4


Technical Information

This sensitive chromogen can be used for blotting, immunohistochemical, and in situ hybridization applications. TMB stained slides can be dehydrated and permanently mounted. The soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays.


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